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TypeBlog PostPublishedMarch 5, 2019, 1:08 p.m....Harbor Protocols 2011.1 (2011): pdb-prot5546. Richardson, Alexander, et al. "Visualizing the Fate of Transplanted...
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3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells
TypeBlog PostPublishedSept. 5, 2017, 1:58 p.m....0.5% correctly edited alleles, when screening reasonable amounts of single cell clones. Furthermore, CRISPR... -
Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?
TypeBlog PostPublishedMay 10, 2016, 2:30 p.m....we’ve previously seen in the CRISPR field, the lessons learned in pursuing one technological application... -
Thoughts on the Future of Research 2015 Boston Symposium
TypeBlog PostPublishedNov. 25, 2015, 4 p.m....Future of Research (FOR) Boston Symposium 2015. I personally participated as a member of the Career Paths ... -
Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility
TypeBlog PostPublishedMay 18, 2018, 7:32 p.m....designs and we need to be transparent about the reasons we alter these designs if we do so. The most extreme... -
How to Design Your gRNA for CRISPR Genome Editing
TypeBlog PostPublishedSept. 24, 2020, 1:15 p.m....function. The best tools are only as good as the person using them, and the proper use of CRISPR technology... -
CRISPR 101: Off-Target Effects
TypeBlog PostPublishedFeb. 8, 2024, 2:15 p.m....sequence/whatever your editing event outcome is at a reasonable number, the likelihood of an off-target confounding... -
Antibodies 101: Multiplex Immunofluorescence
TypeBlog PostPublishedMay 23, 2023, 1:15 p.m.... M. A., Hoeppner, L. H., Ilangovan, V., Lo, H., Olson, M., Mohamed, S. Y., Sarabipour, S., Varma, A., ... -
Plasmids 101: Repressible Promoters
TypeBlog PostPublishedDec. 20, 2022, 2:15 p.m.... by Mary Gearing in 2018, and updated by Hannah Dotson in Dec. 2022. References and Resources References... -
Recombinase-based State Machines Enable Order-dependent Logic in vivo
TypeBlog PostPublishedJuly 28, 2016, 2:30 p.m....GRSMs, we recommend using overlap extension PCR and Gibson assembly to piece together the registers. All parts...