We narrowed to 697 results for: Tec

...that can be tweaked to target ncRNA. CRISPR/Cas9 techniques and targeting methods Knock-out: Targeting short...targeting of long non-coding RNA genes. Nature biotechnology. 2020;38(5):573. doi:10.1038/s41587-020-0428... cells by Cas9 targeting of splice sites. Nat Biotechnol. Published online November 5, 2018. doi:10.1038...Non-Coding RNAs in Plants with the CRISPR-Cas Technology is a Challenge yet Worth Accepting. Front Plant...

...Susanna Stroik Even with the advances in CRISPR technology over the past decade, primary cells remain difficult...using peptide-assisted genome editing. Nature Biotechnology, 10.1038/s41587-023-01756-1. https://doi.org...monomeric StayGold fluorescent protein. Nature Biotechnology, 1–4. https://doi.org/10.1038/s41587-023-02018...throughout the non-human primate brain. Nature Nanotechnology, 18, 1241–1251 (2023). https://doi.org/10.1038...

...molecular biologic techniques to further the understanding of epigenetics; techniques include chromatin...and bisulfite sequencing. However none of these techniques can be used for in-situ research of dynamic modifications...reporting methylation status of these sites. This new technology opens up a new way to visualize changes in gene...

...variety of research sectors to provide open source technologies and spaces where interested parties can do research...fantastic optics and fluidics platform it contains. Biotech equipment becomes obsolete amazingly fast, but ...is a passion for learning and having fun with technology, so feel free to contact your local space and...Biocurious.org, Derek is bringing cutting edge technologies to Victoria, BC. Follow Derek on Twitter @derekja...

...   Novel protein cage provides simple enzyme protection in yeast bioproduction pathways By Gabrielle ...(MPyV VLPs ). This artificial nanoparticle can protect proteins from unfavorable interactions in the cell...pathway (myo-inositol oxygenase), providing enzyme protection while still allowing substrates and products ...system provides a simple, efficient method for protecting a single enzyme that drives biosynthetic pathways...

... – so much so that there was little range for detectable improvements in the methodology. To further push... While T4 DNA Ligase, the mainstay of most biotechnological cloning efforts for over 50 years, prefers...work, will fuel additional efforts to push the technique forward. Many thanks to our guest bloggers Becky... efficiency Learn about other plasmid cloning techniques Resources on Addgene.org Find basic molecular...

...many labs still love using them! With so many technologies currently available, you now face the challenge...Alternatively, you might consider Gibson cloning. In this technique, you first perform a round of PCR to generate ... there are never any guarantees, some cloning techniques do tip the scales in your favor. For starters...ligation-independent PCR cloning method." BMC biotechnology 11.1 (2011): 92. PubMed PMID: 21992524. PubMed...

...sequencing capabilities and Seqwell’s plexWell technology, we can now perform NGS at Addgene to sequence... we mixed into the original AAV sample at 5%. Detecting 5% contamination is a good start, but to determine..., with a high enough sequencing depth, we could detect contamination as low as 0.1% (Guerin et al., 2020...perform a deeper analysis of the data. Our serotype detection software confirms the serotype of the prep by ...

... low, ranging from undetectable to 0.1%. In contrast, new genome editing techniques such as CRISPR are...the endogenous gene. Here, we'll describe the techniques and the plasmids used to inactivate specific ...specifically with a gRNA). Be sure to keep these techniques in mind when choosing a knockout strategy! Knockout...

...and in the right places. Checks and balances: protecting against cancerous cells Luckily, there are several...several safety mechanisms in place to protect us from rogue cells when something does go awry. The process... use with the Gateway Cloning Platform (Life Technologies, Carlsbad, CA) that represents 180 genes from...Scientists are making progress. New resources and technologies become available almost daily, yet obstacles...

...out of fear it points to flaws or gaps in their technical ability. But, especially for AAV data, that may...experimental settings. It is a curated set of technical reports on AAV vectors from Addgene and the CLOVER...evaluate AAV vector performance. Yet, most of the technical data needed to replicate and build on others’ ...word of mouth” as their source for this type of technical know-how, which highlights the dearth of AAV vector...

