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...Addgene is excited to announce we now offer an export license application for rabies materials! Some ... export-controlled materials in the USA, where Addgene is located.  The export license, if granted, allows...ship rabies plasmids and viral vectors containing genetic elements from rabies plasmids to international ... export control license, once approved, allows Addgene to ship plasmids or viral vectors containing sequences...project when purchasing export-controlled materials. Addgene will then apply for the license for distribution...processing costs. Once the application is approved, Addgene then ships the material following our normal process... If you have any questions about this or other Addgene shipping and ordering processes, don’t hesitate...

...resources on addgene.org  Antibody GuideRecombinant antibodies available from AddgeneBrowse our recombinant...I love Addgene's new Antibody Guide and not just because I helped create it. But while antibodies, as...space to give proper credit to. Thankfully, as Addgene's resident blog editor, I could take the needed ...alternative reagents are created in the lab, instead of generated in vivo, they have higher reproducibility and ...small enough to be produced in bacteria and are generally cheaper to make than antibodies. Minibodies are...Designed ankyrin repeat proteins, or DARPins, are genetically engineered small affinity proteins, around 14...instead of the antibody constant region. They are generated using combinatorial libraries, and high specificity...

...plasmid-based antibodies Resources on Addgene.org Discover Addgene’s antibody plasmid collection ...antibodies are produced Polyclonal antibodies are a heterogeneous mixture of many antibodies that recognize the... response.   Polyclonal antibodies are generated by injecting an animal with an immunogen, then...weeks later. Created with biorender.com. To generate polyclonal antibodies, an animal (such as a rabbit...might be the full-length protein that you want to generate antibodies against, or a unique peptide sequence...experiments to be aware of signal strength. In general, polyclonal antibodies produce a higher level of...the same species your polyclonal antibody was generated in or a polyclonal isotype control, to account...

...breaking into more audio and video on the Addgene Blog and Addgene website. As we push forward with these...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...present plasmid info with a new segment on the Addgene podcast - The Hot Plasmids Segment. Click on the...Listen to the entire Hot Plasmids episode! We generally publish 5-10 Hot Plasmid articles with each of...Additional Resources on the Addgene Blog Listen to Our Podcast Interview with Eye Researcher Connie Cepko...Science Videos from Tom McFadden Resources at Addgene.org Sign up to Receive Our Newsletter Check Out ...

...soon with some brand-new Addgene swag!   Last month, we introduced you to Blugene and Aavery's newest friend...friend, our Addgene antibody mascot, here to help us with our upcoming affinity reagent distribution launch...this form or head over to Twitter to vote on @addgene's Twitter poll. Voting is open until Feb. 22, 2022...winning name on Feb. 24. Stay tuned!    Original Blugene and Aavery would like your attention, please, as...that has led them to their biggest discovery yet: Addgene will soon be distributing affinity reagents. Name...hashtag #AntibodyMakesThree on Twitter. As with Blugene and Aavery, we’re looking for a gender neutral ... four will receive some rockin’ newly designed Addgene swag. Good luck and happy naming!   Fig....

...the blog Resources on Addgene.org Find fluorescent protein plasmids at Addgene Find chromoprotein plasmids...a redo :-( @SandTBioSci #CompetitiveStreaking @Addgene pic.twitter.com/mk44J4Gcj9 — Dave W will get vaccinated...found the chromoprotein plasmids available from Addgene, he knew it would be a perfect fit for the streak...biology. “What I'd like to do with the plasmids from Addgene is have the students transform them into different...transformation experiment, he plans to show how different genetic backgrounds of the host strains affect transformation... Youtube:     Additional resources on the Addgene blog Read our other blog posts on fluorescent ...plasmids at Addgene Check out additional written and video protocols ...

... Brian O'Neill.  More resources on the Addgene blog Addgene's Data Hub What is a Virus (and a Viral Vector...Addgene’s viral vector services allow scientists to conduct experiments faster, skipping the preparation...Table 1: New ready-to-use viral vectors in the Addgene repository  Name Serotype Category Depositor...serotype pAAV_hSyn-PdCO-mScarlet-WPRE AAV1 Optogenetics Ofer Yizhar New viral tool pAAV-Ef1a-fDIO...pAAV-Ef1a-fDIO hChR2(H134R)-EYFP AAVrg Optogenetics Karl Deisseroth New viral tool AiP13987: pAAV-AiE0452h_3xC2...different serotypes. Not sure what to request? Addgene's Data Hub can help, with over 100 user-submitted... the link below to keep up with the latest in Addgene's viral vector services and resources!   This blog...

...AAV9 Optogenetics Adesnik New viral vector pAAV-nEF-Con/Foff DREADD Gi-mCherry AAV8 Chemogenetics Deisseroth...latest in Addgene's viral vectors services and resources!   More resources on the Addgene blog Addgene's...What's new in Addgene's ready-to-use viral vectors repository? Quite a bit! In this post, we'll share...Table 1: New ready-to-use viral vectors in the Addgene repository  Name Serotype Category Depositor...the full viral services collection or check out Addgene's Data Hub to view viral vector reports from other...

...resources on addgene.org Addgene's Antibody Guide Addgene's Ready-to-Use Antibody collection Addgene's Recombinant...create and use a standard curve and provide some general considerations for scientists planning to use them...for the known concentrations (standard curve), generating an equation that allows you to determine the ... triplicate. The closeness of the data points generated by replicate values provides useful information.... With the help of graphing software, you then generate a trendline for the data. The appropriate trendline...R2 never dip below 0.95! More resources on the Addgene blog Antibodies 101: ELISAs The Four ELISAs and...

...fresh columns, regenerated columns had similar DNA yields. Additionally, columns regenerated up to five times...DNA This kit-free plasmid miniprep protocol from Addgene follows a similar workflow as a column-based plasmid...cellulose dipstick. Image: Zou et al., 2018. Regeneration of silica columns for DNA purification Starting... and Deng labs developed a simple method for regenerating silica columns that come with PCR product purification...before a wash with ddH2O and then ethanol. After regeneration, trace amounts of DNA (3 femtograms/ul) could...in the comments! Additional resources on the Addgene blog Read our Plasmids 101 blog series Find other...our plasmid cloning blog posts Resources on Addgene.org Find protocols for molecular biology and cloning...