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...infected with the lentivirus HIV-1. Today, HIV-1 can be controlled with antiviral therapies such that ...from infected cells. Read on to learn how CRISPR can take on HIV-1 in vitro, and what obstacles must be...healthy individuals. Antiretroviral therapy (ART) can virtually eliminate plasma HIV-1, improving life ...promoter-driven GFP expression. In cell lines that can produce HIV-1 toxic proteins, CRISPR/Cas9 SAM caused...our reach. Similar studies have shown that CRISPR can be used to combat other viral infections, notably...learned in pursuing one technological application can benefit many others and accelerate the pace of research...inflammation, and negative metabolic effects of ART. Why can’t the body mount an immune response to destroy these...

...Without Cre recombination, RFP is expressed. Cre can mediate one of two deletions to allow YFP or M-CFP...using 2 loxP variants (only identical loxP sites can mediate recombination.) The deletions are mutually...deletion and inversion. The Brainbow-2.1 construct can express one of four colors (n-GFP, RFP, YFP or M-... two color possibilities. The remaining cassette can invert as long as Cre remains expressed; once Cre...includes a mutant YFP that does not fluoresce, but can be detected via immunostaining. This feature facilitates...placed under different promoters, Brainbow and Flpbow can be used to label distinct cell populations. To decrease...three recombinations, and subsequent recombinations cannot occur. While the Brainbow-1 system employs Cre-...

... approaches everything; we asked ourselves, “How can we do this in a way that most helps scientists and...about virus production protocols and quality control can be found on Addgene’s Viral Production webpage). ...partnerships to expand the service and figure out what we can afford to do and when. Addgene’s Legal Team acquired...Requested Lentivirus and AAV of 2016 Learn How You Can Use the Viral Service in Your Research Using Virus...

... promoters, has limited the number of gRNAs that can be packaged into viral vectors for potential in vivo...the Gao Lab demonstrated that the tRNA-gRNA array can drive the expression of itself, without using any...gene editing with DAP array   Use DAP arrays can enable efficient multiplex gene editing, such as ...different tRNAs with optimized 5’ leader sequences can be used together in DAP array. To construct DAP array...their initial testing. It outperformed other tRNA candidates (either from human or plant sources) in both ...

...a variety of roles in synaptic communication and can trigger other forms of neuronal signaling and regulation... The yellow (v82) and lime green (v857) versions can be summarized quickly as: iGluSnFR3 v82: increased...being deposited at Addgene. Other plasmids and pAAVs can be ordered directly from Addgene. The Podgorski Lab...provided a brief breakdown of the promoter systems you can use to express the iGluSnFR3 variants: pRSET: Bacterial...

...space per user, but, if you need more space, you can sign up for a plan. Best of all, by not using paper...also a very helpful aspect to this app; the user can search by sequence, category of enzyme, or name to..., and structures into one convenient resource. I can safely say that I still have the single and triple...codon list for each amino acid, but, overall AMINO can be beneficial for new researchers or students beginning...your notebook. The files uploaded are saved and cannot be deleted, so there is no worry about misplacing...find an MSDS for a lab chemical of choice. Now, I can’t guarantee every reagent in your chemical closet ...quizzed randomly about a hazardous chemical and can’t find your lab’s MSDS book, this is a great resource...

...foods, and fibers. And because working with plants can be challenging, there are a lot of unexplored areas...not food, not medicine, and not mandatory – people can decide for themselves if they want this beautiful...body of knowledge. Organizations like Open Plant can then build on this research and effectively carry... pursuing scientific or business enterprises, he can be found climbing mountains or playing with his two...plant biology is a significant technical obstacle to plant synthetic biology. We cannot build what we do...now synonymous with GM to the point that people cannot imagine any use for GM technology other than to...sales. Flowers that change color throughout the day cannot be developed through conventional breeding; synthetic...

...manuscript needs to be revised before it can be published, this can delay publication and manuscripts may...that the manuscript requires revisions, the authors can publish a new version of the article, while the first...effort) and the scientific community, as the reports can be very informative as to how a paper is received...journals and their scientific communities aim to significantly improve the reproducibility and reliability ...

...suggestions. Can’t find a plasmid you’re looking for in the repository? Let us know! You can notify us to...plasmid and a publication where we can find the plasmid. While we cannot guarantee that everyone we reach...

...applications, and the quality of a standard curve can make or break an experiment. Here, we will provide... similar buffers if not, since buffer components can affect the final readout. When preparing a standard...user, equipment, assay incubation time, etc., which can vary even when the same protocol is being followed...sigmoidal. Once the trendline is calculated, you can extrapolate the concentration of the unknown sample...lowest and highest concentrations that the curve can accurately measure.  Dynamic range depends on a number...