...a multi-spacer CRISPR array. Workflow and technical considerations Making insertions in bacterial ...metabolic cassettes (Zhang et al. 2020); however, the technology potential enabled by CRISPR-transposons has yet...CRISPR biology and CRISPR genome engineering technologies. Message or follow him on twitter @Vopinator...multiplexed bacterial genome engineering. Nat Biotechnol. https://doi.org/10.1038/s41587-020-00745-y Zhang...

... and the chromosome conformation capture (3C) technique. In addition to 3xFLAG and biotin tags, use of...additional flavors to this methodology. Using this technique, researchers have begun to make important discoveries...experimental setup. As we’ve seen for many other CRISPR techniques, this method also has great potential for improvements...DNA fragments and chromatin by in vitro enCh IP technology using recombinant CRISPR ribonucleoproteins."...

...clever chemists and biochemists have developed techniques to make labeling chemistry easier and more functional...the initial breakthrough for in-cell labeling techniques was provided by Roger Tsien, who showed that ... century. Many of the small-molecule labeling techniques have focused on this classic, net-neutral dye...inside cells with fluorescent tags." Trends in biotechnology 30.1 (2012): 8-16. PubMed PMID: 21924508. PubMed...

...predicted off-target sites, Cpf1 does not mediate detectable off-target cleavage. Most gRNAs directed low-... CRISPR–Cpf1 Using a Single crRNA Array." Nat Biotechnol. 35.1 (2016): 31-34. PubMed PMID: 27918548. ...specificities of Cpf1 endonucleases in human cells. Nat Biotechnol. (2016). PubMed PMID: 27272384 Find plasmids...CRISPR-Cas Cpf1 nucleases in human cells." Nat Biotechnol. (2016). PubMed PMID: 27347757. Find plasmids...

... many other uses currently exist, and new GFP technology is constantly being developed! Fusion tagging...GFP-based fluorescent biosensors has been designed to detect a variety of intracellular conditions, including...here. Optogenetics: Scientists can use light to detect, measure, and control molecular signals, cells,... articles and insight on the newest tools and techniques in fluorescent microscopy. Read More: All ...

...characterize, and develop new CRISPR systems and technologies, the AbuGoot lab often tests samples of new ...positions defined by the target:spacer duplex without detectable non-specific activity. Its major advantage is...same comparisons, the AbuGoot lab reported no detectable effect on cell viability – meaning there was ... genes. In comparison with other large insert techniques like HITI or HDR, which require specific repair...

...Therapy Consortium. Leveraging their combined technical expertise to develop a viable therapeutic, the... and Feng Zhang, PhD, is pursuing CRISPR/Cas9 technology for precise correction of Rett syndrome mutations...three neuronal subtypes of the hippocampus. This technology has formed the basis of the Rett syndrome program...co-founded by Gail Mandel. There are numerous technical challenges to overcome for both of these approaches...

... Mateo Sanchez and Alice Ting have used one biotechnology tool to optimize another. The authors have created...some notable improvements. Directed evolution techniques mimic natural selection of sequences that evolve... proteins at small and large scale.  This new technique involves selection in the cytosol (instead of ...purification required! The team used other molecular techniques to introduce time modulation of the TEV cleavage...

... induced gene silencing (VIGS) is a transient technique that uses a virus and the plant's post-transcriptional...surface) of the leaf. This is a simple and easy technique to implement. Success might vary between experimenters...Boston, MA. He obtained his BSc and MSc in Plant Biotechnology from Leibniz Universität Hannover (Germany),...PMID: 16739580. Li, Jian-Feng, et al. "The FAST technique: a simplified Agrobacterium-based transformation...

...into target genomes and a 10-fold improvement in detection efficiency over the original CROPseq. Find CROPseq-multi...the protein La (a small RNA-binding exonuclease protection factor) promotes editing (Yan, J et al., 2024...stabilizing the 3’ end of the pegRNA, which is not well protected by Cas9. This is similar to the proposed mechanism... effort to fill a void in available tools for detecting SARS-CoV-2 (Terry et al., 2021). Check out their...

