We narrowed to 939 results for: Gene

... KillerRed, a genetically encoded red fluorescent photosensitizer protein that generates ROS when illuminated...16369538. Additional Resources on the Addgene Blog Read Other Optogenetics Blog Posts Sign Up to Receive the...residues. Compared to MG, MG-2I has increased ROS generation when bound by the dL5** FAP.  The iodine-modifications...the main advantages of MG-2I-dL5** over other genetically encoded photosensitizers is that it works with...cells.  He et al also show that MG-2I-dL5** only generates small amounts of non-specific ROS when exposed...Watkins, S. C., . . . Bruchez, M. P. (2016). A genetically targetable near-infrared photosensitizer. Nature...Chepurnykh, T. V., . . . Lukyanov, K. A. (2005). A genetically encoded photosensitizer. Nature Biotechnology...

...Read Other Inside Addgene Posts Resources on the Addgene Website Meet the Addgene Management Team Join...Additional Resources on the Addgene Blog Get Management Advice from Addgene Executive Director Joanne ...meetings. Make sure to keep them manageable. At Addgene, teams can add as many points as they like to the...the meeting, take notes on the agenda points. At Addgene, we like to do this live on the screen using a ...review or project plans, before the meeting. At Addgene, we like to send around a Google doc where people...provided some tips above for what works well at Addgene, but meetings are never one-size-fits all. Feel...Join the Addgene Community ...

...commercialization. Many of the genetic pieces developed over the years were generated by large agricultural companies...you have developed a genetic tool for plant engineering, please share it with Addgene to both build the ...transformation to testing and a lack of well-characterized genetic tools make it challenging to engineer a specific... biotechnologists face is public perception of genetic modification (GM) and synthetic biology. The mistrust...research – this comprehensive understanding of the genetic pieces underpinning these mechanisms – our project...limited distribution. We hope that will change with Addgene’s expansion into plant synthetic biology. There ...and we are excited to see how this exchange of genetic tools facilitates plant science. Challenge #3: ...

...Fragments generation After you’ve designed your cloning scheme, you’re now ready to generate fragments...Paolo Colombi, a product development scientist at Addgene. Cloning can be quite an arduous process. The PCR...fragments. You can generate the parts of DNA that you want to assemble together in a combination of different...conditions. Purifying DNA fragments Once you’ve generated your DNA fragments, it is always a good habit ...step has to be strategy specific, there are few general parameters that you can adjust: temperature and...and time of incubation, and amount of DNA. As a general rule, try to use an excess of insert compared to...currently the product development scientist at Addgene. In the lab he develops new assays and processes...

...Plasmids 101 eBook Resources on Addgene.org Join the community of Addgene depositors here Check out our...repeating before you can finally dive deep into generating the data you’re excited about. When we were ...intervening five years, inflation has occurred. Here at Addgene, the consumables cost per stab has increased 50%...plasmids. Use repositories Repositories such as Addgene help share materials quickly and cheaply. We save...last minute experiments or changes. Fun fact: Addgene’s high-throughput approach means we virtually always...community engagement with and use of repositories like Addgene provides large-scale savings. How large? Using ...average of 5 hours of hands-on time per plasmid, Addgene, since its founding in 2004, has saved the scientific...

...Resources at Addgene Find the ClonTracer Library at Addgene Find Lentiviral Plasmids at Addgene Check out...abundance and clonal dynamics over time using next generation sequencing (NGS). The library was developed when... al., 2016).  Bhang deposited the library with Addgene because she wanted to make it as easy as possible...and she is excited to deposit the library with Addgene because it is a “really great way of broadly sharing...sharing these kinds of reagents.” Here at Addgene, we’re excited to see the creative new ways you put ...and for providing this powerful resource to the Addgene community! Carrie Bhang is a research investigator...in applying what we have learned about tumor heterogeneity to drug discovery and the development of combination...

...fit in a freezer). Our Addgene celebration was so much fun! Here's the Addgene Leadership team in all ... a million plasmids would fill? Two weeks ago, Addgene edged passed our one million plasmids shared milestone...might have you wondering how many plasmids leave Addgene headquarters every day. That would be an average...our shipping room above), but as Joanne Kamens, Addgene’s executive director, said during our celebratory...it takes to make one plasmid and how much time Addgene has saved researchers. Assuming 5 hours of hands-on...Addgenies on social media or around the office. @Addgene congrats for 1 million orders. @RothLabUNC accounts... — BryanRoth (@zenbrainest) August 21, 2018 As Addgene grows, we have been able to adapt to the needs ...

...proteins for optogenetic control of CRISPR-Cas9. By combining AcrIIA4, a Streptococcus pyogenes Cas9 inhibitor... a few of the new CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR...adapted for detecting SNPs. Learn more from this Addgene blog post Read the paper in Science Find the...the Cas14 plasmids Anti-CRISPR proteins for optogenetic control of CRISPR-Cas9 Anti-CRISPR proteins that...editing is now possible. Learn more from this Addgene blog post Read the paper in Nature Methods Find...a new CRISPR tool you’ve recently deposited to Addgene and you’d like it to be included in the next What...please let us know! Additional resources on the Addgene blog Download the CRISPR 101 eBook Find our CRISPR...

... plasmids for generating your own libraries are also available in the Sybody Generation Toolbox plasmid...lab found a way around this by using plasmids to generate sybodies, synthetic nanobodies against the spike...blog post.  A rapid selection platform for generating sybodies against membrane proteins Developing...membrane protein of interest using the Sybody Generation Toolbox and commercially available reagents in...libraries (Zimmermann et al., 2018). Find the Sybody Generation Toolbox! Starting with libraries and a biotinylated...relevant plasmids are available in the Sybody Generation Toolbox kit. Find the Seeger lab's SARS-CoV-2... S, Dawson RJP, Geertsma ER, Seeger MA (2020) Generation of synthetic nanobodies against delicate proteins...

... Figure 1: Discussing next-generation sequencing at the Addgene bioinformatics journal club! ...and level of general busy-ness will most likely the primary drivers of frequency. At Addgene, we opted to...recurring journal clubs on Immunology and Microbial Pathogenesis that occurred once a week for several months...want to pick a topic and have no one show up. At Addgene, we wanted to think more closely about the wealth...wealth of high-throughput sequencing (HTS) data we generate and maintain. It became clear that while several...messaging but we as scientists often prefer data. At Addgene we rely on polls and surveys. A mix of closed (...wandering monologue?  Assuming the feedback is generally positive and the meeting seemed to be of use, ...