We narrowed to 798 results for: KIN

...Novartis Oncology.  Bhang did her PhD work at Johns Hopkins and came to Novartis for her post-doc in January...after treatment with EGFR inhibitor, erlotinib. Looking at barcode abundances among multiple replicate ...Pharmacology group at Novartis Oncology, Bhang has been working on using the ClonTracer library to monitor clonal...their own work. She says, “I spend a lot of time packing, shipping, and sharing protocols one by one,” and...is a “really great way of broadly sharing these kinds of reagents.” Here at Addgene, we’re excited to ... to use! Many thanks to Carrie and Novartis for taking the time to talk with us and for providing this...

...proteins had to be re-engineered for each gene, making wide-scale gene activation seem next to impossible...activation uses dCas9, a CRISPR protein variant lacking its endonuclease ability, to bind to genes without...there are multiple options to choose from when looking to activate genes across diverse cell lines.   ...activation compared to other CRISPR activators, making it a popular method for gene activation experiments...design (SunTag). This streamlines its delivery, making it a common choice for CRISPR activation. Newer... Larson MH, Gilbert LA, Doudna JA, Weissman JS, Arkin AP, Lim WA (2013) Repurposing CRISPR as an RNA-Guided...

...started working in the pharmaceuticals industry after earning my Master’s in 2016 and then working four ...prepare 1-2 unique questions for each interviewer by looking at their LinkedIn profiles. This can help spark...your 1-hour presentation to your audience Start by asking the hiring manager or recruiter what presentation...improving a process or procedure, solving a problem, or making a key decision. Even deciding to stop pursuing ...sharing your screen and won’t be able to see who’s asking a question. Maybe you ask for questions after completing...

...deposits per month!), manual transformations were taking a lot of time. Enter the Biomek FXP! We wanted ...improve our transformation protocol and, after looking at a few options, we decided on Beckman Coulter...protocol on the Biomek FxP is pretty simple. By clicking a few buttons you can quickly tell the Biomek ...pick up 8 tips and position the tips indepently making it easy to spread out your samples. This can be...scientists share plasmids across the globe, we’re working on ways to send plasmids as DNA as opposed to in...it suits our plasmid prepping needs perfectly. Working with Qiagen, we developed a protocol to run up ...

...the hopes and concerns of these communities. This kind of engagement and two-way dialogue is crucial if...advising writers and directors in Hollywood; and working directly with community organizations, libraries...leadership and communication experience. These included taking extra coursework and attending seminars in bioethics...writing science articles for a lay readership, and working with middle and high school students. One of these...organizations was pgEd. After obtaining my PhD and working in digital academic publishing for a year, I’ve...and in helping you decide whether you enjoy this kind of work. It is also beneficial to hone your non-...

... (and Addgene depositor) Craig Mello. Taking his groundbreaking work in RNAi one step further, his lab...the majority of attendees were scientists still working at the bench. It was a fantastic opportunity to...cell membrane. His recent work shows that they are making fairly remarkable progress on establishing a system...the drug resistantance genes in the bacteria thus making the bacteria sensitive to known antibotics. These... CRISPR resources Read Joanne Kamens' Post on Networking at Scientific Conferences  ...

... gRNA using restriction enzyme BsaI. If you’re working in Drosophila, a two-gRNA expressing plasmid is...outside of their recognition sequence, creating flanking overhangs. These overhangs can be customized to...vectors each contain Type IIS restriction sites flanking the promoter-gRNA construct, but with different...with the appropriate Type IIS enzyme, the unique flanking overhang sequences can link together to allow ...constructs with multiple promoter-gRNA cassettes, making them advantageous for small capacity vectors like... mammalian option described below, plasmids for making polycistronic gRNAs are also available from the... tyr gRNA, which causes hypopigmentation, thus marking cells that have undergone genomic modification....

...between skin and muscle 25–27 < 2–3 mL (~1 mL per site) Beginner Intradermal Anesthesia Between skin layers...Subcutaneous (SQ or SC) injections are given between the skin and the muscles. The most common injection sites... (ID) injections occur between the layers of the skin. While this could theoretically be performed anywhere...shaved first. Restraint: anesthesia Location: between skin layers Gauge: 26 Volume: < 0.05 ml Skill required...cringe or your experiment will allow you to avoid poking the mice, these “no needles” techniques may come...

...mentioned above basically involve taking the Myc1-9E10 variable regions and sticking them onto different scaffolds...tagging strategy, another group of researchers was working on isolating monoclonal antibodies to better probe...used in way more applications.   Fig. 1 Making a Protein Tag (Abridged) (1) Locate a promising...same antibody developed almost 40 years ago? Well…kinda. In some cases, antibody providers are distributing.... 1997; Schiweck, et al. 1997) with the goal of making the antibody more amenable to protein engineering...

...and feel the satisfaction of checking off each step as you painstakingly complete your experiment – one...Addgene Website: http://www.addgene.org/ If you’re working with plasmids and you decide to share them using...of useful molecular cloning protocols, including making LB agar plates, restriction digest of plasmid DNA.../ Labguru is a good place to go if your lab is looking to streamline your lab management and you want ...reagent storage and inventory, managing orders, tracking students and research projects, electronic lab...

