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Showing: 61 - 70 of 630 results

  1. Career Insights: Technical Support Specialist

    Type
    Blog Post
    Published
    Nov. 9, 2017, 2 p.m.
    ...troubleshooting as advised by the technical support person. This delayed the progress of the project. After...familiarize yourself with technical support. Even having personal experience with technical support through the ...general positive attitude, and an open-minded personality. These traits will help you deal with any incoming...and there are also differences in customers’ personalities and their expectations. If you are willing to...is essential for success. For possibly this same reason, it can be one of the more constant and safe corners...simple way even if you don’t exactly know why. Personal and professional development in technical support...support gives you the opportunity to develop interpersonal and communication skills. Problem solving and...

  2. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ...multiplex (Thellwell, 2000). See Table 1 for a comparison of features of several commonly used chemistries...sixfold variation in expression levels reported (Biassoni, 2014), so you’ll want to ensure that your reference...genes - and it is well worth your time to do one (Biassoni, 2014). At the bench As a highly sensitive technique... See Table 2, (adapted from Wong, 2018) for a comparison of different analysis methods.   Method ...defined log-linear phase   Table 2: A comparison of different qPCR analysis methods. Table adapted... master. References and Resources  References Biassoni R; Raso A. (eds.), Quantitative Real-Time PCR:...doi.org/10.1016/j.bdq.2017.11.001.Bustin SA; Benes V; Garson JA; Hellemans J; Huggett J; Kubista M; Mueller ...

  3. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ...therapeutic applications. piggyBac (PB) transposon: The PB transposon is a mobile genetic element that transposes...heterogeneous iPSC cell lines, which can complicate comparisons made between lines. Incomplete silencing of transgenes...heterogeneity between clones. This can complicate comparisons made between samples, i.e. transgene expression... into cells as a donor plasmid containing the transposon, along with a helper plasmid that expresses the... is that genomic alterations can occur at the transposon insertion site, so sequence-verification is required... pluripotent mouse stem cells by the piggyBac transposon." Nature Methods 6, no. 5 (2009): 363-69. PubMed...Central PMCID: 2677165. 3. Jia, Fangjun, Kitchener D. Wilson, Ning Sun, Deepak M. Gupta, Mei Huang, Zongjin ...

  4. SciComm with the Experts at Science in the News Part 2

    Type
    Blog Post
    Published
    April 27, 2017, 2:30 p.m.
    ... of a two-part interview with Vini Mani and Amy Gilson from Science in the News (SITN) at Harvard University...half of our conversation with Vini Mani and Amy Gilson from SITN, we discuss some of the many things you.... Many thanks to our guests, Vini Mani and Amy Gilson. Vini Mani is a graduate student at Harvard University...You can follow Vini on twitter @vinidhra.     Amy Gilson recently received her PhD from Harvard University...SITN podcast team. You can follow Amy on twitter @aigilson.   Special thanks to our sound engineer/producer...Science Careers 10 Steps to a Perfect Science Talk 5 Reasons to Use Reddit for Science Communication ...

  5. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    Published
    July 9, 2015, 2:30 p.m.
    ...arise as a result of existing professional (and personal) relationships. However, most job seekers will...pile, it may never even be reviewed regardless of personal connections to the hiring group.  Read here for... Concern” as you will not likely know the exact person monitoring submissions (usually a Human Resources...mutual connection. If the organization is small, any person at the company might be able to help you. If the...LinkedIn is one great way to find an appropriate liaison, but you can use alumni databases, friends, and...make a difference. Having your resume forwarded personally gives you a much better chance at an interview...

  6. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    Published
    Oct. 2, 2018, 12:56 p.m.
    ...competence procedures Threats to personal standing – including undermining personal integrity, destructive innuendo... analytic scientists seem to toss data-driven reasoning out the window when making decisions. We think...think scientists make choices based on logic and reason, but often our decisions are based on emotions and... these labs in the first place. This is another reason to do your research and choose wisely. From the...members fight it out PI intervenes if there are interpersonal conflicts PI has no idea who is dating whom ...choose a grad school or postdoc lab. Having a comparison can be very helpful in uncovering strengths and...

  7. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ...University of Alberta studying with the Szymanski lab. Reasons to study how a phage recognizes its host Bacteriophages...molecules in molecular biology labs (for a review, see Simpson et al., 2015). Visit Addgene's MIcrobiology Pages...following is a description of a technique published by Simpson, Sacher, and Szymanski in January 2016 in Viruses... for physical shearing exist as well, such as sonication and needle shearing. Essentially, any method ...Callum J., Mohammadali Khan Mirzaei, and Anders S. Nilsson. "Adapting drug approval pathways for bacteriophage-based...27536293. PubMed Central PMCID: PMC4971087. 3. Simpson, David J., Jessica C. Sacher, and Christine M. ...Chemical Reviews (2016). PubMed PMID: 27319741. 3. Simpson, David J., Jessica C. Sacher, and Christine M. ...

  8. New Viral Vectors - Winter 2025

    Type
    Blog Post
    Published
    Jan. 28, 2025, noon
    ... James M. Wilson New serotype pENN.AAV.hSyn.Cre.WPRE.hGH AAV2 Recombinase James M. Wilson New serotype...James M. Wilson New serotype pENN.AAV.CamKII 0.4.Cre.SV40 AAVrg Recombinase James M. Wilson New serotype...FLEX-rev:: hM4D-2a-GFP AAV1 Chemogenetics Scott Sternson New viral prep pAAV-CaMKIIa-hM3D(Gq)-mCherry...resources!   This blog post was written by Rachel Leeson and Brian O'Neill.  More resources on the Addgene...

  9. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    Published
    Oct. 4, 2016, 2:30 p.m.
    ...PMC4246457. 4. Davidson CJ., et al. "Improving the limit of detection for Sanger sequencing: a comparison of methodologies...although this has been improved in some studies) (Davidson et al. 2012, Tsiatis et al. 2010). As the field... approaches can provide the researcher with a reasonable level of certainly that the effects they see ... PhD candidate in the laboratory of Prof Steve Jackson at the Gurdon Institute at the University of Cambridge...Vouillot, Léna, Aurore Thélie, and Nicolas Pollet. "Comparison of T7E1 and surveyor mismatch cleavage assays...PubMed Central. 5. Tsiatis, Athanasios C., et al. "Comparison of Sanger sequencing, pyrosequencing, and melting...

  10. Celebrate National Book Month with Addgene

    Type
    Blog Post
    Published
    Oct. 3, 2019, 12:47 p.m.
    ...Non-science favorites  The Mistborn series by Brandon Sanderson "The Mistborn series is the first 3 books of a...funny, bittersweet, and shocking at times."     - Jason Snair, Product Designer Sarum: The Novel of England...skeptical reader of historical fiction (I can personally attest to this!). Following history from the ...participated in a book club before, but I had a few reasons for launching this one. On a purely selfish level...contentious, discussions. I presented the first book, Nicholson Baker’s The Anthologist. The book ended up literally...Prejudice, and some more obscure treasures, like Tove Jansson’s The True Deceiver. There have been books that ...
Showing: 61 - 70 of 630 results