We narrowed to 790 results for: Cre

1. Antibodies 101: The Basics of Western Blotting

   Type

   Blog Post

   Published

   March 8, 2021, 2:41 p.m.

   ...proteins, use a higher percentage of acrylamide to increase migration resistance and improve separation. For...enhance resolution. In this setup, gels have an increasing range of acrylamide content from top to bottom...

2. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

   Type

   Blog Post

   Published

   June 28, 2018, 3:01 p.m.

   ...antibody” can be used for downstream IP/ChIP assays. Creating epitope-tagged clonal cell lines remains a challenge...crRNA complementary to the template strand could increase knock-in efficiency.Note: Our TAG-IN tool can ...

3. A Needle in a Base-Stack: Cas9 Structural Biology

   Type

   Blog Post

   Published

   June 4, 2024, 1:15 p.m.

   ..., while REC includes most of the bridge helix. Created with BioRender.com.   Cas9 has two major lobes...guide RNA, dark blue; DNA, gray; PAM site, yellow. Created with BioRender.com.   The exact conformational...

4. How to Deposit Your Plasmids with Addgene

   Type

   Blog Post

   Published

   Feb. 27, 2024, 2:15 p.m.

   ...process. To deposit plasmids online, you will need to create an account with Addgene before starting the process...plasmids, and adding all the plasmids at once increases the likelihood that the plasmids are given sequential...

5. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

   Type

   Blog Post

   Published

   Dec. 20, 2017, 2:33 p.m.

   ... cancer and your continued work is necessary to create therapeutics to cure the cancer. Authorship:  Publishing...translations of documents that are not in English. Create a Google Scholar account Sanitize your LinkedIn...

6. 'Tis the Season to #DeckTheLab

   Type

   Blog Post

   Published

   Dec. 2, 2016, 3:30 p.m.

   ...impressive entries, but we have faith our community of creative scientists will deliver some fabulous photos again...

7. CRISPR Guide Update

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   Blog Post

   Published

   Oct. 10, 2024, 1:15 p.m.

   ...added new sections to share and describe some incredible new CRISPR technologies developed and widely ...

8. Addgene's 20th Anniversary Party

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   Blog Post

   Published

   June 11, 2024, 1:15 p.m.

   ...can't celebrate Addgene without recognizing our incredible depositors and requestors. Thank you all so much...

9. The 12 Days of CRISPR

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   Blog Post

   Published

   Jan. 3, 2023, 2:15 p.m.

   ...gave to me: CRISPR activation and interference screens for primary human T cells🎵 On the 3rd day of CRISPR...

10. We’re Going Viral This Week!

    Type

    Blog Post

    Published

    Oct. 14, 2019, 1 p.m.

    ...came out last year on sucrose supplementation to increase AAV2 yields and we’re eager to share more! Lastly...