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We narrowed to 892 results for: CAN

Showing: 681 - 690 of 892 results

  1. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

    Type
    Blog Post
    Published
    Oct. 11, 2018, 12:30 p.m.
    ...transformants. To compensate for this, researchers can plate larger volumes of the outgrowth containing ...ml outgrowth spread on each transformation plate can be manipulated to result in appropriate levels of...assembly, ligation of mismatched pairs of overhangs can lead to incorrect assemblies, so care must be taken...confirm the accuracy of assembly. The fidelity data can be applied to derive similar high-fidelity overhangs... by guest bloggers Becky Kucera, M.Sc. and Eric Cantor, Ph.D. from New England Biolabs. Golden gate assembly...-Crick base pair substrates, it demonstrates significant activity on some mismatch-containing pairings...our guest bloggers Becky Kucera, M.Sc. and Eric Cantor, Ph.D. from New England Biolabs. Becky Kucera is...

  2. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ...adeno-associated viral vectors can also target non-dividing cells, lentiviral vectors can carry much more genetic... often used as a substitute for HIV-1 Env, which can only infect CD4+ cells. 3rd generation lentiviral...Lentiviral vectors in the clinic Since lentiviral vectors can deliver a large amount of DNA (~8 kb) with a relatively...beta-thalassemia. Lentiviral vectors are promising agents for cancer immunotherapy, but this research is still in the...Central PMCID: PMC3355472. 8. Lentiviral vectors in cancer immunotherapy. Oldham RA, Berinstein EM, Medin ...

  3. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ...essential to most bacteria, custom CRISPR antimicrobials can target specific sequences in a single virulent bacterial...plasmids containing a phage origin of replication that can be packaged into replication-incompetent phage particles...Once these bacteria have been killed, antibiotics can be used to target the sensitive population. Yosef.... Future research will show if this new strategy can help us win back ground in the fight against bacterial...

  4. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    Published
    Aug. 20, 2015, 2:30 p.m.
    ...federal regulations. A grant professional’s job duties can range from coordinating the grant application and...completed. To enter and advance the profession, you can find professional development, certification, degrees...the grants profession? The four steps listed below can give you the resources and path to help you enter... first career, a mentor in the Grants Profession can help you focus your career goals, identify your niche...evaluates a candidate's expertise (a minimum of 3 years experience) and experience through the candidate application... the candidate’s ability to write a grant application through a written exam, and the candidate’s knowledge...grant seekers to use online applications with significant character limits for the written portion of the...

  5. PEI Calculator for Planning AAV Packaging Transfections

    Type
    Blog Post
    Published
    Jan. 23, 2018, 2:04 p.m.
    ... Preparation is a key step to any experiment and can help prevent future headaches. To help you plan PEI...you need to do a few calculations. This calculator can do the math for you and it’s simple to make adjustments...

  6. Countdown to Halloween @Addgene

    Type
    Blog Post
    Published
    Oct. 29, 2014, 5:26 p.m.
    ...there’s always nostalgia (old board games and movies can provide great character costumes). All of this costume... chose their favorite plasmid and replicated it. Can you guess each plasmid? Our lab paid tribute to ...Success is sweet – as sweet as all that Halloween candy you’ll gorge yourself on in the coming days. Hopefully...

  7. Addgene Depositors Get More Citations

    Type
    Blog Post
    Published
    April 20, 2017, 2:30 p.m.
    ...Addgene (Figure 1). Even aggregated this much, you can see the 2013 ‘bump’ related to the CRISPR/Cas papers...comprised 10 percent. Based on this new re-weighting, we can compare citations where an equal share of papers ...Microbiology  1.6% 1.2%  0.75   125.3 In figure 2, you can see the full impact from using these weighted numbers...journal. And so, while our quantitative analysis cannot rule out all other explanations, we do think that...used in this work. Samantha Zyontz is a Ph.D. candidate at the MIT Sloan School of Management. She is ...

  8. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    Published
    Aug. 23, 2022, 1:15 p.m.
    ...issues creates conditions under which misinformation can flourish. I’ve never forgot about all the anxious...helped run workshops where I showed people how they can make reusable cloth menstrual pads while we talked... the very least, believe – to be true so that we can tailor our communications to better meet the needs...controversial medical issues. Participants gave significantly more favourable ratings to the abstracts that...understand. Why should we trust something that we cannot read or verify for ourselves? Further, when science...

  9. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    Published
    April 4, 2023, 1 p.m.
    ...aerobe. It’s also a methylotropic yeast, meaning it can utilize methanol as its sole source of carbon and...industries as a protein expression workhorse. This yeast can also give more protein bang for your media buck -...media change into methanol and peroxisomal autophagy can be induced by removing methanol from the media. This...

  10. AAV Packaged on Request is Here!

    Type
    Blog Post
    Published
    Jan. 10, 2025, 1:01 p.m.
    ...service, has officially launched! As of today, you can request any of over 3,000 eligible AAV cargo plasmids...Packaged on Request With Packaged on Request, you can find your plasmids and your viral packaging service...
Showing: 681 - 690 of 892 results