We narrowed to 432 results for: sequence

...expected sequence. Here at Addgene, we use NGS-based quality control to confirm the sequence of all the...cleaving sequences which are similar, but not identical, to their defined recognition sequence. Most enzyme...successfully even if you don't have the full plasmid sequence. Once you have purified plasmid DNA, this method...NCBI for YGOI to find the particular reference sequence if necessary. The example plasmid on the right... dna sequencing results...

...CRISPR enzymes require a short PAM sequence adjacent to the targeted sequence at the locus of interest. SpCas9...engineered alternative Cas9s binding distinct PAM sequences. Now, Hu et al., working in David Liu’s lab, have...PAM-interacting domain. However, these alternative PAM sequences are longer and often more complex than NGG, resulting... for and interest in enzymes with shorter PAM sequences and/or broader PAM flexibility. Creating xCas9...plasmid library contained all 64 possible NNN PAM sequences adjacent to a protospacer. If ω-dCas9 could recognize...permitting phage propagation and keeping that dCas9 sequence in the pool. According to this logic, ω-dCas9 ...off-target effects across 5 gRNAs with NGG PAM sequences were actually lower for xCas9 3.6/3.7 than for...

...adding a nuclear localization sequence (SV40 Large T nuclear localization sequence) and expressing it from ... of the DNA sequence of interest can lead to poor expression of genes within that sequence and should ...between target sites of the same sequence in FLEx vectors, the DNA sequence that should be inverted is flanked...differ in their sequence. Recombination between the two target sites with identical sequence leads to inversion...when it was discovered that it is not the exact sequence of the asymmetric target site core but its 8 bp...inversion of the flanked DNA sequence and the interior target sites. In a second step, the two compatible...

...recombinant channelrhodopsin sequences. The model first removed sequences from the library predicted to...reducing the library to 1,161 sequences. Thirty of these remaining sequences were selected for characterization...to have improved photocurrent properties: The sequence and structures that determine membrane expression...model was trained to predict if a channelrhodopsin sequence would result in a functional channelrhodopsin,...library of ~120,000 predicted channelrhodopsin sequences would have photocurrent properties best suited...for less-invasive optogenetics experiments. The sequences in this library were designed using structural...

..., a SSR will invert the DNA sequence between the sites. If a DNA sequence is floxed in the same orientation...the BFP coding sequence in the sense orientation, followed by the mCherry coding sequence in the antisense...reporter DNA sequence (such as GFP) in the sense orientation, followed by coding sequence of the gene ...loxP sites, while FLP binds FRT sites. When a DNA sequence is flanked by target sites (floxed) in opposing...loxP and lox511 sites after the inverted GeneR sequence in the opposing orientation, you have created ...

...anticodon,  a three nucleotide sequence complementary to the mRNA sequence. The 3’ end of  the tRNA binds...the amino acid that corresponds to the anticodon sequence. That means there are 61 possible tRNAs. Cells...and thus protein expression without altering the sequence of the protein. As DNA synthesis has become quite...scientists to re-synthesize DNA with the most optimized sequence. Unfortunately, it is not always easy to identify... certain systems, synonymous changes to a gene sequence can cause unexpected detrimental results to the...could remove information encoded in the primary sequence of a particular gene which could affect translation...available online to both determine and synthesize DNA sequences for optimal expression in your system of choice...

...They found that DNA sequences targeted by the gRNAs were the top enriched sequences in the experiment and...supporting roles. Enhancers are cis regulatory DNA sequences that, when bound by transcription factors, can...looping with the photo “developed” by analyzing DNA sequencing data. While “pictures” generated with current...chimeric DNA fragments by PCR or next-generation sequencing. Figure 1: Overview of the key steps of... DNA fragments can be detected by PCR or deep sequencing. A drawback of the original CAPTURE method...gRNA expression. Additionally, CAPTURE 3C-based sequencing requires a large number of cells (~5x107). Together...BioTAP-tag is a 69 aa long biotinylation targeting sequence that is recognized by biotin ligases normally ...

...scaffold sequence into the genome, ensure it is not homologous to the target genomic sequence (Chen et...experimental design, especially the design of the RNA sequences you’ll use in prime editing. We thought we’d collect...both the genomic location to be edited and the sequence of the edit, so its design changes for every new... prime editing application. And optimizing its sequence is important, as prime editing efficiency is affected...content are most likely to be successful, although sequences with G/C content outside this range can still ... more important to test different lengths and sequences, as unintended secondary structures in your pegRNA...Created with BioRender.com. What to edit The sequence you choose to install can influence the success...

...desired sequences. TALENs are much more reliable. If you design them for a particular sequence, there ...can be programmed to bind to essentially any DNA sequence desired. This technology allows you to create ...do you use this kit to target a particular DNA sequence? Musunuru: The kit includes 832 plasmids containing...DNA-binding domain to recognize any desired 15 base pair sequence in the genome in rapid fashion. The two remaining...there is a much better chance they will bind that sequence. That’s the revolutionary aspect: TALENs take a...: The power here is that you could potentially sequence a large number of individuals and find several...

...cleave a targeted sequence of ssDNA. Unlike Cas9, Cas14 does not require a PAM sequence. In addition to ... types of nucleic acids and required a certain sequence specificity in the middle of the gRNA to be activated...mutations. By providing a gRNA specific of a viral sequences, for instance, the enzymes are activated and can...Fluorescent signal indicates the presence of this viral sequence in the assay. By adding Cas14 to the mix, you ...