...proficiency.  What kind of content are you sharing?  Sometimes your subject matter will drive the kinds of communications...others will avoid it like the plague. Instead of looking for The One Method to Rule Them All, consider the...embark on a project, do your best to understand what kind of resources - including your time - will be required...Podcasts Medium Easy for audience to engage while multitasking. Easy to host guests remotely. Easy access from...things to be physically interacted with. Public speaking is a common fear. Can be discouraging to build...

...in “crispr” and see what comes up. Before even clicking the ‘Search’ button a new content-aware suggestion...jump directly to the type of information you are looking for in our catalog or educational resources: plasmid... your lab. Instead of starting a new search or clicking through to a different part of the website, you...been deposited? In this example we’re going to be looking for recombinant monoclonal antibody plasmids from...finger protein involved in neurodegeneration in Parkinson’s disease. Knowing the primary investigator where...functionally (e.g. Base Editing) and by target organism. Clicking through to RNA Editing brings us to a page with...

...large-scale CRISPR screens in cancer cell lines, looking to uncover genetic dependencies of individual tumors...dependencies across ancestry groups (right).    Seeking an explanation  First, they looked for a biological...unexpected discovery, which didn’t make sense in any kind of biological framework, suggested to them that ...information they needed to begin correcting the issue. Working with James McFarland, Jeremie Kalfon, Javad Noorbakhsh...CRISPR guides mapped to a SNP would be ignored. Working with Lia Petronio and Katherine Huang from Broad...process does not stop there. Currently, they are working with Broad's Genetic Perturbation Platform to modify...

...employees spent this remaining time by elaborately pranking each other, dressing in costume, and holding competitions...the question, “What’s your favorite part about working at Addgene?” Most Addgenies reply with an enthusiastic...collaborators have completed their work thoroughly. It’s kind of a chicken and egg situation- do we work so hard...rose-colored-glasses-esque picture of how everyone is kind and collaborative and all we do is have fun all ... To keep the organizational side of things from taking too much of your time, scroll down for some tips...fall into that rut! We’ve done everything from kayaking, to field day, to relay triathlons, to 5Ks. Many...

....g. 5’-GGAG and 3’-TACT flanking promoter parts, 5’-AATG and 3’-GCTT flanking CDS parts, etc). These overhangs... Level 1 vector with a promoter and terminator flanking the CDS, and a Level 2 multigene vector with two...depend on compatibility of four-base overhangs flanking each genetic part. (A) Example Level 0 assembly...Level 0 vectors depends on the four-base overhangs flanking each part, as they need to match sequentially ...and request your sequence with the appropriate flanking restriction sites and overhangs already added....

...chat is a convenient alternative for asking a quick question or making initial contact, but is less suitable...to look forward to in a technical support role Talking to customers everyday, it can be fascinating and...right place to live it out, but ideally without asking too many (annoying) questions. Identifying and ...understanding of when to use these communication skills by working with people from many different backgrounds and... other roles in industry. Many thanks to Eric Perkins and Stephanie Hays for images used in this post...

...modulating the production of the pro-inflammatory cytokine interleukin IL-1 beta in the dorsal raphe nucleus (DRN... circular corridor apparatus using a camera or tracking software. Locomotion is a highly complex process...reflect elements of drug addiction and craving (‘seeking drugs’).  What’s involved in locomotor sensitization...protected) rather than being out in the open (risky). Taking advantage of these characteristic traits, rodents...reduced anxiety levels and a higher tendency for risk taking. These animals also show signs of neuroinflammation...

...consider a dual-sgRNA system. This system uses a “nicking” Cas9, in which one of Cas9’s two critical enzymatic...deleting the catalytic residue of a protein or knocking out a very small peptide. In these cases, you ...xCas9 has a PAM requirement of NG, GAA, or GAT, making it a more flexible cutter with the added bonus ... You can achieve your precise edit without even making a break.  Base editors are dead Cas9 (dCas9) or...sites was sufficient to repress transcription by blocking its initiation. In mammalian cells, robust repression...interest. dCas9-based repressors are useful when knocking out a particular gene isn’t feasible. For example...

..., 2020.   Scientists around the world have been making major improvements to CRISPR technology since its...blue light is readily absorbed and scattered by the skin, so it is not able to reach deeper tissues. Additionally... however, can penetrate 5 mm past the surface of skin and is less phototoxic. Using the FAST system, Ye...By fusing the two pdDronpa domains to the sites flanking the DNA-binding cleft of Cas9, the fluorescent...turn on transcription, label chromatin, and study kinetics. Using chemistry to photocage CRISPRs The aforementioned...spatial control of Cas9 cleavage when using a photomasking technique. Last, Hemphill et al. presented data...techniques (Matony et al., 2020). Whether you are looking to activate, repress, or modify a gene, you now...

