We narrowed to 904 results for: CAN

... particular disease, cell type or drug treatment can browse through screens performed in relevant cell...otherwise be hard to identify. Figure 2: Users can cross-interrogate screens from the literature for...original publication via its PubMed identifier, which can also be used to search the entire CRISPR database... the term "breast cancer" returns 40 screens performed in 26 different breast cancer cell lines. Screenshot...The analytical method for score assignment and significance thresholds used by the authors to determine ...

...planned. Blugene also has full arms so that they can hold and hug items closer and do so more animatedly...with a variety of science-inspired friends. As you can see on the homepage, Blugene is now accompanied by... viral vector for your next experiment, that you can find what you need and feel happy and inspired to...

...deposited in Addgene!” As you can imagine, answering plasmid requests can be a time-consuming chore for...benefits of sharing plasmids with us. One way we can gauge if people are happy depositors is if they deposit...

...diverse and exciting field and to find out how we can serve them better. Here’s a little bit of what we...into science education and outreach ourselves, we can a learn a lot from these ASM pros and hope to leverage...materials! Resources on the Addgene Blog Learn How You Can Use Addgene's Microbiology Pages Read Our Post on...page and, just a few weeks ago, we attended the American Society for Microbiology's annual meeting (ASM...

...made the accidental discovery that laser treatment can photoconvert GFP from green to RED! This simple technique...photoconvertible, new studies of spatiotemporal dynamics can be undertaken using available GFP lines. Like many...compartments. Sattarzadeh et al. have shown that GFP can be rapidly and efficiently photoconverted in plant...

...Cre-estrogen receptor ligand binding domain-fusion can prevent this toxicity, but with the caveat of partial...levels. These data show that multiple recombinases can be used together to designate specific patterns of...shows that the yeast recombinases KD, B2, B3 and FLP can be used together in Drosophila as a toolkit for specific...

... demonstrated in their PNAS paper that SpyLigase can be used to link affibodies or antibodies against ...13 amino acids, but SpyCatcher is a protein. You can fuse SpyCatcher onto things, but even better if one...larger protein assemblies? Howarth: Peptide tags can be very generic; you should be able to use the same...system.) Cancer and other applications Addgene: What does this technology mean in the context of cancer? Howarth...ultimate goal to improve the diagnosis of disease, and cancer in particular. They recently introduced the SpyTag... common tumor markers to subsequently capture cancerous cells expressing low levels of tumor antigen. ...big impact on our understanding and treatment of cancer. It is clear that one needs to capture a diverse...

... hopefully make your life a little easier. These can range from technical tips as in our episode on Barcoding...machine. Lab tip #1: Glove trick Putting on lab gloves can sometimes be annoying. To make it easier, try this...Lab tip #3: Barcode everything Use barcodes and scanners to keep track of reagents in your lab. With barcode...

...understand the power of a physical model. Until then, you can only wonder what I am talking about. How do researchers...   Seeing is believing. And in that sense, models can be both useful, and dangerous. Subscribe to CRISPR...we have when we jump into a new modeling project can be a dangerous thing. Fortunately, Jacob was able...physical model is an effective teaching tool. You can read about Jacob’s initial reaction to this model...flexibility that the Cas9 protein must have in order to scan along the double-stranded DNA, recognize a PAM sequence...

...public. You can find out more about Clare at the Biology Dept. site.  References 1. American Association... mutations accumulated over time? Undergraduates can help to answer this question! The Pathways Over ...deletion). If the phenotype is corrected, students can be relatively confident that the gene from the test...epitope tags to the C-termini of Met proteins, which can be used to confirm protein expression by western ...undergraduate students (1, 2), but educators face significant challenges in designing suitable projects. In...cerevisiae deletion strains are ∆ura3 mutants and cannot grow without uracil supplementation (6). Expression... Students who participate in the course show significant gains in both content knowledge and research ...