We narrowed to 892 results for: CAN
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TypeBlog PostPublishedJuly 21, 2022, 1:15 p.m....from tertiary study, the myriad trajectories ahead can be condensed into two discernible paths. The first...how to utilize a wide array of media in doing so—can prove invaluable in the pursuit of public engagement... misinformation in the wake of a global pandemic can make for a sore reminder of how far we have yet to...
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Addgene Begins Distribution of Recombinant Antibodies
TypeBlog PostPublishedMarch 28, 2022, 4:30 p.m....immunofluorescence, conjugates, isotypes, and more. How You Can Participate Addgene will continue to add new antibodies...for advancing science. Here are some ways that you can participate: If you make recombinant antibodies ...easily share plasmids with each other. Together, we can transform the antibody sharing landscape as well.... -
Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring
TypeBlog PostPublishedNov. 12, 2019, 2 p.m....IgnaviCas9 here! What can you do with IgnaviCas9? Why would a thermostable Cas9 be useful? It can enable applications...nuclease activity is desired and time or protocol steps can be saved by flexibly deploying IgnaviCas9 without... -
Donations from Addgene to Yield Answers for Rare Disease Researchers
TypeBlog PostPublishedSept. 15, 2015, 2:30 p.m....help us acquire a lot of those plasmids so that we can try many different things in parallel.” Alternating...painful and accompanied by migraines. Those attacks can be brought on by a number of stressors, including...work on Waardenburg syndrome, a rare disease that can cause hearing loss and changes in coloring of the...prone to developing metastatic melanomas and other cancers. Joshua Wang (bottom right), also of the Wistar... -
Management for Scientists: Giving Feedback
TypeBlog PostPublishedFeb. 6, 2015, 2:46 p.m.... writing down specific examples and notes so you can get back to the issues if the conversation gets off...possible. If you wait too long, a small correction can become an unnecessarily big issue for you and for...What could I (manager) have done differently? What Can You Learn Going Forward: If you could plan this ... -
10 Ways to Share Your Science!
TypeBlog PostPublishedMay 13, 2014, 8:34 p.m....time to hone your communication skills. So, where can a graduate student or post-doc start? The good news...your science with the community. Some of them you can do today! 10 ways to share your science 1) If you...consider how those stories are being told. Twitter can be a good way to follow news about particular subjects... -
Find and Share AAV Data with Addgene's New AAV Data Hub
TypeBlog PostPublishedOct. 8, 2019, 12:33 p.m....Every entry has an accompanying image so that you can see for yourself how the AAV performed in that experimental...we hope to mine the data to find patterns that we can share with you about promoters, serotypes, expression... -
An Integrin Antibody Toolkit from IPI
TypeBlog PostPublishedMarch 28, 2024, 1:15 p.m....interact with extracellular ligands, their ectodomains can extend and open, binding ligands with high affinity...function. With the right research reagents, researchers can measure this conformational change to investigate... cellular processes. Figure 1: Integrins can shape into three conformations: (A) bent-closed, ...its open state, activating the receptor. Ligands can bind to integrins in their closed states (A) and ...laboratory techniques related to antibody discovery. You can find cell surface receptor biology talks on the IPI...kidney development. Despite their ubiquity and significance, these receptors have proved challenging to ... -
Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)
TypeBlog PostPublishedMarch 7, 2017, 3:30 p.m....Sometimes the PCR products can look smeared, so having an undigested control can help distinguish between.... Cas9 delivery Overview: Before any editing can begin we need our A549 cells to express Cas9. In ...Addgene viruses are provided at a specific titer, you can better plan your transductions and use a range of...well dish. My protocol with many additional tips can be found here. Figure 3: A surprise for the author...gene. I used existing BRAF gRNA viruses, but you can find a list of validated gRNA plasmids on our website... implicated in several human cancers) in A549 cells (a human lung cancer cell line), armed only with viruses... CRISPR, I was able to knockout BRAF in a human cancer cell line in about 1 month, which I’d say is pretty... -
With an Eye Towards the Future, We Look Back at the March for Science
TypeBlog PostPublishedOct. 24, 2017, 1:54 p.m.... as often as they can. Karissa Milbury is currently a PhD candidate at the BC Cancer Agency and University... April 22, 2017. While experiments (and editors) can move slowly, news about possible broad changes to...also studies energy markets in his spare time. You can follow him on twitter at @thompn4. New York City,...stars and the instruments we use to study them. You can follow her at alexandrazgreenbaum.wordpress.com or...supporters. I'm raising some little scientists (as you can see in the picture) and this march proved to be the...Within the Sciences Learn How Changing Labor Laws Can Effect Postdoc Wages ... breast cancer at the Goodman Cancer Research Centre at McGill University in Montreal, Canada. Outside...