...nature18308   Additional resources on Addgene.org Addgene’s General Use Antibodies Antibody guide Antibody...applications. You can find FLAG antibodies on Addgene’s catalog including a mouse monoclonal (M2) as well... acid peptide (EQKLISEEDL) derived from the protooncogene of the same name. The tag has a net negative...clone used today! Goat, chicken, mouse, or rabbit? Addgene has you covered with monoclonal antibodies to Myc...both cleave after the DVPD sequence. Check out Addgene’s monoclonal antibodies (mouse, rabbit, and chicken... antibodies or guidance on designing a GFP tag Addgene has you covered!   Potential tag issues Tags have...Antibody protocols Additional resources on the Addgene blog Antibodies 101: Epitope availability Plasmids...

...Resources on addgene.org  Addgene's Viral Vectors Guide Addgene's Chemogenetics Guide Addgene's Optogenetics... our collection, on the Addgene eBook webpage.  More resources on the Addgene blog CRISPR 101 eBook en...

...Blotting Resources on Addgene.org Find plasmids encoding recombinant antibodies at Addgene ...an IF experiment. What is immunofluorescence? Generally speaking, IF is an application in which antibodies...iBiology). IHC and ICC are essentially just more general terms that can also refer to assays using non-fluorescent... visualize their target. These antibodies are generated against a specific antigen and then conjugated... more common is that secondary antibodies are generally polyclonal, which means that multiple secondary...amplification. Another reason is cost effectiveness. Generating and validating primary antibodies is a time consuming...antibodies that exist and you can begin to see why generating fluorophore-conjugated versions for each primary...

...of sample preparation for high-throughput next generation (NGS) sequencing have prevented laboratories ...As part of our mission, we’ve been working with Addgene to develop and apply our plexWell™ Library Preparation... for NGS-based sequencing and confirmation of Addgene’s large and growing collection of curated plasmids...are using this technique in our partnership with Addgene to sequence large numbers of plasmids. plexWell...does not vary by DNA input amount. plexWell™ + Addgene = high quality NGS-assembled plasmids Because of...information about the presence of non-plasmid DNA or heterogeneous and/or mixed clones present in the original ...original sample. At seqWell, we have worked with Addgene to develop a highly optimized bioinformatics pipeline...

...zebrafish, and mice. Second Generation iGluSnFR: SF-iGluSnFR In the second generation of iGluSnFR, the Looger...being deposited at Addgene. Other plasmids and pAAVs can be ordered directly from Addgene. The Podgorski ... for Synaptic Imaging However, the first two generations of iGluSnFR had limitations: they were not optimal...extrasynaptic glutamate release. Building on the previous generation of the SF-Venus-iGluSnFR (itself an improvement...IPTG induction to express soluble protein CAG: Generic strong promoter. Formulated as AAV-DJ for neuronal...References and Resources Additional Resources on the Addgene Blog Learn about other Fluorescent Biosensors ...Fluorescent Protein Featured Topic Page Resources at Addgene.org Find Fluorescent Protein Biosensors Browse All...

...Before I started writing for the Addgene blog, sharing Chemistry Cat memes was how I used social media...social media as a scientist. Now I write for the @Addgene blog & I'm my lab's resident #scicomm expert. What...using social media as a scientist started with the Addgene blog. I wrote my first blog post out of boredom...person in the lab. When I saw a request for an Addgene guest blogger post about colony PCR, I signed up...and accepted a position to work remotely as an Addgene Science Communication Intern. Developing science... using Twitter, I found this paper. I wrote an Addgene blog post about it and shared the paper with my... this panel, but my consistent writing for the Addgene blog (~1-2 blog posts per month) and use of Twitter...

...Additional Resources on the Addgene Blog Read Additional Cancer Blog Posts Addgene's Cancer Collection Pages...this multi-pronged attack. Inflammation and tumorigenesis The conference opened with a very interesting... between tumor and immune cells in real time. Addgene depositor Professor Max Krummel from UCSF showed...engineering (Ref 5)  as described by Blue Flame Addgene depositor Professor Michel Sadelain from the Memorial...poster is pTomo-Ras which has been deposited at Addgene was used to produce the glioblastoma model for ...addition, it was exciting to see that the use of optogenetic tools is expanding to immunological research....with the scientific community, contact deposit@addgene.org to find out more about our free deposit and distribution...

...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.      Additional... Today on the Addgene Podcast, we have another interview conducted by European Outreach Scientist Benoit...creation of improved fluorescent proteins through mutagenesis. Chapter 6 - The future of FRET and fluorescent...Amsterdam). He develops, characterizes and uses genetically encoded fluorescent probes. You can follow him...Additional Resources from the Addgene Blog Visit our Fluorescent Proteins featured topic page Read Joachim's...Subcellular Structures and Organelles Resources at Addgene.org Find Fluorescent Protein Plasmids Find Plasmids... addgene podcast...

...Addgene is excited to announce we now offer an export license application for rabies materials! Some ... export-controlled materials in the USA, where Addgene is located.  The export license, if granted, allows...ship rabies plasmids and viral vectors containing genetic elements from rabies plasmids to international ... export control license, once approved, allows Addgene to ship plasmids or viral vectors containing sequences...project when purchasing export-controlled materials. Addgene will then apply for the license for distribution...processing costs. Once the application is approved, Addgene then ships the material following our normal process... If you have any questions about this or other Addgene shipping and ordering processes, don’t hesitate...

...resources on addgene.org  Antibody GuideRecombinant antibodies available from AddgeneBrowse our recombinant...I love Addgene's new Antibody Guide and not just because I helped create it. But while antibodies, as...space to give proper credit to. Thankfully, as Addgene's resident blog editor, I could take the needed ...alternative reagents are created in the lab, instead of generated in vivo, they have higher reproducibility and ...small enough to be produced in bacteria and are generally cheaper to make than antibodies. Minibodies are...Designed ankyrin repeat proteins, or DARPins, are genetically engineered small affinity proteins, around 14...instead of the antibody constant region. They are generated using combinatorial libraries, and high specificity...