We narrowed to 640 results for: Son

...that contains sequence complementary to the dsRNA (Wilson and Doudna, 2013). RNAi consists of two non-exclusive..., Deans RM, Li A, Bassik MC (2016) Systematic comparison of CRISPR/Cas9 and RNAi screens for essential...:dev186452 . https://doi.org/10.1242/dev.186452 Wilson RC, Doudna JA (2013) Molecular Mechanisms of RNA...

...commonly used for recombineering can be found in Thomason et al and Sharan et al (a few strains can also...ecosalplus. PubMed PMID: 27223821. 4. Sharan SK, Thomason LC, Kuznetsov SG, Court DL. Recombineering: A ...19180090. PubMed Central PMCID: PMC2790811. 5. Thomason, L. C., Sawitzke, J. A., Li, X., Costantino, N...

...P. C., Shmakov, S. A., Silva, C., Tolstoy, I., Johnson, D. B., Koonin, E. V., & Quatrini, R. (2021). Evolution...https://doi.org/10.1093/nar/gkz1197 Samai, P., Pyenson, N., Jiang, W., Goldberg, G. W., Hatoum-Aslan, ... Hallmark, T., Keiser, D., Bondy-Denomy, J., & Jackson, R. N. (2021). Positioning diverse Type IV structures...

... is FRET and what's it good for? Fluorescence resonance energy transfer (FRET) is a biophysical interaction... excited, it can transfer energy directly via resonance to the second fluorophore (the acceptor) if the...PMID: 17512008. 4. Fessenden, James D. "Förster resonance energy transfer measurements of ryanodine receptor...

...heteroduplex transitions back to DNA duplex, and it’s the reason guide sequences have a maximum length of 20 bases...Sternberg et al., 2015).     Figure 8: Comparison of Cas9:RNA crystal structure (left, PDB ID 4ZT0..., Dangerfield, T. L., Jung, K., McCool, R. S., Johnson, K. A., & Taylor, D. W. (2022). Structural basis...

...for cloning but PX330 only uses 20 nt? A16: The reason for the 30 nt region on the upper is because the...doi.org/10.1126/science.1231143  Elliott, B., Richardson, C., Winderbaum, J., Nickoloff, J. A., & Jasin...cells differing exclusively at two early onset Parkinson Point mutations. Cell, 146(2), 318–331. https:...

...how Nextflow helped us manage our data. To quote Jason Niehaus, Director of Data Science and head of our...  Rachel Leeson...

...repositorio. Este increíble regalo permitirá que más personas accedan a recursos útiles para sus experimentos... Rachel Leeson...

...Undergraduate Research Experiences (CUREs)Author: Jason PellettieriNew Acoustic Reporter Genes: Ultrasound...  Rachel Leeson...

... research, for decades. Its short life cycle, reasonably close genetic homology to humans, and small size... Rachel Leeson...

...today✂️❄️🦠thanks to the awesome templates by @CVRHutchinson @CVRinfo. Check them out: https://t.co/uDEE9PsspD...  Rachel Leeson...

... of holiday spirit still linger (despite the unseasonably warm weather we had last weekend). We’ve spent...DeckTheLab. Getting in the spirit for the holiday season. Santa frog is coming to town. @Addgene @UMich_...

...think of any better way to start off our holiday season than by thanking the twelve guest bloggers and ...  Rachel Leeson...

...Addgene. A team of undergraduate students led by Dr. Song Tan at Penn State developed the plasmids, pPSU1 ...1000 Lanes Cost $200-$500  <$10 $20-30 Cost Comparison: While convenient, commercial ladders cost between...

...Here at Addgene, we’re always inspired by the new seasons, so we've made some big summer plans this year!...                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      Rachel Leeson...

... to #DeckTheLab with virus snowflakes from @CVRHutchinson #SciArt #STEAM #WinterWonderlab @Addgene Make...We hope everyone has a safe and festive Holiday season. 🙌 🥂 🎄 🎉 😷 Remember wear a mask,↔️social distance...

...easier to generate mouse models.   Cpf1 Update: Comparison to Cas9 and NgAgo As you might expect given the...Generating Mouse Models Using CRISPR/Cas9 Cpf1 Update: Comparison to Cas9 and NgAgo Special Delivery: Fluorophore...

