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... streptavidin, both of which can bind up to four biotin molecules and can themselves be linked to a labeling... sodium azide, which can interfere with conjugation reactions. If needed, you can often remove the incompatible...spectrum, so they are great for multiplexing and can be used in a variety of assay types. Be sure to check...antibody conjugates in an earlier post, where you can find some additional tips for working with these ... which is light produced by a chemical reaction, can be detected using specialized imaging instruments...mass cytometry (Bendall, et al., 2012). And, you can also conjugate antibodies directly to beads for purification... linked to your antibody. This chemical coupling can be achieved through several different mechanisms....

...for clear information on costs. Can you suggest some coaches? Yes I can. Please note that Addgene does ... many scientists.  Sometimes it can be helpful having a coach that can offer extra support for issues ...Professional Career Coaching can be an excellent tactic for scientists making a career shift or who wish...wish to improve their current job situation. What can a coach do that is different from an adviser, boss...accessible. Sometimes only a few hours of coaching time can make an enormous difference so don’t assume that ...offices, postdoc offices or professional organizations can provide referrals. There are bad coaches out there... a good one that is a style match for your needs can take some research.  It helps to spend some time ...

...that can be done.  Note that each type of transfer requires different equipment. Wet transfers can be done... and can help amplify a weak signal. However, they have high cross-reactivity, vary significantly from...light-insensitive. They can detect femtogram levels of protein. These enzymes cannot be used for multiplexing...? What membrane? What should you block with? It can be so overwhelming that you might just stick with...monoclonal Both polyclonal and monoclonal antibodies can work well for a western blot. Polyclonal antibodies...batch-to-batch variation. Confirmation of sequence can be done from relatively cheap plasmid sequencing....of different rAbs that target the same protein — can be used to reproduce the benefits of polyclonal antibodies...

...final eluate. Bead-based kits can be more flexible in this situation as you can simply add more beads to your...Some applications require very little DNA and you can get away with slightly lower quality preps, while...Cloning Grade DNA (high quality/low quantity) – You can usually complete a cloning experiment with a relatively... on the experiment), but issues with DNA quality can inhibit important reactions like PCR or restriction... on the experiment, mammalian cell transfections can require quite large amounts of DNA (easily in the...DNA quality? There are a few different issues that can arise during the plasmid purification process. One...possibility of damaging the plasmid DNA itself, which can happen if you leave the sample in a lysis buffer ...

...procedure can be used to incorporate each element into the final construct. This strategy can be used to... each other, a third module cannot be placed between them, but rather, can only be added to the 5’ or ...synthetic biology for example, high-throughput cloning can be used to combine the functions of different genetic...reporter constructs. These fluorescent reporters can be used to detect structure and protein localization...sequences, and more. By combining these components, one can generate constructs to visualize cell cycle dynamics...directional, meaning that (in the previous example) one can control whether the third module is added to the ...References Golden Gate Type II restriction enzymes Can assemble up to 10 modules in a single step. Through...

...drugs, the diseases they can treat, and diagnostic advancements. Consultants can help these teams develop...of time can sometimes become cauldrons on the verge of boiling. On other occasions, clients can be excessively...are just examples, and healthcare consulting firms can be involved in many similar activities depending ...shifting goal posts” as a part of a case, consulting can sometimes be challenging. The fast paced nature of...working hours and the unpredictability of schedules can take on employee morale and productivity, and many... any highly collaborative work place, challenges can arise due to conflicting work styles, team situations...abruptly change needs and expectations. Such situations can get stressful and to help manage that, most consulting...

...the line, and can include assays for authentication and contamination) and cell lines can become cross ... The exact number of passages can vary between cells lines, as this can be dependent on the mutation rate...approach, the many mistakes of the past can be prevented and we can progress faster to desired clinical endpoints...retracted and there is a loss of reputation that can impede collaborations, especially with industry. ...test known as Short Tandem Repeat (STR) profiling can be used to ID the cell line. The same test is so ...forensic technique (aka DNA fingerprinting) that can place suspects at the scene of a crime (every human...human in the world has a unique STR profile) and can also be used as a paternity test. The test is accurate...

...into the brain can usually be achieved by a directed brain injection, but some serotypes can cross the blood...-associated viral vectors (AAV), neuroscientists can combine classical genetic tools with the manipulation...defined cell types. Specific transgene expression can be also achieved in nontransgenic animals by using... viral strategies Combining different approaches can increase the specificity of cell targeting even further...further. For example, injecting AAVs at one location can result in expression of an optogenetic payload in...the brain. A more locally defined set of neurons can then be targeted with optical stimuli. The restricted...targeted cell population.  Combinatorial strategies can also be used for retrograde tracing: visualizing ...

...what information is necessary and what can be left out, which can be a big challenge!  The resolution Throughout...glimpse into the world of science communication and can use what you've learned to share your science with...over again until you don’t.” As every researcher can attest to, science is nothing if not solving your...navigating conflict. In literary terms, conflict can be defined as (1) the struggle between two opposing... forward - and this drive is so powerful that it can entice an audience to come along on a journey even...or even by introducing a person that the audience can relate to. It doesn’t need to be anything fancy; ... provide enough information so that the audience can understand the conflict. A science-based conflict...

...….I can sense it….sound like a plot to an biology scifi movie? Or maybe it’s a biosensor?! You can do ...molecules What type of small molecules can you monitor with biosensors? You can keep tabs on my personal favorite... ‘sense’ biological compounds and reactions; you can quantify them with biosensors! In this blog we will...research are very sophisticated, but at their core they can all be boiled down pretty simply – they are systems...So now that we know what makes up a biosensor, we can discuss what flavors of sensors exist and are ready-to-use...physics, in this blog we will focus on those which can be applied in cell culture and animal models primarily...magnesium, potassium, and zinc. These ion sensors can be targeted to different cellular locations or tissues...