We narrowed to 927 results for: Gene
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TypeBlog PostPublishedFeb. 14, 2019, 2:35 p.m....PMC5517107. Additional resources on the Addgene blog Subscribe to the Addgene blog Find lab tips for your research...familiar with the alarming amount of waste that is generated. This is a systemic problem that we need to address...to learn about practices I could bring both to Addgene, and share with my peers. In fact, there was so...account for an estimated 2% of global plastic generation. Less than 9% of plastics are ever recycled, ... about conservation in the lab Resources on Addgene.org Sign up for our quarterly newsletter Help other...resources by sharing plasmids Find plasmids at Addgene ...
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New and Upcoming Viral Vectors - December 2019
TypeBlog PostPublishedDec. 19, 2019, 2:40 p.m....include: Optogenetics AAV Control AAV Biosensor AAV And more! Optogenetics Optogenetics continues ...viral vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols...you pick the best AAV for your experiment. The Addgene viral service has now expanded to offer many of...pEF1a-DIO-FLPo-WPRE-hGHpA Additional resources on the Addgene blog Read all of our viral vectors blog posts ... -
Antibodies 101: Multiplex Immunofluorescence
TypeBlog PostPublishedMay 23, 2023, 1:15 p.m....Immunofluorescence Resources on Addgene.org Find recombinant antibodies available at Addgene Protocols for Immunocytochemistry...antibodies and appropriate secondary antibodies. The general process is similar to standard immunofluorescence...IHC validated or ICC validated antibodies from Addgene), or be prepared to run some extra controls and...quantify accurately, but can be especially helpful to generate more signal from low abundance targets. Analysis...you find yourself stuck with red-green images generated automatically from the microscope software, you...three targets or you’re pushing the limits like Addgene depositor Ronald Germain (Radtke et al., 2020 multiplexed...10.1073/pnas.2018488117 Additional Resources on the Addgene blog Introduction to Antibodies Which Fluorescence... -
FlipGFP, a novel fluorescence protease reporter to study apoptosis
TypeBlog PostPublishedMay 21, 2019, 12:10 p.m....study apoptosis in vivo, scientists have used genetically encoded fluorescent executioner caspase reporters...to issues with tissue autofluorescence, cell heterogeneity, and/or cell position changes (To et al., 2016...They then optimized the length of both linkers to generate a reporter that would fluoresce: FlipCherry. FlipCherry...array of diseases and pathologies such as neurodegenerative diseases, autoimmune disorders, and cancer...find the plasmids for FlipGFP and FlipCherry at Addgene. References Cabantous, Stéphanie, et al. "A new...PubMed PMID: 30821975. Additional resources on the Addgene blog Read our fluorescent proteins blog posts ...Read our plasmids 101 blog series Resources on Addgene.org Browse the fluorescent protein collection Find... -
A Guide to Getting Started in Undergrad Research
TypeBlog PostPublishedJune 8, 2021, 1:15 p.m....exciting revelation. Science in any field can generally be applied to improve our lives and solve global...Typically has a Master's or Bachelor's degree Generally a short-term position (1-2 years) Often a gap-...who opted to join labs in cancer biology or neurodegenerative disease because they had close relatives suffering...that can make or break a lab experience. Previous Addgene blogs have discussed the process that goes into...also found that it is more common among first generation students, women, BIPOC, and other minoritized...academia or industry, so feel free to explore! Addgene has an entire page dedicated to different career... the Daily Psychological Experiences of First-Generation College Students. Social Psychological and Personality... -
Split Fluorescent Proteins for Studying Protein-Protein Interactions
TypeBlog PostPublishedMay 3, 2022, 1:15 p.m....resources on the Addgene blog Addgene Blog Biosensors posts Resources on Addgene.org Fluorescent Biosensors...where should you begin? Have no fear, Addgene is here to help! General Design Figure 1: Two proteins...the FP back together (Figure 1). This method is generally known as Bimolecular Fluorescence Complementation...proteins in Caenorhabditis elegans. Goudeau et al. Genetics. 2021 Cynthia Kenyon mRuby4 mRuby4(1-10), pairs... -
Hot Plasmids Podcast Episode 2: New RFPs, AAVs, & More
TypeBlog PostPublishedSept. 8, 2017, 7:52 p.m....Toolbox for quickly generating zebrafish transgenic lines to study neurodegenerative diseases. Listen to...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast. Additional...Additional Resources on the Addgene Blog Listen to Our Podcast Interview with Eye Researcher Connie Cepko...Science Videos from Tom McFadden Resources at Addgene.org Sign up to Receive Our Newsletter Check Out ... -
CRISPR 101 eBook...en español
TypeBlog PostPublishedJan. 18, 2024, 2:15 p.m....disponible en español! Esta traducción fue regalada a Addgene por Daniel Mejías Sánchez, ¡y no podríamos estar...emocionados! Después de recibir la traducción, Addgene contrató a una traductora científica profesional...Descargar CRISPR 101: Una herramienta de edición Addgene tiene una gran audiencia internacional, incluido...available in Spanish! This translation was gifted to Addgene by Daniel Mejías Sánchez, and we could not be more...more excited! After receiving the translation, Addgene contracted with a professional scientific translator...Download CRISPR 101: Una herramienta de edición Addgene has a large international audience, including working... -
Enabling high school research at the Journal of Emerging Investigators
TypeBlog PostPublishedSept. 5, 2018, 12:01 p.m....Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast. Additional... In this episode of the Addgene Podcast, we introduce you to the Journal of Emerging Investigators, an...recently submitted to the journal with funding from Addgene. Listen to the interview with the Journal of Emerging...Additional Resources from the Addgene Blog Find education resources Use our Plasmids 101 eBook to teach ...genome enegineering technology Resources at Addgene.org Find basic molecular biology protocols Check... -
Selecting Your Plasmid Purification Kit
TypeBlog PostPublishedMay 27, 2025, 1:15 p.m....kit to use? Obtaining higher quantities of DNA generally means starting with higher volumes of bacterial...reduce your lab’s environmental impact, check out Addgene’s blog post on bringing sustainable practices for...Monarch®, QIAGEN’s QIAwave, and Milllipore Sigma’s GenElute™ kits were all specifically designed with environmental...Series NEB Monarch QIAGEN QIAwave Millipore Sigma GenElute Reduced waste from kit materials Reduced plastic...relatively little work after the initial setup. (Addgene actually uses some of these — if you’re curious... (These are mostly miniprep kits! Larger kits generally take longer.) Make sure to take a look at the...Nyla Naim. Resources Additional resources on the Addgene blog Plasmid Preps: Different Purity, Different...