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Addgene

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We narrowed to 892 results for: CAN

Showing: 601 - 610 of 892 results

  1. Great Results Start with Great Standard Curves

    Type
    Blog Post
    Published
    Aug. 15, 2024, 1:15 p.m.
    ...applications, and the quality of a standard curve can make or break an experiment. Here, we will provide... similar buffers if not, since buffer components can affect the final readout. When preparing a standard...user, equipment, assay incubation time, etc., which can vary even when the same protocol is being followed...sigmoidal. Once the trendline is calculated, you can extrapolate the concentration of the unknown sample...lowest and highest concentrations that the curve can accurately measure.  Dynamic range depends on a number...

  2. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    Published
    Oct. 23, 2018, 11:49 a.m.
    ...purified virus. This process can be completed in one long day, or the virus can be stored at 4 °C and buffer...separate into two phases. If this happens, the solution can be cooled and the two phases mixed together. Lastly...This approach gives me a bit of flexibility since I can store the cell lysates at -80 °C for up to 6 months...SYBR green technology or a TaqMan primer/probe set can be used and samples are quantified by comparing to...and Harshana de Silva Feelixge at Fred Hutchinson Cancer Center for teaching me about AAV production and...

  3. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    Published
    Jan. 23, 2020, 2:45 p.m.
    ...reusable software components that can be incorporated into software. You can think of these libraries as ingredients...educational, and professional backgrounds. Sometimes this can lead to wildly different ideas of how to approach... our software. What this means for us is that we can focus on the meat of the software and not waste time... places, we might be calling it an aliquot. This can lead to confusion as to what the code is supposed...development happening at Addgene under the hood. A significant portion of our development may not be visible...average of 40 minutes to 10 minutes. While users can’t tangibly see these improvements, you are benefited... been updated in several years) or they make significant changes that make it incompatible with our software...

  4. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...channels. Since a Nextflow process can utilize any scripting language that can be executed on Linux, we created...computer, so it safely operates in isolation, and can be easily shared with others without any complicated...process defines which command or script will run and can be thought of as a particular step in the overall...step in our pipeline together.  Since each process can use a unique container, we customized each container...

  5. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ...town, and other Cas proteins like SaCas9 and Cpf1 can circumvent the limitations associated with SpCas9...activation of the fluorophore. The fluorescent signal can be used as an indicator to determine whether the ...its structure and function? For starters, Cas13a can be used to bind and cleave target RNAs, although ...the exciting discovery that some Cas13a orthologs can function in mammalian and plant cells (Abudayyeh ...the protein in mammalian cells. LwaCas13a-msfGFP can mediate cleavage with spacers from 20-28 nucleotides...From this analysis, they identified 53 potential candidate genes that fell into 3 categories based on the...question: C2c1, C2c2 and C2c3 (short for Class 2, candidate 1, 2, or 3). C2c1 and C2c3 are related to Cpf1...

  6. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ... 2021) can help restrict expression to your cell type of interest, while miRNA target sites can be used...retrograde transport in neurons. (b) Genetic cargo can be customized to control transgene expression. Gene... cell type specificity. An on/off genetic switch can be used for expression control. (c) Viral delivery...which is one of a handful of natural serotypes that can cross the BBB. However, these natural serotypes (...Systemic capsids packaging Cre-dependent transgenes can also be paired with Cre-expressing transgenic mouse...spatiotemporal control of transgene expression or activity can be achieved using switches, including riboswitches...use two or more AAVs to transduce the same cell, can accommodate larger, modular genetic payloads. Since...

  7. Our Most Popular Blog Posts from 2019

    Type
    Blog Post
    Published
    Dec. 31, 2019, 2:26 p.m.
    ...fluorescent proteins, careers, and more. These resources can now be found in one place: the Educational Resources...Graphics by Beth Kenkel Find a round-up of tools you can use for making scientific graphics. PS: they’re free... transcriptase and a prime editing guide RNA, it can introduce insertions, deletions, and all possible...

  8. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ...of the virus. While this can be useful, if too many neurons are labeled, it can be difficult to accurately...deficient (i.e. they cannot easily spread between cells like natural RABV or HSV can). AAV does not have...inserted electrodes) may be applied, but these methods can have a negative impact on cellular health. Another...Virus (HSV) also migrates through neurons where it can replicate and by doing so spread across several synaptic...immunogenicity and cellular toxicity. AAV vectors can be equipped with strong promoters for high and neuron-specific...fluoresce in different colors. An individual neuron can then be traced throughout the brain based on its ...Oh et al., 2014). Images yielded from this study can be found on the Allen Mouse Brain Connectivity Atlas...

  9. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ...expresses the HIV gag, pol, rev, and tet genes. It can be used with 2nd or 3rd generation lentiviral vectors... proteins determine which types of cells a virus can infect. The VSV-G envelope protein enables this virus...BbsI restriction site, a pair of annealed oligos can be cloned to create a chimeric guide RNA. Find pX330...producing lentiviral and MuLV retroviral particles. It can be used with packaging plasmids such as pCMV-dR8.2...

  10. Hot Plasmids - February 2022

    Type
    Blog Post
    Published
    Feb. 22, 2022, 2:15 p.m.
    ... acids, HA is nine), multiple repeats of the tag can recruit multiple frankenbodies to amplify the signal...two guides require two arrays for Cas9, two guides can be streamlined into a single array for Cas12a. Image...histidine tags, are optimized for SDS-PAGE gels and can be detected on Western blots via engineered immunoglobulin...editor allows for base editing, while gene editing can be done via a nuclease-active CasMINI. This small...is highly specific in mammalian cells without significant off-target effects, yet small enough to be packaged...
Showing: 601 - 610 of 892 results