We narrowed to 511 results for: ATO

1. Genome Engineering, CRISPR, and Research in Singapore: Interview with Wei Leong Chew

   Type

   Blog Post

   Published

   Feb. 6, 2018, 2 p.m.

   ... Singapore. Chapter 4: Life as a new primary investigator. Coming up with new project ideas, applying ... Chew! Wei Leong Chew is a Junior Principal Investigator at the Genome Institute of Singapore. His lab...

2. Celebrating the 2024 Nobel Prize in Chemistry

   Type

   Blog Post

   Published

   Oct. 17, 2024, 1:15 p.m.

   ...structure is strikingly similar to the design model at atomic resolution” (Kuhlman and Dantas et al., 2003). ...). Design of a Novel Globular Protein Fold with Atomic-Level Accuracy. Science, 302(5649), 1364–1368. ...

3. New Viral Vectors - Spring 2025

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   Blog Post

   Published

   April 29, 2025, 1:30 p.m.

   ... retrograde serotype, and a dTomato under the control of the E6 regulatory element, which targets it to...

4. How Exercise Purges Stress and Keeps Depression at Bay

   Type

   Blog Post

   Published

   Nov. 25, 2014, 4:02 p.m.

   ...Cell demonstrate how PGC-1a1, a transcriptional coactivator induced in skeletal muscle by exercise and endurance...altogether. Their muscles showed an increase in inflammatory cytokines as a result of those mild stressors...

5. Q&A With Julie Cicalese, the Newest Member of the Addgene Board of Directors

   Type

   Blog Post

   Published

   March 17, 2021, 2:40 p.m.

   ...included the Belmont Human Rights Commission and mediator for the Massachusetts small claims court summary...Oncology and the Chicago Marathon to benefit Neurofibromatosis, New England. Additional resources on the...

6. The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years

   Type

   Blog Post

   Published

   Jan. 8, 2014, 3:10 p.m.

   ..., from zinc finger nucleases to transcription activator-like effector nucleases (TALENs). Our most popular... GCaMP6 series of genetically encoded calcium indicators were the hot technology of the year in our collection...

7. Let There Be LITE Plasmids

   Type

   Blog Post

   Published

   Nov. 21, 2013, 3:56 p.m.

   ...customizable DNA-binding domains based on transcription-activator-like effectors (TALEs) as anchors for genome engineering...and modular,” Konermann said. “Transcriptional activators are easy to expand or combine with different ...

8. Editor's Choice, August 2016

   Type

   Blog Post

   Published

   Sept. 2, 2016, 1:32 p.m.

   ... bit of a Twitter storm surrounding NgAgo, its creator, Han Chunyu of Hebei University of Science and ...Transductions Single Base Editing with CRISPR Cas9 Activators: A Practical Guide 10 Steps to a Perfect Science...

9. Modulate the Activity of 17 Signaling Pathways with One Kit!

   Type

   Blog Post

   Published

   March 22, 2016, 2:30 p.m.

   ...environments, for example. “We’ve been contacted by investigators all over the world who are interested to use...researchers have plans to expand the library to include activators for more cancer-related pathways and more nodes...

10. Inside Addgene's 10 Year Anniversary Gala

    Type

    Blog Post

    Published

    July 3, 2014, 3:48 p.m.

    ...hour, we feasted on sliders, hot dogs and a mashed potato bar (complete with all the fixings), as well as...about her grandfather’s humble beginnings as an operator of a printing press. Just as her grandfather used...

11. SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture

    Type

    Blog Post

    Published

    Aug. 13, 2014, 3:49 p.m.

    ...this purpose? What led you to it? Howarth: Our collaborator Uli Schwarz-Linek at St. Andrews discovered ... are useful for mechanical study of proteins by atomic force microscopy (AFM). Maybe polyantibodies could...

12. 9 Tips to Achieve Success in Academia

    Type

    Blog Post

    Published

    April 1, 2014, 5:33 p.m.

    ...for students, postdocs, and young Principal Investigators (PIs). We've interviewed Addgene depositors ...different ways to succeed – as loner or gregarious;  dictator or laissez-faire; theory or practice; business-like...

13. New Viral Vectors - Fall 2024

    Type

    Blog Post

    Published

    Oct. 3, 2024, 1:15 p.m.

    ...Esteban Engel New serotype pAAV-hSyn-Cre-P2A-dTomato AAV PHP.eB Recombinases Rylan Larsen New serotype...multiple serotypes pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato] AAV1 Optogenetics Edward Boyden New serotype ...

14. Build Your CRISPR Vocabulary

    Type

    Blog Post

    Published

    July 2, 2024, 1:15 p.m.

    ...used to target complementary DNA sequences. In laboratory settings, an sgRNA includes the crRNA and tracrRNA...itself, simplifying the CRISPR-Cas9 system for laboratory applications. At Addgene, we sometimes refer ...

15. Antibodies 101: Affinity Tags

    Type

    Blog Post

    Published

    Sept. 19, 2023, 1 p.m.

    ...applications. Also, purifying GST by affinity chromatography requires proper folding of the tag, which prevents...to be purified by single step metal affinity chromatography. Purification of His fusion proteins from E...

