We narrowed to 279 results for: Plat;

...requires the much less efficient double stranded templates, rather than the more efficient single-stranded...cellular protein. They used CRISPR with a ssODN template to introduce the small wrmScarlet11 which is less...

...typically used (e.g., use 5 mL medium on a 10 cm plate). By reducing the culture volume into which the ...vector using the harvested viral solution as a PCR template. Regardless of the type of virus you’re working...

... have members looking at how to re-use it as a platform for monitoring homemade microfluidic chips using...using the fantastic optics and fluidics platform it contains. Biotech equipment becomes obsolete amazingly...

...solution. PEG takes several hours on a heated stir plate to dissolve and requires monitoring so that the ...plasmid, not that I would know about this… A no template control (NTC) should always be included. While...

... vector and your insert, DpnI digest away the templates, and transform the PCR products into E. coli. ...will require a PCR reaction or two to generate templates for the cloning process. Time spent actually doing...

... on Friday. It’s best to split cells before the plates are more than 80-85% confluent because cells like...Benjamin Franklin)! It’s good practice to label your plates and/or flasks with your name, date, the cell line...

...signaling in primary cells. The authors used this platform to assay dozens of GPCRs across many cell types...context-dependent GPCR activity with a universal biosensor platform. Cell, 187(6), 1527–1546.e25. doi: https://doi.org...

...Cutting both strands ensures that there isn’t a template strand left behind that the cell can use to repair...combination of chewing up one stand and cutting the template strand causes large deletions of up to hundreds...

...universities!) This guide tells you how to use online platforms, such as Zoom, to determine if a program is the... yours does and then use that time! Different platforms have different quirks, so remember to practice...

...experiment worked just by looking at the cells on the plate. For example, if your plasmid contains a gene encoding... Chromoprotein expression from bacteria on agar plate. Image: Liljeruhm et al., 2018. For each chromoprotein...

...may lead to loss of the plasmid, and using older plates could increase plasmid loss or mutation. Let it...smaller cultures, especially those in deep-well plates, require a faster shaking speed for proper aeration...

...publication. Addgene makes this easy by providing a template citation for you to use in your Materials and ... Use the Addgene catalog number to easily find template citations for Addgene plasmids you have used in...

... up after trying out the new protocols.io beta platform for sharing, annotating, and storing life science...molecular cloning protocols, including making LB agar plates, restriction digest of plasmid DNA, and bacterial...

...important to understand that these platforms, or pieces of a platform, that are now available exist within...

...Addgene launched our AAV Data Hub as a community platform for the sharing of that exact data. Here, requestors...develop new systems, new partnerships, and new platforms, from our early engagement with UPenn to our launch...

...modifiers available. This system utilizes an RNA template containing the desired edit adjoined to the sgRNA...affect gene expression? dCas9 can be used as a platform to deliver various cargo to a specific DNA locus...

...amplification. Transformed E. coli are allowed to grow on plates or in liquid culture to amplify the library, followed...Joung J, Konermann S, Gootenberg JS, Abudayyeh OO, Platt RJ, Brigham MD, Sanjana NE, Zhang F. Genome-scale...

...is a factor in AAV titration since the starting template is the purified viral vector solution (which contains...be mitigated by using purified viral DNA as the template instead of the intact AAV particle. Finally, ...

...ummmm, what is it? Reddit is a volunteer-organized platform where users can participate in forum discussions...to see the questions first makes Reddit a great platform for those who aren’t so great at coming up with...

...concentrations of lactate. The microfluidic screening platform developed here is also a promising approach for...other targets. Figure 6: A) Schematic of BeadScan platform for screening candidate biosensor variants for...