...politics? Science is an apolitical process for seeking knowledge. The process begins with a testable hypothesis...experiments can help support or reject a hypothesis. Looking at the data, scientists then revise their hypotheses...scientists who hold a doctorate degree, are currently working full-time, and have received a research grant in... of the new administration’s actions, including taking down the climate change page on whitehouse.gov,...depending on the number of people supporting it.  3) Linking scientists to one particular political party while...politicians and citizens to include science in decision making, it will be considered a success. Below are resources...

...model organism in scientific research. Yes, the same kind we use in breads and other baked goods! Yeast is... the study of neurological diseases, such as Parkinson’s disease (PD). PD is primarily characterized by...C. elegans are also self-fertile hermaphrodites making it easy to conduct large experiments over multiple...60% of human genes have an ortholog in C. elegans making it ideal to study basic molecular biology processes...resources check out Addgene’s Worm Expression page. Looking for more model organisms? Check out Part two in...From the baker to the bedside: yeast models of Parkinson's disease." Microbial cell 2.8 (2015): 262. PubMed...

...without restructuring. Positive Points for Trainees - Taking Steps to Achieve a Successful Career Career Advice...various career opportunities through our career, networking, and management for scientists blog series. We...Session focused on finding this information and making it more easily digestible/available. What I viewed...welfare of the many researchers out there who are making great progress on some of the world’s most profound...At Addgene we hope that all parties involved in making academic research a successful and enjoyable path...Blog Post Read Our Career Blog Posts Read Our Networking Blog Posts ...

...delighted to see enterprising early career scientists taking an active part in creating their own opportunities...opportunities for career development and networking. Aside from the importance of the topics to be discussed, ...building valuable skills and meeting new people by working together on the event planning and execution. I...seeing many similar examples of science trainees taking a proactive role in their non-bench training. Grad...all. For grad students and postdocs interested in taking matters into their own hands, there are some easy...

...you are studying the effect of a new tau protein kinase inhibitor on slowing diseases progression in an...group of mice, the experimental group, with the new kinase inhibitor and a second group, the positive control...control group, with an alternative but well-studied kinase known to prevent tau hyperphosphorylation. You ...experimental protocol but does not receive either protein kinase inhibitor. Since the negative control group does...variability. If one were studying the effects of tau kinase inhibitors on mice, for example, it would be better...noise. This can be done either by modifying the blocking conditions or by substituting for a more specific...

...RNase free” and refrain from talking to friends and colleagues while working with these reagents. Other ...they are relatively small and easy to work with, making them the go-to choice for studying mammalian biology...technique is still in need of improvement. Also when working with CRISPR, one must beware that not all gRNAs...blog post. Finally, always remember that you are working with RNAs, which are prone to degradation by the..., Nakauchi H (2018) Intra-embryo Gene Cassette Knockin by CRISPR/Cas9-Mediated Genome Editing with Adeno-Associated...

...honing your science communications skills and networking. And on social media, you could be sharing your...Science Communications and Marketing Coordinator Networking “Many, perhaps most of the most interesting jobs...have to create opportunities. If you find yourself asking, "Why doesn't this product/organization/position...position and my team at Addgene was created.”- Eric Perkins, Director of Science Product Management “I knew.... Usually when I see inspiring artists who are thinking about getting into the arts (or any career to ...

... communicate effectively is part of what makes working in a team productive. Take all the opportunities...conferences on interviewing, resume writing, and networking Practice your English if you need to, go to ESL... a content-useful website, write a blog Pursue speaking invitations by inviting others (be on a speaker...“learn the business” Attend events with local networking and trade organizations Another excellent perspective...People Wish They’d Learned Sooner by Peggy Klaus Looking for a mentor or want to start a peer mentoring ...

...offers all of those same advantages to researchers working in plant model systems. “The cloning is all done...trade Lohmann does have one special tip for anyone looking to try this at home in their own lab: be very careful...along at 99 percent efficiency, simply stopped working. They finally traced the problem back to the endonuclease...in the process. “We had several motivations for making GreenGate available through Addgene,” Lohmann said...system, the more building blocks will be available, making it easier for us to do our work.” References 1...

... Something that I struggle with as I’ve been thinking about my career options is being an overthinker...overthinker. Being an overthinker can make me scared of taking risks, afraid of leaping forward but I have learned...in a life sciences career but life has a way of making unexpected decisions. As someone who was not necessarily...what my Instagram posts would look like. While working remotely was a different experience, It was helpful... to their roles at Addgene. I had a great time speaking with Quintin Marcelino who is Addgene’s Science...

...Mentor – what do I do now? What are the benefits of taking on my own Mentee? Q1. What makes a good science...participants had certainly realized the importance of seeking out mentoring as an important aspect to their ongoing...Mentor is often one that devotes time and energy to thinking about how to be a great Mentor.    Check Out Angela...on the blog “Tenure, She Wrote” is titled, “What Kind of Mentor Do I Want To Be?” In this excellent essay...the northeast, writes a thoughtful treatise on the kind of advisor/mentor she wants to be for her new lab...