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We narrowed to 892 results for: CAN

Showing: 621 - 630 of 892 results

  1. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ...single stranded DNA degradation. Cas3 DNA digestion can proceed up to multiple kilobases, but only in one...of the size) and does not require a tracrRNA (it can self-process pre-crRNA into crRNA). The size and ...likely due to the lack of collateral cleavage). You can read about Cas7-11 in our blog post on its development...are worth taking a look at. Choosing the right Cas can really put your experiments Nsync.   References ...enzymes outlined below as well. Cas12a also has significant therapeutic promise due to the fact that it is...target sequence Similar to Cas12, it is also significantly smaller in size than traditional Cas9. Another... not display collateral RNA cleavage and is significantly less toxic to mammalian cells than Cas13 (likely...

  2. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type
    Blog Post
    Published
    Nov. 3, 2020, 2:15 p.m.
    ...This light-induced excitation can take place in a tissue culture dish but can also happen in real-time inside...still require fiber-optic cannula implantation, but altering ChRgers so they can be activated with tissue-penetrating...ChRgers, however, have a higher conductance, so they can still depolarize neurons at lower MOIs. Figure...the VTA is located in the midbrain, fiber-optic cannulas were still stereotaxically implanted into the ...mice’s skull near the M2 and secure a fiber optic cannula to the skull, leaving the brain intact. When the...

  3. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    Published
    Sept. 8, 2020, 2 p.m.
    ...channels typically expressed on other cell types can compensate for or bypass the dysfunction of another...mammalian cells to identify any solute channels that can compensate for the defect caused by SLC6A8 deficiency...hypothesized that this is abnormally deregulated in TS and can lead to cardiac developmental defects. Eight genes...example, GPCRs and their downstream G protein partners can regulate ion channels in the brain.  “I have a long...genes from the X chromosome are expressed at significantly reduced levels in women with TS (Trolle et al...vectors from Addgene, the lab will express these candidate genes in TS iPSCs to identify whether any rescue... use CRISPR plasmids to generate deletions of candidate genes in euploid iPSCs. “The BeHEARD Addgene reagents...

  4. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

    Type
    Blog Post
    Published
    May 5, 2015, 3:54 p.m.
    ...it works for me. I can often remember which paper I need to open, but when I can’t it’s an easy search...scientific literature are Google Scholar and PubMed. You can search keywords, titles, authors, year, narrow by...(relevance, dates etc). The links for the titles can generally take you to the full text of the article... same conclusion. Personally, I like papers that can end with some sort of model (cartoons are appreciated...Set aside some time each day or week to at least scan the titles of what’s going on in your research area...as soon as they are available. Give them a quick scan for interesting key words and download what seems...

  5. Reflections and Looking Ahead

    Type
    Blog Post
    Published
    Feb. 25, 2025, 2:15 p.m.
    ... in-person, you helped us understand how Addgene can better serve the global scientific community. As ...Almost any AAV transfer plasmid in our repository can now be packaged on demand in five distinct serotypes... — ‌and reinventing our search experience so you can find exactly what you need, faster. We’re also building...tell only part of the story. This year, we made significant strides in making science more accessible and...

  6. Embracing Serendipity: A Crucial Element in the PhD Journey

    Type
    Blog Post
    Published
    April 25, 2024, 1:15 p.m.
    ...spending time outdoors in nature or visiting museums can be excellent methods to detach from the constant ...is not just a stroke of luck; it's a mindset that can lead to unexpected discoveries and innovative solutions...serendipity in your lab research and PhD studies can enrich the depth and creativity of your work, while...

  7. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    Published
    May 18, 2017, 2:30 p.m.
    ...to biosensor creation, and custom FRET biosensors can be created using the cpFRET kit created by Olivier...enzymatic activity or phosphorylation over time. You can also use biosensors to follow processes such as protein...targeting signal or a transmembrane domain, biosensors can be targeted to a particular cellular compartment ...

  8. The Blue Flame Award: Celebrating Addgene's Most Requested Depositors

    Type
    Blog Post
    Published
    Nov. 14, 2016, 3:30 p.m.
    ...deposited in Addgene!” As you can imagine, answering plasmid requests can be a time-consuming chore for...benefits of sharing plasmids with us. One way we can gauge if people are happy depositors is if they deposit...

  9. Scientific Sharing in the Time of COVID-19: Databases and Resources

    Type
    Blog Post
    Published
    May 12, 2020, 1:15 p.m.
    ...we believe that by coming together, we can create a significant impact on both clinical care and public...collaboration as the best way that the scientific community can make progress towards understanding this disease....resources nationally and internationally such that we can advance COVID-19 research to hopefully help guide... COVID-19 Resources Canada site. To date, the portal has recruited 3,218 Canadian volunteers, and features...nationally and internationally COVID-19 Resources Canada is a database that provides information about reagents...research into COVID-19. While this program is based in Canada, much of the information is applicable to researchers...

  10. What's New in CRISPR - March 2020

    Type
    Blog Post
    Published
    March 10, 2020, 1:15 p.m.
    ... the complex enters the nucleus where dCas9-Mxi1 can repress gene expression. In the dark, the NLS is ...expression of dCas9). The lab demonstrated the system can also be used to downregulate multiple genes at once...modifiers such as TET1 with inactive Cas enzymes can alter gene expression via acetylation or demethylation...
Showing: 621 - 630 of 892 results