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We narrowed to 331 results for: nans

Showing: 61 - 70 of 331 results
  1. Rabies and Neuronal Tracing

    Type
    Blog Post
    Published
    May 29, 2018, 1:51 p.m.
    ...neural connectivity (Nassi et al. 2015). The recombinant viruses typically used in neuroscience research...transmitted transsynaptically). This is because the recombinant forms of these viruses typically used in research...characteristics of a G-deficient rabies virus recombinant: an in vitro and in vivo study. J Gen Virol. ...Osakada F, Callaway EM. Design and generation of recombinant rabies virus vectors. Nat Protoc. 2013;8:1583...
  2. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    Published
    June 27, 2017, 1:03 p.m.
    ... determine whether two proteins are within 10 nanometers of each other, or measure the tension in the ...    What is this FRET you speak of? Förster Resonance Energy Transfer (FRET) was originally described...has led to the alternative acronym Fluorescence Resonance Energy Transfer. Unlike the typical excitation...Müller, Sara M., et al. "Quantification of Förster resonance energy transfer by monitoring sensitized emission...
  3. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    Published
    Aug. 9, 2022, 1:15 p.m.
    ...general availability of their recombinant antibodies. Since recombinant antibodies are a newer technology...chose monoclonal antibodies (mAbs) and their recombinant forms (rAbs). mAbs and rAbs have higher homogeneity...ValidationSelecting the Right AntibodyPlasmid-based Recombinant Antibodies    ...
  4. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type
    Blog Post
    Published
    June 25, 2020, 1:15 p.m.
    ...might not be the right choice. I spoke with our Financial Operations Specialist, Lucy Hammer, about why ...response to this question came from Lucy Hammer, our Financial Operations Specialist. She said, “I’ve found the...came to Addgene with a goal to learn nonprofit finance, and while I've done that, my big takeaway has ...conversation master knows that although her focus is finance, it is just as important to bring love and fun ...
  5. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...biosensor consists of a YFP-tagged G protein and a NanoLuc® (Nluc) Luciferase-fused detector that specifically...://doi.org/10.1016/j.cell.2024.01.028. New recombinant antibodies for neurodegeneration research   By...-MAPT [AT8] and Anti-APP [6E10-A5]. Both are recombinant mouse monoclonal antibodies targeting the human...Immunohistochemistry with Addgene's Anti-APP [6E10-A5] recombinant antibody in human postmortem brain samples. Results...
  6. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    Published
    July 16, 2024, 1:15 p.m.
    ...Expression in Cultured Cells Using Unpurified Recombinant Adeno-Associated Viral Vectors [Preprint]. Molecular... K., Parianos, M., Truong, V., Rostonics, E., Hernandez, A., Boye, S. L., Boye, S. E., Levites, Y., … ...Packaging of genomes can limit production of recombinant AAV-2. Gene Therapy, 6(7), Article 7. https:/... Snyder, R. O. (2010). Characterization of a recombinant adeno-associated virus type 2 Reference Standard...
  7. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...Tagging Plasmid-based Recombinant Antibody Resources on Addgene.org  Find recombinant anti-c-Myc [9E10] ..., 1984). The strategy they landed on was to recombinantly append their proteins of interest with unrelated...have moved to producing anti-Myc [9E10] as a recombinant antibody. Researchers first sequenced the variable...
  8. PhD Applications After COVID

    Type
    Blog Post
    Published
    Jan. 5, 2023, 2:15 p.m.
    ... program is the right fit for you without the financial and logistical stress that accompanies traveling...faculty, students, and each other. Before COVID-19, financial and logistical obstacles could make touring potential...COVID-19? How are students funded? Are there any financial aspects that are confusing? During a traditional...interviews for a variety of reasons (logistical, financial, etc…) now have options to fully engage in the...
  9. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ...repairing DNA double strand breaks – (HR). HR is the dominant homology-directed repair (HDR) pathway in mammalian...your cells. Enrich S-phase cells HR operates predominantly in S and G2 phase of the cell cycle, when a ...20): 1-9 (2020). 10.1038/s41589-019-0432-1 Mehdi Banan. Recent advances in CRISPR/Cas9-mediated knock-ins...2014) 10.7554/eLife.04766 Moreno-Mateos, M. A., Fernandez, J. P., Rouet, R., Vejnar, C. E., et al. CRISPR-Cpf1...
  10. Technical Design of a Western Blot

    Type
    Blog Post
    Published
    Aug. 22, 2024, 1:15 p.m.
    ...-lot. The highest reproducibility comes from recombinant antibodies (rAbs), which are produced from plasmids...plasmid sequencing. While rAbs are monoclonal, recombinant multiclonal antibodies — mixtures of different...non-specific binding; lot-to-lot variability Some recombinant antibodies Not always available; not always... Monoclonal antibodies Increased cost Recombinant antibodies Not always available; cost varies...non-specific binding; lot-to-lot variability Recombinant multiclonal antibodies Not always available...
Showing: 61 - 70 of 331 results