We narrowed to 803 results for: Cre

...trolling: The art of deliberately, cleverly, and secretly pissing people off, usually via the internet, ...excellent. Finally, as I said in the AMA, it’s incredibly important to find good mentors and incentivize...

...line you will need to grow this initial stock and create a set of vials of ‘Master’ stock, which can be ...thawed, it is time to reorder this cell line to create new Master and Working stocks to work from. The...

...subtle and may include reduced saturation density, decreased rates of growth, or clumping. To ensure the best...Viral Vector Elements Learn How to Do Genome-Wide Screens with CRISPR Lentiviral Vectors Additional Resources...

...Plasmid A is the decreased expression of Gene X. Ergo the positive control(s) should decrease the expression...

...interest is toxic. To combat this, scientists have created synthetic promoters, which typically include some...with somewhat weak expression. lacIq mutation increases expression of the repressor 10x, thus tightening...

... S65T) in the original jellyfish GFP (AvGFP) to create enhanced GFP (EGFP), a variant with improved brightness...fluorescent probes, recent engineering efforts have increased the brightness of these genetically encoded light...

...A., Tao, Y. A., Duby, J. E., Steinbeck, B. J., McCreary, J., Pierce, S. E., … & Liu, D. R. (2024). Efficient...variety of DNA-targeting proteins, so the team created versions using dCas9, zinc finger proteins, and...

...plus one additional split vector strategy for increasing the size of the AAV donor template for CRISPR-mediated... AAV vector approaches Split vector approaches increase the size of the gene delivered by splitting the...

...completing the research will advance the field, create a new field, impact disease treatment, etc., again... His interests include the quality control of secretory proteins in the Endoplasmic Reticulum, optimization...

...immunofluorescence and indirect immunofluorescence work. Image created with BioRender.com   Indirect IF is a much ...with key considerations about each step. Image created with BioRender.com   The first couple of steps...