...Immunofluorescence Resources on Addgene.org Find recombinant antibodies available at Addgene Protocols for Immunocytochemistry...antibodies and appropriate secondary antibodies. The general process is similar to standard immunofluorescence...IHC validated or ICC validated antibodies from Addgene), or be prepared to run some extra controls and...quantify accurately, but can be especially helpful to generate more signal from low abundance targets. Analysis...you find yourself stuck with red-green images generated automatically from the microscope software, you...three targets or you’re pushing the limits like Addgene depositor Ronald Germain (Radtke et al., 2020 multiplexed...10.1073/pnas.2018488117 Additional Resources on the Addgene blog Introduction to Antibodies Which Fluorescence...

...study apoptosis in vivo, scientists have used genetically encoded fluorescent executioner caspase reporters...to issues with tissue autofluorescence, cell heterogeneity, and/or cell position changes (To et al., 2016...They then optimized the length of both linkers to generate a reporter that would fluoresce: FlipCherry. FlipCherry...array of diseases and pathologies such as neurodegenerative diseases, autoimmune disorders, and cancer...find the plasmids for FlipGFP and FlipCherry at Addgene. References Cabantous, Stéphanie, et al. "A new...PubMed PMID: 30821975. Additional resources on the Addgene blog Read our fluorescent proteins blog posts ...Read our plasmids 101 blog series Resources on Addgene.org Browse the fluorescent protein collection Find...

...exciting revelation. Science in any field can generally be applied to improve our lives and solve global...Typically has a Master's or Bachelor's degree Generally a short-term position (1-2 years) Often a gap-...who opted to join labs in cancer biology or neurodegenerative disease because they had close relatives suffering...that can make or break a lab experience. Previous Addgene blogs have discussed the process that goes into...also found that it is more common among first generation students, women, BIPOC, and other minoritized...academia or industry, so feel free to explore! Addgene has an entire page dedicated to different career... the Daily Psychological Experiences of First-Generation College Students. Social Psychological and Personality...

...Toolbox for quickly generating zebrafish transgenic lines to study neurodegenerative diseases. Listen to...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...Additional Resources on the Addgene Blog Listen to Our Podcast Interview with Eye Researcher Connie Cepko...Science Videos from Tom McFadden Resources at Addgene.org Sign up to Receive Our Newsletter Check Out ...

...resources on the Addgene blog Addgene Blog Biosensors posts Resources on Addgene.org Fluorescent Biosensors...where should you begin? Have no fear, Addgene is here to help!  General Design Figure 1: Two proteins...the FP back together (Figure 1). This method is generally known as Bimolecular Fluorescence Complementation...proteins in Caenorhabditis elegans. Goudeau et al. Genetics. 2021 Cynthia Kenyon mRuby4 mRuby4(1-10), pairs...

...disponible en español! Esta traducción fue regalada a Addgene por Daniel Mejías Sánchez, ¡y no podríamos estar...emocionados! Después de recibir la traducción, Addgene contrató a una traductora científica profesional...Descargar CRISPR 101: Una herramienta de edición Addgene tiene una gran audiencia internacional, incluido...available in Spanish!  This translation was gifted to Addgene by Daniel Mejías Sánchez, and we could not be more...more excited! After receiving the translation, Addgene contracted with a professional scientific translator...Download CRISPR 101: Una herramienta de edición  Addgene has a large international audience, including working...

...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.      Additional... In this episode of the Addgene Podcast, we introduce you to the Journal of Emerging Investigators, an...recently submitted to the journal with funding from Addgene. Listen to the interview with the Journal of Emerging...Additional Resources from the Addgene Blog Find education resources Use our Plasmids 101 eBook to teach ...genome enegineering technology Resources at Addgene.org Find basic molecular biology protocols Check...

...intern who shared their experience working at Addgene. An Addgene Summer Internship Matteo Tardelli, who ...contributing to the Addgene blog? We'd love to hear from you! Email us anytime at news@addgene.org. ... very thankful time of year for those of us at Addgene! And we can’t think of any better way to start ...to the blog. Thank you! More resources on the Addgene blog 10 Great Guest Posts We're Thankful For Lucky...

...molecular weight ladders were recently deposited with Addgene. A team of undergraduate students led by Dr. Song...restriction digested with PstI or EcoRV, these plasmids generate 100 bp or 1 kb DNA ladders, respectively. Unlike...pPSU1 and the pPSU2 plasmids are available from Addgene. Learn How to Analyze a Restriction Digest References.... L., & Tan, S. (2017). The pPSU Plasmids for Generating DNA Molecular Weight Markers. Scientific Reports...Central PMCID: 5446395. Additional Resources on the Addgene Blog Are you a Frugal Scientist? Save Money with...the Evolution of Lab Techniques Resources on Addgene.org Use the Kit-Free Alkaline Lysis Plasmid Miniprep...

...production requires animal sacrifice and precludes genetic engineering of a tag or fusion with a reporter ...described in Pleiner et al, the key steps for generating nanobodies are outlined below; no alpaca required...your work. Species Antibody Subclass Nanobody Addgene Plasmid Rabbit N/A* TP897 pTP1183 (1x cysteine...nanobodies from the column. bdNEDP1 protease can be generated using plasmid pDG02583. Label the Nanobodies: ...29263082 Find plasmids from this publication at Addgene. 2. Bates, M., Böhning, M.I., Chug, H., Görlich...PubMed PMID: 23495938. Additional Resources on the Addgene Blog DIY DNA ladders from Penn State University... protein expression Additional Resources on Addgene.org Find more nanobody expression plasmids here ...