...Addgene. A team of undergraduate students led by Dr. Song Tan at Penn State developed the plasmids, pPSU1 ...1000 Lanes Cost $200-$500  <$10 $20-30 Cost Comparison: While convenient, commercial ladders cost between...

...Here at Addgene, we’re always inspired by the new seasons, so we've made some big summer plans this year!...                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      Rachel Leeson...

... to #DeckTheLab with virus snowflakes from @CVRHutchinson #SciArt #STEAM #WinterWonderlab @Addgene Make...We hope everyone has a safe and festive Holiday season. 🙌 🥂 🎄 🎉 😷 Remember wear a mask,↔️social distance...

...easier to generate mouse models.   Cpf1 Update: Comparison to Cas9 and NgAgo As you might expect given the...Generating Mouse Models Using CRISPR/Cas9 Cpf1 Update: Comparison to Cas9 and NgAgo Special Delivery: Fluorophore...

... in the Collins' lab 6:19 - 8:26 : Dr. Koeris' reasons for wanting to develop bacteriophage for use as... - 32:20 : What did It mean for Dr. Koeris to personally take on the risk of founding a startup company...

...introduction to plasmids with Director of Biology Lianna Swanson was very popular, with the students excited to ...different career options in science, with Abigail Anderson commenting that “There is not just a scientist...

...of nanograms or micrograms of starting material; Gibson assembly or Golden gate assembly use a more direct...quality and outcomes of experiments. For these reasons commercially available kits provide extra washing...

... protein that binds an alternate sequence. By comparison, Abil explained, the specificity of other RNA...less reliable results. She also notes that for reasons that are not yet entirely clear some PUF proteins...

...honest feedback to the manager for a variety of reasons. They may feel that giving feedback will be perceived... to be too busy to be receptive.   Whatever the reason, it is a fortunate and successful manager that ...

...College of Medicine and Gordon Mills’s lab at the MD Anderson Cancer Center tackled this problem by developing...and large-scale patient cohort data from the MD Anderson Cancer Center patient databases, this research...

...from the Michael J. Fox Foundation are used in Parkinson’s research.  The extensive resource from the Structural...SLCs) for medical research Recently, the Michael Davidson Fluorescent Protein collection, a ‘One-Stop Shop...

... core branding. Blugene needs to be friendly, personable, fun, and promote our values of community and... Jason Snair...

...advantageous. This new technique, developed in Thoru Pederson’s lab, brings us one step closer to mapping the...Naseri A, Reyes-Gutierrez P, Wolfe SA, Zhang S, Pederson T. Proc Natl Acad Sci U S A. 2015 Mar 10;112(10...

...our resident CRISPR experts) at the 2014 Johnson & Johnson’s Dr. Paul Janssen Award dinner which took ...

...follow their passions, be happy, and match their personal goals to their career choices.” She points out...hard work, collaboration, and staying honest with personal goals. Stay tuned for the follow up blogs on "...

...Verkhusha popping up again and again, and for good reason. We all know scientists have used fluorescent proteins... higher resolution images in vivo. “One of the reasons we can’t localize one cell at a depth of one centimeter...

...approached me and asked about my current projects and personal interests. We chatted about social media analytics... being genuinely interested in knowing you as a person, builds a strong sense of trust between coworkers...

...monitored genes and mutants - viral integration, transposon, and many more! Check out Addgene’s catalog to...resources on the Addgene blog Plasmids 101: Using Transposons in the Lab Plasmids 101: Screens vs. Selections...

... with multiple serotypes pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato] AAV1 Optogenetics Edward Boyden New ...resources!   This blog post was written by Rachel Leeson and Brian O'Neill.  More resources on the Addgene...

...Covid gene, a toxin, or an oncogene, for safety reasons, and the insert size should be a max of 500 bp ...biochemistry in college and play the drum set as their personal instrument. They are the oldest sibling in a Guatemalan...

... we love camelids too, though not just for the reasons the UN describes. In honor of the Camelid family...Arbabi-Ghahroudi, 2017)!      Figure 1: Comparison of a heavy-chain only antibody and single-domain...

... and then 75% of the time I’m kind of the go-to person for my department.” Caitlin does this by acting... Keown, a staff writer at the non-profit Fred Hutchinson Cancer Research Center. And make sure to subscribe...

... school, and high school.  I’m the only science person of the bunch. They don’t really know where I came... you how to design a course, how to design good lesson plans, how to design test questions, and how to...

...have popular plasmids but lack the resources or personnel to constantly prepare them for distribution. One...for sharing your plasmids at Addgene. You are the reason we’re able to do what we do!          Special ...

...Flexible work schedule Flexibility to manage their personal lives to achieve a healthy work-life balance. ...new workplace if opportunities for learning and personal growth do not exist or if they feel underutilized...

...thought about how X works?”, or even by introducing a person that the audience can relate to. It doesn’t need...  Rachel Leeson...

...monoclonal antibody production (Oyaas, 1989; Ozturk and Palsson, 1991; Oh, 1993) and increased yields in adenoviral...272-278. 4. Ozturk, Sadettin S., and Bernhard O. Palsson. "Effect of medium osmolarity on hybridoma growth...

...Cele S, Gazy I, Jackson L, Hwa S-H, Tegally H, Lustig G, Giandhari J, Pillay S, Wilkinson E, Naidoo Y, Karim...

...Bradbury ARM, Trinklein ND, Thie H, Wilkinson IC, Tandon AK, Anderson S, Bladen CL, Jones B, Aldred SF, ...

...communication (it is, after all, how one moves from layperson to expert) and peers can and do consume science...  Rachel Leeson...

...mentoring groups seem to be as successful as in person groups. Maybe it’s just that it’s easier for everyone...A How-To Guide for Scientists.   Once in-person, peer-mentoring groups at Addgene have gone virtual...

... were excited to deliver the very first ones in person by our own Tech Transfer Team! Why MTAs Matter ...Laboratory  Dana-Farber Cancer Institute  Fred Hutchinson Cancer Research Center Memorial Sloan-Kettering...

... and then applying this mixture to your tissue (Tuson, et al., 1990; Goodpaster, et al., 2013). One issue...983. https://doi.org/10.1177/002215549804600813  Tuson JR, Pascoe EW, Jacob DA (1990) A novel immunohistochemical...

...podcast segment Similarly to the Carr lab, Erik Sontheimer and his group from the University of Massachusetts... surrounding the dSpyCas9-APEX2 fusion.  This Sontheimer lab used this technology, which they call C-BERST...

...multitude of methods including surface plasmon resonance (Biacore, GE Life Sciences) or bio-layer interferometry...protein on blots with Streptavidin-phosphatase (Jackson ImmunoResearch, 016-050-084; 0.2 μg/ml final). ...

... of your target organism. In this post, Chris Richardson, a postdoctoral researcher in Jacob Corn’s lab...this post, Albert Cheng and Mark Wanner from the Jackson Laboratory for Genomic Medicine described Casilio...

...Lederberg's Personal Perspective: Plasmid (1952-1997) Cohen, Stanley N. “DNA Cloning: A Personal View after...

...founding and running miLab to describe some key lessons I learned while becoming a health science entrepreneur...cold email Outside of the potential contacts your personal network can offer, you are really on your own ...

...from  science-focused to business-focused?" The reason is that I knew that the mind-set of the employer...instead I thought they would focus on “Has this person been consistent throughout their career?”, "Have...

...Just getting together and chatting with another person about their career path is a great start to a mentoring... read before your meetings and discuss these in person. See the extensive resource list available from...