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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 790 results for: Cre

Showing: 611 - 620 of 790 results

  2. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Oct. 14, 2015, 2:30 p.m.
    ...PAM site, Cpf1 cleaves in a staggered fashion, creating a 5 nucleotide 5’ overhang 18-23 bases away from...mediated editing, as Kleinstiver et al. saw no decrease in Cpf1 activity with 4-6 base deletions at the...

  3. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    Published
    Sept. 3, 2015, 2:30 p.m.
    ...EVER confirmed this with me). While things were incredibly busy in those early years, the founders knew ...aspect of Addgene. As our size has drastically increased, it would have been a tight squeeze for all of...

  4. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ...the presence of your targeted nucleic acid. To increase the sensitivity of the assay, targeted DNA or ...non-specific (trans) ssDNA and harnessed this ability to create a DNA detection platform called DETECTR. How does...

  5. Lab to Office Culture Shock

    Type
    Blog Post
    Published
    Feb. 5, 2019, 1:04 p.m.
    ... you when you need it. The opportunity to be creative and think critically. One of the very first times...defined as sitting down at a computer and just creating, which for many scientists is the most enjoyable...

  6. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...gene disruption in Mycobacterium abscessus, an increasingly common human pathogen notorious for its high...adhesion to a PILATeS-functionalized coverslip creates mechanical tension, and the luminescence colocalizes...

  7. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    Published
    July 30, 2024, 1:15 p.m.
    ...released Viral Vectors 101 2nd edition — I was incredibly excited when we picked our viral vector service...GCaMP6. It could be a matter of the size of the incremental improvements between generations: jGCaMP8 allows...

  8. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    Published
    Nov. 10, 2016, 3:30 p.m.
    ...microbes would be affected (possibly killed), thus increasing the initial microbe’s chances of survival. This...antibiotic-free, farmed meats. We can focus on creating new antibiotic therapies, and investigate more...

  9. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    Published
    Oct. 20, 2016, 2:30 p.m.
    ...trolling: The art of deliberately, cleverly, and secretly pissing people off, usually via the internet, ...excellent. Finally, as I said in the AMA, it’s incredibly important to find good mentors and incentivize...

  10. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...line you will need to grow this initial stock and create a set of vials of ‘Master’ stock, which can be ...thawed, it is time to reorder this cell line to create new Master and Working stocks to work from. The...
Showing: 611 - 620 of 790 results