... in the Collins' lab 6:19 - 8:26 : Dr. Koeris' reasons for wanting to develop bacteriophage for use as... - 32:20 : What did It mean for Dr. Koeris to personally take on the risk of founding a startup company...

...introduction to plasmids with Director of Biology Lianna Swanson was very popular, with the students excited to ...different career options in science, with Abigail Anderson commenting that “There is not just a scientist...

...of nanograms or micrograms of starting material; Gibson assembly or Golden gate assembly use a more direct...quality and outcomes of experiments. For these reasons commercially available kits provide extra washing...

... protein that binds an alternate sequence. By comparison, Abil explained, the specificity of other RNA...less reliable results. She also notes that for reasons that are not yet entirely clear some PUF proteins...

...honest feedback to the manager for a variety of reasons. They may feel that giving feedback will be perceived... to be too busy to be receptive.   Whatever the reason, it is a fortunate and successful manager that ...

...College of Medicine and Gordon Mills’s lab at the MD Anderson Cancer Center tackled this problem by developing...and large-scale patient cohort data from the MD Anderson Cancer Center patient databases, this research...

...from the Michael J. Fox Foundation are used in Parkinson’s research.  The extensive resource from the Structural...SLCs) for medical research Recently, the Michael Davidson Fluorescent Protein collection, a ‘One-Stop Shop...

... core branding. Blugene needs to be friendly, personable, fun, and promote our values of community and... Jason Snair...

...advantageous. This new technique, developed in Thoru Pederson’s lab, brings us one step closer to mapping the...Naseri A, Reyes-Gutierrez P, Wolfe SA, Zhang S, Pederson T. Proc Natl Acad Sci U S A. 2015 Mar 10;112(10...

...our resident CRISPR experts) at the 2014 Johnson & Johnson’s Dr. Paul Janssen Award dinner which took ...

...follow their passions, be happy, and match their personal goals to their career choices.” She points out...hard work, collaboration, and staying honest with personal goals. Stay tuned for the follow up blogs on "...

...Verkhusha popping up again and again, and for good reason. We all know scientists have used fluorescent proteins... higher resolution images in vivo. “One of the reasons we can’t localize one cell at a depth of one centimeter...

...approached me and asked about my current projects and personal interests. We chatted about social media analytics... being genuinely interested in knowing you as a person, builds a strong sense of trust between coworkers...

...monitored genes and mutants - viral integration, transposon, and many more! Check out Addgene’s catalog to...resources on the Addgene blog Plasmids 101: Using Transposons in the Lab Plasmids 101: Screens vs. Selections...

... with multiple serotypes pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato] AAV1 Optogenetics Edward Boyden New ...resources!   This blog post was written by Rachel Leeson and Brian O'Neill.  More resources on the Addgene...

...Covid gene, a toxin, or an oncogene, for safety reasons, and the insert size should be a max of 500 bp ...biochemistry in college and play the drum set as their personal instrument. They are the oldest sibling in a Guatemalan...

... we love camelids too, though not just for the reasons the UN describes. In honor of the Camelid family...Arbabi-Ghahroudi, 2017)!      Figure 1: Comparison of a heavy-chain only antibody and single-domain...

... and then 75% of the time I’m kind of the go-to person for my department.” Caitlin does this by acting... Keown, a staff writer at the non-profit Fred Hutchinson Cancer Research Center. And make sure to subscribe...

... school, and high school.  I’m the only science person of the bunch. They don’t really know where I came... you how to design a course, how to design good lesson plans, how to design test questions, and how to...

...experimental problems, legal restrictions, or any other reason. But this way, as soon as we become aware the related...toast to the new paper (or after — hey, another reason to celebrate!), send us a quick email at deposit...

...have popular plasmids but lack the resources or personnel to constantly prepare them for distribution. One...for sharing your plasmids at Addgene. You are the reason we’re able to do what we do!          Special ...

...Flexible work schedule Flexibility to manage their personal lives to achieve a healthy work-life balance. ...new workplace if opportunities for learning and personal growth do not exist or if they feel underutilized...

... and then applying this mixture to your tissue (Tuson, et al., 1990; Goodpaster, et al., 2013). One issue...983. https://doi.org/10.1177/002215549804600813  Tuson JR, Pascoe EW, Jacob DA (1990) A novel immunohistochemical...