We narrowed to 662 results for: Clu

...specific blockers, and the manufacturer will usually include recommended blocking conditions on the spec sheet...especially important for highly expressed proteins, including “housekeeping” or ubiquitously expressed proteins... to improve binding and/or reduce background, including trying longer incubations at both temperatures...

... Last year, we distributed 165,000 plasmids, including 27,000 CRISPR plasmids and 6,000 biosensors plasmids...antibodies. Everything in this collection, which includes both neuroscience and general-use antibodies, ...

...other uses.   Cloning grade DNA Molecular cloning includes a set of techniques that are used to insert recombinant...this agent to increase transfection success.  In conclusion, it is important for you to establish in advance...

...you run a restriction digest on a gel you always include proper controls like uncut DNA and the proper ladder...amplification reaction (as opposed to using the all inclusive service some companies offer), you can purify ...

...engineering research. A variety of new tools came out including the single base editors, Casilio, CombiGEM, and...2A-Puro (PX459) V2.0: What's not in the name? Not included in this plasmid's name are some additional features...

...organization runs a wide variety of programs, including fall and spring lecture series, science cafes,... information about SITN: DayCon event website (includes archived content) Science in the News website ...

...Start something (a journal, a science lunch club, a biotech club) Be the head of a group (like a grad student...

...that introduced the kit in December, GreenGate includes the six most frequently used elements in plant...works, just trash it.” Lohmann and his colleagues including Joachim Forner now expect and hope that other ...

... lentiviral packaging and expression vectors, including pMD2.G and psPAX2. Actually, these two plasmids...years after they were added to the repository, including beta-catenin reporter M50 Super 8x TOPFlash, scramble...

...on previously described pFA6a-link vectors and include a Kan, SpHIS5, or CaURA3 selection marker. Lee ... variety of genome modifications in S. Pombe, including full and partial gene deletion, overexpression...

...scientists in 88 countries around the world. That included 2008 pooled libraries, 290 antibodies, 18,359 ...it more accessible. Other accessibility updates include a printer-friendly design and alt text on all images...

...page, and a lot of the content was either small, cluttered, or hard to find. This made the page look not ... place all of the content above the fold, we decluttered the page elements and allowed our content to ...

...mentoring and applauded the new requirement for PIs to include in their annual NIH progress reports information... research be? The second day of the conference included workshops which were framed in advance by the ...

...met the astronaut Jim Lovell I was speechless (the club of people who have been in space is pretty small...Doudna has had a host of other impressive mentors including Tom Cech, Robert Tijian, Tom Steitz and Joan Steitz...

...colleagues built a Molecular Expressions collection including some 3,300 plasmids along with image galleries...specific cellular structures.The Davidson lab even included some of their own reference images that can be...

...available in the Addgene viral vector repository! This includes the first Addgene prep packaged in the AAV9-X1.1...have a bunch of other new AAV vectors as well — including next-generation biosensors for GABA, dopamine,...

...tags, often called polyhistidine tags, typically include six consecutive His residues (or more). Histidine... tags There are many different types of tags, including epitope tags (FLAG, Myc, etc.) and fluorescent...

... A free, donation-based plasmid analysis tool including editing, annotating, creating maps, and more. ...  Expasy: Bioinformatics resource portal which includes both bioinformatics tools and knowledge databases...

...biosensors as an example. Many calcium biosensors include GFP fused to calmodulin (CaM) and the chicken myosin... (ER), vacuole or cellular membranes. Examples include blue flame mitochondria-targeted calcium sensor...

...affected. Previous tools to overcome this restraint include an AAV-based sparse labeling system where a limiting...recombinase can work in other model organisms including mouse and fish, SPARC is likely generalizable ...

...Acoustic Reporter Genes (ARGs) are engineered gene clusters that encode proteins that form bacterial gas vesicles...have hollow interiors and a protein shell that excludes water but is gas permeable. These vesicles are...

...receptors that play many roles in signaling processes including smell, taste, sight, inflammation, and neurotransmission... could be measured using the TRUPATH system. Conclusions Although the importance of GPCR pathways is well...

...members of the ubiquitous activin ligand family, including Activin A. A commercially developed therapeutic...analytical development. His areas of expertise include early stage drug discovery and pre-clinical development...

...appreciated and valued This often stems from being included in organizational decision-making. It is imperative...things, diversity of responsibilities, which might include training or teaching others, research, and policy...

...technological biological blockbuster known as the CRISPR (clustered regularly interspaced palindromic repeats) Cas...in E. coli in 1987 (1), the concept that these clustered repeat sequences operated as a safeguard against...

... to do the research itself. Several years of exclusively writing lab reports and empirical essays had ...of those entering science communication (myself included) come from a scientific background and bring with...

...and is based on the pTRC99a plasmid. It does not include the PBAD promoter. If you already have a pBAD33...the plasmids deposited from Di Ventura's lab, including the popular light-inducible nuclear export system...

...Would you buy and use a plasmid if it didn’t include any plasmid sequence data? Of course not. Unfortunately... plasmid-based and offer numerous advantages, including more secure archiving, unambiguous molecular definition...

...control Historically, AAV vector QC has been sparse including only titration of AAV particles and a protein ... the expression cassette that we want, it also includes a small amount of spurious DNA from the packaging...

...but suffer from several important shortcomings including low processivity (ie. the probability of the polymerase...transport from the cell surface. Their toolkit includes a set of plasmids each containing an anti-GFP ...

...module. As a general guideline, experiments that include growing plants require pots, trays, soil, fertilizer...efforts. Our education partnerships have grown to include local school districts, professional education ...

...standardized methods for evaluating CRISPR technologies, including the ever important on/off-target efficiency. Likewise...guest bloggers Dominik Paquet and Dylan Kwart, including a detailed look at how cut-to-mutation distance...

...found in bacteria. Some common ones you might see include ColE1, pMB1 (which comes in a few slightly different...questions to get started: Will the plasmid be used exclusively in E. coli? Gram negative bacteria in general...

...association, started a speaker series or biotech club, managed their own blog or podcast, initiated and...future and the 1:1 format is good for everyone including those that find events stressful. Video chatting...

...number of unique features compared to Cas9. These include: More targeting flexibility Unlike Cas9 and the...base excision would alter the target sequence and preclude future HDR, which could lower HDR efficiency. ...

...napkin for paper, a small group of scientists including Stanley Falkow, Stanley Cohen, Herbert Boyer, ...eBook. Some highlights of the 2nd edition eBook include: new chapters on genome engineering, cloning, and...

...month.  We are currently focused on two projects, including one led by a talented biomedical engineering team...still able to get through to a number of experts, including Harvard/MIT faculty, venture capital and angel...

...the specificity of their luciferase assay, they concluded that components in the buffer used for protein...works in multiple types of primary mouse cells, including neuronal and gut stem cells, dendritic cells, ...

...multidirectional control, a new study repurposes the sgRNA to include modular synthetic RNA scaffolds that can recruit...RNA in the CRISPRi system can be repurposed to include modular RNA scaffolds. These RNA scaffolds can...

...creative thinking Enhance leadership skills Support inclusion, diversity, and open communication Feel valued...download the "Mentoring for Scientists" ebook, which includes the entire series of mentoring blog posts, the...