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... of a two-part interview with Vini Mani and Amy Gilson from Science in the News at Harvard University....your science, we sat down with Vini Mani and Amy Gilson from Science in the News, a graduate student organization...Podcast Many thanks to our guests, Vini Mani and Amy Gilson. Vini Mani is a graduate student at Harvard University...News, and member of the SITN podcast team.     Amy Gilson recently received her PhD from Harvard University...SITN podcast team. You can follow Amy on twitter @aigilson.   Special thanks to our sound engineer/producer...McFadden Learn about SITN's work at DayCon 2015 5 Reasons to Use Reddit for Science Communication ...

...changes in specificity than AAV-PHP.eB. For this reason, the Gradinaru lab anticipates that high-dose AAV-PHP.AX...happy transducing! This post was written by Rachel Leeson, with significant contributions from Cynthia Arokiaraj...Altermatt, M., Dobreva, T., Chen, S., Wang, A., Thomson, M., & Gradinaru, V. (2021). Deep Parallel Characterization...s41565-023-01419-x Deverman, B. E., Pravdo, P. L., Simpson, B. P., Kumar, S. R., Chan, K. Y., Banerjee, A....., Wang, Q., Katz, N., Buza, E. L., Bell, P., & Wilson, J. M. (2018). The Neurotropic Properties of AAV-PHP.B.... J., Bell, P., Raymond, A., Stanford, W. L., & Wilson, J. M. (2019). The GPI-Linked Protein LY6A Drives...journal.pbio.3002112 Jang, M. J., Coughlin, G. M., Jackson, C. R., Chen, X., Chuapoco, M. R., Vendemiatti,...

...them for use in your lab. Structural Comparison Functional Comparison Advantages of Nanobodies How to Use...for a comparison of nanobody, HCab, and traditional IgG antibody structures. Functional comparison Strong...Nanobody Toolbox Find Nanobody Plasmids Structural comparison As the name suggests, nanobodies are smaller ...Large: Advantages of Antibodies One of the main reasons antibodies are great indirect immunodetection tools... antibody incubation. See figure 4A below for comparison of this one-step staining with nanobodies vs ...

... is adapted from the 1979 Patterson Protocol. When published, the Patterson Protocol was shown to purify...DNA gel extraction method described in Kim and Morrison is nearly identical to that of commercially available...Purify DNA using glass filters. Image: Kim and Morrison, 2009. This method provides DNA yield and quality... kits for different purposes, with the Kim and Morrison method, you can just adjust the number of glass... protocol video library References Kim, Y., & Morrison, S.L. (2009). A Rapid and Economic In-House DNA...://doi.org/10.1038/s41598-018-30316-w  Zou, Y., Mason, M.G., Wang, Y., Wee, E.J., Turni, C., Blackall,...

...out their own novel experiments in semester two (Knutson et al., 2010a,b).      Fig. 1: Undergraduates...capabilities and laboratory and critical thinking skills (Knutson et al., 2010a,b). Classroom-based research projects...before a CURE trainee goes on to win a Nobel!   Jason Pellettieri is Professor and Chair of the Biology...Einstein Discovers America. New York./  Callahan, K., Peterson, C., et al. 2022. External collaboration results...planarians. Developmental Biology. 457:119-127. Knutson, K., Smith, J., Wallert, M., Provost, J. 2010a....and Molecular Biology Education. 38(5):317-323. Knutson, K., Smith, J., Nichols, P., Wallert, M., Provost...Education. 38(5):324-329. Kowalski, J., Hoops, G., Johnson, J. 2016. Implementation of a collaborative series...

...troubleshooting as advised by the technical support person. This delayed the progress of the project. After...familiarize yourself with technical support. Even having personal experience with technical support through the ...general positive attitude, and an open-minded personality. These traits will help you deal with any incoming...and there are also differences in customers’ personalities and their expectations. If you are willing to...is essential for success. For possibly this same reason, it can be one of the more constant and safe corners...simple way even if you don’t exactly know why. Personal and professional development in technical support...support gives you the opportunity to develop interpersonal and communication skills. Problem solving and...

...therapeutic applications. piggyBac (PB) transposon: The PB transposon is a mobile genetic element that transposes...heterogeneous iPSC cell lines, which can complicate comparisons made between lines. Incomplete silencing of transgenes...heterogeneity between clones. This can complicate comparisons made between samples, i.e. transgene expression... into cells as a donor plasmid containing the transposon, along with a helper plasmid that expresses the... is that genomic alterations can occur at the transposon insertion site, so sequence-verification is required... pluripotent mouse stem cells by the piggyBac transposon." Nature Methods 6, no. 5 (2009): 363-69. PubMed...Central PMCID: 2677165. 3. Jia, Fangjun, Kitchener D. Wilson, Ning Sun, Deepak M. Gupta, Mei Huang, Zongjin ...

...multiplex (Thellwell, 2000). See Table 1 for a comparison of features of several commonly used chemistries...sixfold variation in expression levels reported (Biassoni, 2014), so you’ll want to ensure that your reference...genes - and it is well worth your time to do one (Biassoni, 2014). At the bench As a highly sensitive technique... See Table 2, (adapted from Wong, 2018) for a comparison of different analysis methods.   Method ...defined log-linear phase   Table 2: A comparison of different qPCR analysis methods. Table adapted... master. References and Resources  References Biassoni R; Raso A. (eds.), Quantitative Real-Time PCR:...doi.org/10.1016/j.bdq.2017.11.001.Bustin SA; Benes V; Garson JA; Hellemans J; Huggett J; Kubista M; Mueller ...

... of a two-part interview with Vini Mani and Amy Gilson from Science in the News (SITN) at Harvard University...half of our conversation with Vini Mani and Amy Gilson from SITN, we discuss some of the many things you.... Many thanks to our guests, Vini Mani and Amy Gilson. Vini Mani is a graduate student at Harvard University...You can follow Vini on twitter @vinidhra.     Amy Gilson recently received her PhD from Harvard University...SITN podcast team. You can follow Amy on twitter @aigilson.   Special thanks to our sound engineer/producer...Science Careers 10 Steps to a Perfect Science Talk 5 Reasons to Use Reddit for Science Communication ...

...arise as a result of existing professional (and personal) relationships. However, most job seekers will...pile, it may never even be reviewed regardless of personal connections to the hiring group.  Read here for... Concern” as you will not likely know the exact person monitoring submissions (usually a Human Resources...mutual connection. If the organization is small, any person at the company might be able to help you. If the...LinkedIn is one great way to find an appropriate liaison, but you can use alumni databases, friends, and...make a difference. Having your resume forwarded personally gives you a much better chance at an interview...