We narrowed to 644 results for: Uty

... their PhD and postdoctoral training. Addgene executive director Joanne Kamens has written a 5-part blog...pursue leadership opportunities at the local, institutional, regional, and national levels. These skills... But how does one gain such experience? Your institution may offer occasional workshops on leadership ...Harvard Medical School and then Boston University (institutions where I’ve worked as a postdoc). I soon realized...that many of the issues faced by postdocs and institutions are not local but are national issues. I applied...many changes to the  Board of Directors and the Executive Director changed twice. I had to learn how to ...to the Board of Directors and liaise with the Executive Director. As members changed, I had to learn how...

... positives [2, 4]. Solutions to the polymer problem There are some simple solutions available for researchers...GFP to study the protein's behavior in cells, in solution or something in between, you are using a tag with...ruin your day (and experiment) are listed below. Solutions to avoid these all too common issues follow. ...with all of these problems. The simplest and best solution is to use truly monomeric FPs. EGFP and other ...they are designated mEGFP, mVenus, etc. A word of caution is needed because FPs not derived from GFP, i.e...depend on protein concentrations. An imperfect solution is to restrict imaging analysis to cells expressing...

... sticking pipettes into solution bottles directly, rather decant the solutions into disposable sterile... up a routine to split cells Don’t be lazy about splitting cells, instead try to form a routine. Twice...that you also have the power to take the added precautions necessary to keep your cells from becoming contaminated...things more manageable, but it also keeps stock solutions from getting contaminated. Make sure cells don't...So if you, for example, split cells on a Monday diluting them 1:10, you should be able to split on the ...

...commonly used for elution, it can give more variable absorbance readings. A Tris-buffered solution tends to produce...readings to average out noisy measurements. Be cautious when interpreting purity ratios, as they can vary...vary significantly at low concentrations.  The elution buffer you use will also affect how accurate your...downstream applications and that you use the same solution for blanking your machine!  Finally, many NanoDrop...systems are no substitute for sound techniques, routine machine maintenance, and critical interpretation...pipette carefully? Remember to discard flow-through solutions and use clean tubes? How about spin times, were...

...which can reduce the potential image resolution of super-resolution fluorescence microscopy. Their size...about 2 nm, which can lead to higher resolution for super-resolution microscopy. Nanobodies can be labeled...animal sacrifice Poor tissue penetration Less resolution due to larger label displacement Require separate...penetration Can be labeled with multiple dyes Greater resolution due to lower label displacement 2° incubation... Nanobodies: site-specific labeling for super-resolution imaging, rapid epitope-mapping and native protein...

...vector solution, and generally these methods measure different characteristics of the solution. These ...can also be altered by freeze-thaw of the viral solution (Lock et al., 2010). Infectious titer: the ...quantified by electron microscopy of a viral vector solution. Since this technique is intensive and requires...requires an electron microscope, it is not routinely performed on all new viral vector preparations. Addgene...facilities report the physical titer of a viral solution (Figure 2). Because physical titers are used for...starting template is the purified viral vector solution (which contains viral capsid proteins) as opposed...

...Blainey, P.C. (2024). CROPseq-multi: a versatile solution for multiplexed perturbation and decoding in pooled...valuable tool for microglia research and broader therapeutic applications.  Find microglia-targeting AAV plasmids...https://doi.org/10.1101/2023.12.12.571321.  Revolutionizing Gene Therapy: The REVeRT Dual AAV Vector System...has developed REVeRT, for gene delivery using reconstitution via mRNA trans-splicing. By splitting a large...Illustration of split fluorophore assay to test reconstitution via mRNA trans-splicing. A cis-splicing vector... The authors used REVeRT to functionally reconstituting coding sequences for a range of applications...mechanisms of delivery and the potential for therapeutic success. Find REVeRT plasmids here! Riedmayr...

...Structural problems in academic research and potential solutions During the first couple of sessions of the symposium...his or her chosen career path,” but individual institutions can have many different names for the positions...From Panel Session 2: Defining the Postdoc: An Institutional Perspective. This lack of complete information...workforce, and we are beginning to identify potential solutions. Keynote speaker Paula Stephan did a fantastic... articulating many of these problems and some solutions. The structural problems seem to boil down to ...here). Importantly, the panel offered some great solutions to these problems and some that stuck out to me...those at the FOR symposium and will enact their solutions as soon as they become actionable. Additional...

...the complex interactions between cancer cells, therapeutic drugs, and the rest of the body than studies ...or cell culture. For example, Scott Lowe’s lab utilized mice to study how different mutations in leukemia...important human disease model with potential for therapeutic drug discovery as it is estimated that 75% of...they can also be used to easily screen multiple therapeutic compounds in one experiment. Zebrafish (Danio...to name a few. For example, Andrew Leifer’s lab utilized the worm to study calcium signaling in the entire...al. "Whole-brain calcium imaging with cellular resolution in freely behaving Caenorhabditis elegans." Proceedings...Drosophila melanogaster and the role of the fly in therapeutic drug discovery." Pharmacological reviews 63.2...

...cytosine-to-thymine substitutions in target sequences (Banno et al., 2018). These substitutions were limited ...the utility of cytidine and adenine base editors that enable C-to-T and A-to-G base substitutions respectively... is done with recombineering, a technique that utilizes phage recombination machinery to promote homologous...especially for larger modifications. What’s the solution to this inefficiency? Use CRISPR to make it a ...introduce point mutations in P. aeruginosa, Pseudomonas putida, Pseudomonas fluorescens, and Pseudomonas syringae...respectively. Substitutions seem most efficient when using a nickase version of Cas9, but were also successful...