We narrowed to 803 results for: Cre

... you have either made a pipetting error or an incredible discovery. The range of RQ values used to call...to be amplified in the same reaction. Taqman’s increased sensitivity allows for a more confident determination...

...This amplicon can be used in molecular cloning to create a plasmid of interest, for detecting the presence...detected via a fluorescent signal. The signal increases proportionally to the amount of product produced...

...better suited for genome-wide CRISPRi library screening experiments where maintaining library diversity...of antibiotics are often essential genes which creates a challenge when studying them since their complete...

...the sequence inserted by HDR may inadvertently re-create a match for the active guide. With these constraints.... To use this tool, simply follow these steps: Create a new experiment in the program Design gRNAs by...

...of both while limiting disadvantages led to the creation of induced pluripotent stem cells or iPSCs. Induced...research. With all these benefits, iPSCs have increasingly become the ideal experimental system for research...

...a lot of attention on social media. I gained a concrete example to use to promote future attempts and ...learning from the careers of others were key to creating my career path. Luckily, in grad school much of...

...gRNA.  While this methodology has proven to be incredibly valuable to scientists, there are some potential...Harrington, Lucas B., et al. "A thermostable Cas9 with increased lifetime in human plasma." Nature Communications8.1...

... plates due to the fact that beta-lactamase is excreted. Satellite colonies are small colonies of bacteria...Antibiotic Resistance Plasmids 101: Blue-White Screening Choosing Your Perfect Plasmid Backbone  ...

...epitopes previously hidden within the protein. Created with BioRender.   Permeabilization What is ...better at permeabilizing organellar membranes. Created with BioRender.   How do I choose?! Ok, so ...

... itself in terms of what they do with their discretionary money. They also usually have a good event-planning...students, postdocs, and other working scientists, creating a presentation for 8th graders was a little intimidating...