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We narrowed to 803 results for: Cre

Showing: 631 - 640 of 803 results
  1. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    Published
    Oct. 20, 2016, 2:30 p.m.
    ...trolling: The art of deliberately, cleverly, and secretly pissing people off, usually via the internet, ...excellent. Finally, as I said in the AMA, it’s incredibly important to find good mentors and incentivize...
  2. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...line you will need to grow this initial stock and create a set of vials of ‘Master’ stock, which can be ...thawed, it is time to reorder this cell line to create new Master and Working stocks to work from. The...
  3. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ...subtle and may include reduced saturation density, decreased rates of growth, or clumping. To ensure the best...Viral Vector Elements Learn How to Do Genome-Wide Screens with CRISPR Lentiviral Vectors Additional Resources...
  4. Plasmids 101: Control Plasmids

    Type
    Blog Post
    Published
    April 9, 2015, 3:29 p.m.
    ...Plasmid A is the decreased expression of Gene X. Ergo the positive control(s) should decrease the expression...
  5. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    Published
    April 3, 2014, 8:05 p.m.
    ...interest is toxic. To combat this, scientists have created synthetic promoters, which typically include some...with somewhat weak expression. lacIq mutation increases expression of the repressor 10x, thus tightening...
  6. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    Published
    Aug. 23, 2018, 12:05 p.m.
    ... S65T) in the original jellyfish GFP (AvGFP) to create enhanced GFP (EGFP), a variant with improved brightness...fluorescent probes, recent engineering efforts have increased the brightness of these genetically encoded light...
  7. Hot Plasmids: Summer 2024

    Type
    Blog Post
    Published
    Aug. 27, 2024, 1:15 p.m.
    ...A., Tao, Y. A., Duby, J. E., Steinbeck, B. J., McCreary, J., Pierce, S. E., … & Liu, D. R. (2024). Efficient...variety of DNA-targeting proteins, so the team created versions using dCas9, zinc finger proteins, and...
  8. Tips for Getting a Faculty Position

    Type
    Blog Post
    Published
    July 20, 2023, 1:15 p.m.
    ...completing the research will advance the field, create a new field, impact disease treatment, etc., again... His interests include the quality control of secretory proteins in the Endoplasmic Reticulum, optimization...
  9. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    Published
    Nov. 16, 2021, 2:15 p.m.
    ...immunofluorescence and indirect immunofluorescence work. Image created with BioRender.com   Indirect IF is a much ...with key considerations about each step. Image created with BioRender.com   The first couple of steps...
Showing: 631 - 640 of 803 results