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We narrowed to 892 results for: CAN

Showing: 641 - 650 of 892 results

  1. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    Published
    April 6, 2021, 12:30 p.m.
    ...into a single FASTA sequence that our QC scientists can easily analyze (see below for what we look out for...The full details of that workflow and its analysis can be found here: A Novel Next-Generation Sequencing... library in question. We will order primers that can amplify the insert of the library. This PCR step ...sequencing as well as unique barcodes per sample so we can assign sequencing reads to the correct sample. The...Addgene, each part of this new process involves a significant amount of QC. First, we perform Picogreen quantification...

  2. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ...understand the power of a physical model. Until then, you can only wonder what I am talking about. How do researchers...   Seeing is believing. And in that sense, models can be both useful, and dangerous. Subscribe to CRISPR...we have when we jump into a new modeling project can be a dangerous thing. Fortunately, Jacob was able...physical model is an effective teaching tool. You can read about Jacob’s initial reaction to this model...flexibility that the Cas9 protein must have in order to scan along the double-stranded DNA, recognize a PAM sequence...

  3. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    Published
    Sept. 17, 2015, 2:30 p.m.
    ...Pulse Stimulator, which can control output of several lasers simultaneously, can be used to set the laser...to use to determine the ideal excitation). Lasers can be purchased through Laserglow, which caters to optogenetics.... However, an important caveat is that one laser can stimulate only a single animal during the course ...cement (a similar procedure as implanting a guide cannula). A fiber optic patch cable is then connected from...portion of optogenetics (though it does require a significant amount of equipment, most of which is relatively...point; the fiber optics need to be polished to significant smoothness to prevent drastic loss of efficiency...stimulation, the rate of movement of the animal significantly increases. Before you get your experiment started...

  4. Addgene’s GIPHY Page is Here to Help You Express Yourself (and Your Gene of Interest)

    Type
    Blog Post
    Published
    Aug. 17, 2021, 1:15 p.m.
    ...is, we’re not only sharing them with you, but you can share them with your coworkers, labmates, and friends...more than plasmids these days. via GIPHY   You can see for yourself at giphy.com/addgene. But that’s... interest).   Blugene is so excited they can’t stop dancing! via GIPHY   Don’t forget to check...

  5. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type
    Blog Post
    Published
    Nov. 6, 2018, 1:12 p.m.
    ... for these methods can be found on Addgene’s stem cell page. On this page, you can also find a table with...vitro is known as reprogramming [1]. The process can be divided into two stages: Dedifferentiation - ...cell type of choice. The four reprogramming factors can be delivered and expressed in multiple somatic cells... of somatic cells. The goal is to use cells that can be easily isolated from donors. Apart from fibroblasts...Otic hair cells [31] Neurons [32, 33, 34] You can find a variety of plasmids for differentiation here...the Stem Cell Field Learn how lentiviral vectors can be used for gene delivery Resources on Addgene.org...pluripotent stem cells via temporal modulation of canonical Wnt signaling. Proc Natl Acad Sci U S A, 2012....

  6. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

    Type
    Blog Post
    Published
    Dec. 20, 2017, 2:33 p.m.
    ...last year less than 30% of applicants received sponsorship) then he or she can start working in October ...this case.  You can still assist your PI in the peer review process, but remember you cannot use it as documentary...responsibilities during their careers - being aware of this can help take the pressure off having to find the perfect...process or quota like industry, these organizations can be a good choice for individuals needing sponsorship...organization – this is not referring to one that you can pay for, but one that you are invited to join (selected...attractive option.    Your Expert Letters of support can mean that your case gets approved, denied or a Request...who has cited your work. Immediate steps that you can take to prepare for the green card process Update...

  7. Safe Port in a Storm...How Addgene is Weathering the Pandemic

    Type
    Blog Post
    Published
    Sept. 17, 2020, 1:15 p.m.
    ...buffeted by wind and waves and sometimes wonder if we can take another blow. Back in March, we never expected... when we need as much open science sharing as we can get. The effects of Addgene’s brief shut-down...excellence through whatever lies ahead this year. We can see signs of the storm passing. We’ve recently piloted...

  8. Arabidopsis in Education: How the Arabidopsis Biological Resource Center Brings Plant Science to Life

    Type
    Blog Post
    Published
    April 12, 2018, 1:02 p.m.
    ...research to explore a variety of phenomena, and so too can it be used to teach a variety of science concepts...deeper understanding of scientific concepts than can be gained from lectures alone. Using plants as an...Resources on the Addgene Blog Learn how BioBuilder can bring Synthetic Biology into the classroom Engineering...Arabidopsis in the classroom With funding from the American Society of Plant Biologists and support from the...

  9. Sensing Neuronal Dopamine

    Type
    Blog Post
    Published
    July 30, 2019, 12:59 p.m.
    ... fire at a certain frequency, and this frequency can indicate behavioral state, for example, if someone...quantitation. The advantage of this method is that it can be performed in a live awake animal by surgical implantation...offers immediate measurements in a live animal that can be correlated to behavior, the technique is relatively...

  10. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    Published
    Sept. 14, 2017, 12:40 p.m.
    ...interesting question: why engineer a new Cas9 when you can just find one that naturally occurs? This is different...functions at temperatures much warmer than SpCas9 can tolerate. Harrington et al tested GeoCas9’s love ...cells Besides being able to take some heat, GeoCas9 can introduce genomic edits in mammalian cells. Harrington...
Showing: 641 - 650 of 892 results