16. Cellosaurus: A Cell Line Information Database

    Type

    Blog Post

    Published

    Feb. 21, 2023, 2:15 p.m.

    ...line is known to be genetically manipulated, its anatomical origin, if cell line has ever been the target...informative. Spoiler alert: HeLa is the most common contaminator line.  For those wanting to access Cellosaurus...

17. The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

    Type

    Blog Post

    Published

    Jan. 21, 2020, 2:30 p.m.

    ...metadata for each screen is captured by BioGRID curators, including the cell line/tissue type, enzyme system...CRISPR database are encouraged to contact BioGRID curators at [email protected] to submit their published...

18. Oh, The Places You Can Go: Careers in Science Communication - Product Development

    Type

    Blog Post

    Published

    Oct. 18, 2017, 1 p.m.

    ...through Caitlin’s blood. “I come from a family of educators, mostly elementary school, middle school, and ...school, I actually served as the student course coordinator. With the guidance of one of the faculty members...

19. Oh, The Places You Can Go: Careers in Science Communication - Science Writing

    Type

    Blog Post

    Published

    Oct. 3, 2017, 12:11 p.m.

    ...For this series, I’ll interview three science communicators who work in the biotech, education, or nonprofit...job. “I am really more of a multimedia science communicator than a science writer these days. I do some ...

20. Sleeping Beauty Awakens for Genome Engineering

    Type

    Blog Post

    Published

    June 30, 2015, 2 p.m.

    ...of DNA that can move around in a genome. In a laboratory setting, transposons can be used to both introduce...use of viruses is also important for social and regulatory reasons. 1.) The transposon consists of a mirrored...

21. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

    Type

    Blog Post

    Published

    Nov. 12, 2019, 2 p.m.

    ... able to eliminate the need for axenic (pure) laboratory cultures to study these microbes. From 16S rRNA...between the battery bank and the vehicle battery/alternator to make sure the vehicle battery didn't run down...

22. Arabidopsis in Education: How the Arabidopsis Biological Resource Center Brings Plant Science to Life

    Type

    Blog Post

    Published

    April 12, 2018, 1:02 p.m.

    ... more than 100,000 samples to researchers and educators in 60 countries each year. Introducing Arabidopsis...Science and Industry (COSI) and the Franklin Park Conservatory. Do you use Arabidopsis in your research? Are...

23. Single-cell tracking of lineage and identity with CellTag

    Type

    Blog Post

    Published

    May 9, 2019, 1:15 p.m.

    ...Washington University in St. Louis provides a combinatorial indexing strategy that simultaneously tracks...2019). Key takeaways of CellTag CellTag is a combinatory cell-indexing tool that combines scRNA-seq and...

24. 10 Great Guest Posts We're Thankful For

    Type

    Blog Post

    Published

    Nov. 18, 2016, 3 p.m.

    ...them. CRISPR Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening CombiGEM is a technology that...Albert Cheng and Mark Wanner from the Jackson Laboratory for Genomic Medicine described Casilio, a system...

25. Using Addgene's New Viral Service for Your Research

    Type

    Blog Post

    Published

    Nov. 17, 2016, 3:30 p.m.

    ...delivery of Cas9 endonuclease, Cas9 nickase, Cas9 activators, Cas9 repressors, select gRNAs, and CRISPR pooled...expression of a gene under the control of the tet operator. AAV AAVs are the workhorses of both chemo- and...

26. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type

    Blog Post

    Published

    April 9, 2020, 1:15 p.m.

    ...following up on these leads as we speak, with collaborators in Paris and New York. We want YOU to join the...impact on virus replication. While we and our collaborators are starting these experiments, there's so much...

27. Summer SciComm Series: A PhD in Science Communication

    Type

    Blog Post

    Published

    Aug. 23, 2022, 1:15 p.m.

    ...For my undergraduate degree, I majored in human anatomy, but I also took classes in biochemistry, microbiology...the time I was ready to graduate with my BSc in Anatomy, the University of Otago had established the Centre...

28. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type

    Blog Post

    Published

    April 7, 2022, 1:15 p.m.

    ...SF-Venus-iGluSnFR). iGluSnFR3: Improved Glutamate Indicators for Synaptic Imaging However, the first two generations... 30377363 Aggarwal, Abhi, et al. . “Glutamate indicators with improved activation kinetics and localiation...

29. Celebrate National Book Month with Addgene

    Type

    Blog Post

    Published

    Oct. 3, 2019, 12:47 p.m.

    ...relevant science."     - Randall Carter, Contracts Administrator Inferior: How Science Got Women Wrong - and ... Tsang, Science Communications and Marketing Coordinator Non-science favorites  The Mistborn series by...

30. New and Upcoming Viral Vectors - June 2019

    Type

    Blog Post

    Published

    June 18, 2019, 1:15 p.m.

    ...37825  AAV1 pAAV-CAG-GFP 59462  AAV2 pAAV-CAG-tdTomato (codon diversified) 114472  AAV5, AAV8 pAAV-...ppAAV-CKIIa-stGtACR1-FusionRed New tetracycline transactivators Plasmid Serotype Name 99120  AAV1 pAAV-ihSyn1...