...plasmid-based antibodies Resources on Addgene.org Discover Addgene’s antibody plasmid collection ...antibodies are produced Polyclonal antibodies are a heterogeneous mixture of many antibodies that recognize the... response.   Polyclonal antibodies are generated by injecting an animal with an immunogen, then...weeks later. Created with biorender.com. To generate polyclonal antibodies, an animal (such as a rabbit...might be the full-length protein that you want to generate antibodies against, or a unique peptide sequence...experiments to be aware of signal strength. In general, polyclonal antibodies produce a higher level of...the same species your polyclonal antibody was generated in or a polyclonal isotype control, to account...

...breaking into more audio and video on the Addgene Blog and Addgene website. As we push forward with these...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...present plasmid info with a new segment on the Addgene podcast - The Hot Plasmids Segment. Click on the...Listen to the entire Hot Plasmids episode! We generally publish 5-10 Hot Plasmid articles with each of...Additional Resources on the Addgene Blog Listen to Our Podcast Interview with Eye Researcher Connie Cepko...Science Videos from Tom McFadden Resources at Addgene.org Sign up to Receive Our Newsletter Check Out ...

...soon with some brand-new Addgene swag!   Last month, we introduced you to Blugene and Aavery's newest friend...friend, our Addgene antibody mascot, here to help us with our upcoming affinity reagent distribution launch...this form or head over to Twitter to vote on @addgene's Twitter poll. Voting is open until Feb. 22, 2022...winning name on Feb. 24. Stay tuned!    Original Blugene and Aavery would like your attention, please, as...that has led them to their biggest discovery yet: Addgene will soon be distributing affinity reagents. Name...hashtag #AntibodyMakesThree on Twitter. As with Blugene and Aavery, we’re looking for a gender neutral ... four will receive some rockin’ newly designed Addgene swag. Good luck and happy naming!   Fig....

...the blog Resources on Addgene.org Find fluorescent protein plasmids at Addgene Find chromoprotein plasmids...a redo :-( @SandTBioSci #CompetitiveStreaking @Addgene pic.twitter.com/mk44J4Gcj9 — Dave W will get vaccinated...found the chromoprotein plasmids available from Addgene, he knew it would be a perfect fit for the streak...biology. “What I'd like to do with the plasmids from Addgene is have the students transform them into different...transformation experiment, he plans to show how different genetic backgrounds of the host strains affect transformation... Youtube:     Additional resources on the Addgene blog Read our other blog posts on fluorescent ...plasmids at Addgene Check out additional written and video protocols ...

...AAV9 Optogenetics Adesnik New viral vector pAAV-nEF-Con/Foff DREADD Gi-mCherry AAV8 Chemogenetics Deisseroth...latest in Addgene's viral vectors services and resources!   More resources on the Addgene blog Addgene's...What's new in Addgene's ready-to-use viral vectors repository? Quite a bit! In this post, we'll share...Table 1: New ready-to-use viral vectors in the Addgene repository  Name Serotype Category Depositor...the full viral services collection or check out Addgene's Data Hub to view viral vector reports from other...

... Brian O'Neill.  More resources on the Addgene blog Addgene's Data Hub What is a Virus (and a Viral Vector...Addgene’s viral vector services allow scientists to conduct experiments faster, skipping the preparation...Table 1: New ready-to-use viral vectors in the Addgene repository  Name Serotype Category Depositor...serotype pAAV_hSyn-PdCO-mScarlet-WPRE AAV1 Optogenetics Ofer Yizhar New viral tool pAAV-Ef1a-fDIO...pAAV-Ef1a-fDIO hChR2(H134R)-EYFP AAVrg Optogenetics Karl Deisseroth New viral tool AiP13987: pAAV-AiE0452h_3xC2...different serotypes. Not sure what to request? Addgene's Data Hub can help, with over 100 user-submitted... the link below to keep up with the latest in Addgene's viral vector services and resources!   This blog...

... Ligands GCE4All: Making Genetic Code Expansion Accessible Addgene's Genetic Code Expansion Collection... & Small Molecule Fluorophores. Addgene Blog. https://blog.addgene.org/better-dyeing-through-chemistry-small-molecule-fluorophores... is now the most popular self-labeling tag in Addgene’s collection. CLIP-tag: Also in 2008, Kai Johnsson...specific with a small and minimally-disruptive genetic tag on the protein of interest. Table 1: Summary...are the most popular self-labeling enzymes in Addgene’s collection, so you may be able to get a plasmid... Pick, H., Vogel, H., & Johnsson, K. (2003). A general method for the covalent labeling of fusion proteins...10.1021/acs.biochem.1c00258 More resources from Addgene Better Dyeing Through Chemistry Illuminating Choices...

...shipping look like at Addgene? Here's an inside peak into the daily workings in Addgene's lab. We ship individual... all drive Addgene to succeed and to improve, so thank you. 500,000x Thank You! Addgene's new location...Yesterday we hit a huge milestone here at Addgene – 500,000 plasmids shipped! That's a 1/2 million plasmids...with shipping labels and paperwork. Our stacks of Addgene boxes are now ready to be sent around the world...distributed plasmids as of March 26, 2015. How does Addgene make it happen? Our awesome team of Addgenies! ...Cambridge, MA 02139 Have a question? Email: help@addgene.org Call: +1 (617) 225-9000         ...

...fresh columns, regenerated columns had similar DNA yields. Additionally, columns regenerated up to five times...DNA This kit-free plasmid miniprep protocol from Addgene follows a similar workflow as a column-based plasmid...cellulose dipstick. Image: Zou et al., 2018. Regeneration of silica columns for DNA purification Starting... and Deng labs developed a simple method for regenerating silica columns that come with PCR product purification...before a wash with ddH2O and then ethanol. After regeneration, trace amounts of DNA (3 femtograms/ul) could...in the comments! Additional resources on the Addgene blog Read our Plasmids 101 blog series Find other...our plasmid cloning blog posts Resources on Addgene.org Find protocols for molecular biology and cloning...

...resources on addgene.org Addgene's Antibody Guide Addgene's Ready-to-Use Antibody collection Addgene's Recombinant...create and use a standard curve and provide some general considerations for scientists planning to use them...for the known concentrations (standard curve), generating an equation that allows you to determine the ... triplicate. The closeness of the data points generated by replicate values provides useful information.... With the help of graphing software, you then generate a trendline for the data. The appropriate trendline...R2 never dip below 0.95! More resources on the Addgene blog Antibodies 101: ELISAs The Four ELISAs and...