...their expression to individual cells. With this technique, a Neuroscientist can see the full dendritic arbor... able to distribute materials containing FLEx technology. Read the genOway press release for more information...Recombinase Evolved by Cycling Mutagenesis.” Nature biotechnology 16(7): 657–62. PubMed PMID: 9661200.  3. Cardin...at the Cellular Level in the Mouse.” Nature Biotechnology 21(5): 562–65. PubMed PMID: 12665802. 9. Villa...

... the screening strategies outlined below, the technique can be used as a preliminary validation on your...Decomposition (TIDE) (Brinkman et al., NAR). This technique uses the trace file generated in Sanger sequencing...Sequencing Options for CRISPR Genotyping NGS and detecting off target effects  While pricier, NGS can be ...analysis. If NGS is not an option, you can still detect off-target effects with Sanger sequencing, provided...

...antibodies: the power of in vitro display technologies. Nat Biotechnol 29:245–254 . https://doi.org/10.1038...of Milstein and Köhler who developed hybridoma technology. But what about single chain fragment variables...living cells. The HA frankenbody is an HA tag detection probe. It contains an HA-binding scFv that’s fused...

...modifications feasible and even easy. This targeted-break technology coupled with HDR is a powerful site-specific ...the genome, resulting in off-target effects. A technique that pushes the size limitations of ssODNs is ...principles driving CRISPRs and genome editing technology. Links: Zhang Lab’s CRISPR FAQs CRISPR forum...asymmetric donor DNA. Richardson CD, et al. Nat Biotech. 34(3); (2016). PubMed. Efficient homology-directed...

... illuminates the passing cell with a laser and detects the light scattered by that cell at a low angle...laser beam and causes it to scatter (FSC/SSC), the detected photons can be represented through a graph of ... only enter dead cells, while living cells can protect themselves from it. So here, I actually want to...— describing the intensity and duration of the detected signal. Using a practical example of lymphocytes...

... events and training courses on technologies and laboratory techniques related to antibody discovery. ...) either produced de novo using yeast display technology or produced from hybridoma antibodies developed...synthetic rAbs, developed using yeast display technology, are directed to integrins that bind the Arginine-Glycine-Aspartate...

...: Čelešnik, Helena, et al. "Biosafety of biotechnologically important microalgae: intrinsic suicide switch..., Xuechen Zhu, and Charles E. Melançon III. "Detection and Quantification of Ribosome Inhibition by Aminoglycoside...fusions reported in the literature include RNA detection, RNA cleavage, and translational repression or...Genetically Encodable System for Sequence‐Specific Detection of RNAs in Two Colors."ChemBioChem 17.10 (2016...

...with antiviral therapies such that the virus is undetectable in the blood. But the virus doesn’t completely...respond. Latently infected cells evade immune detection because they produce very little or no viral protein...reinfection. Importantly, no off-target effects were detected. Kaminski et al. observed no negative effects ... field, the lessons learned in pursuing one technological application can benefit many others and accelerate...

... IMP USA-Europe not only provide the specific technical and logistic support to increase the chances of...of success, but also nurture a supportive and protective environment where individuals are empowered to...represented a diverse group of individuals with technical and career path needs, and came from the universities...Harvard University, Massachusetts Institute of Technology, Boston University, Johns Hopkins University,...

...Table 1: AAV Serotypes and Tropism Experiment  Detection Method CNS AAV1, AAV2, AAV4, AAV5, AAV8, AAV9...purposes. The University of Pennsylvania and the biotechnology firm Biogen have recently collaborated to develop...is their ability to be used with new exciting technologies (such as CRISPR/Cas9) as they allow for gene... fourth generation of neuroanatomical tracing techniques: exploiting the offspring of genetic engineering...

...and smaller engineered antibody fragments. One technique we haven’t discussed in depth is antibody directed...large mutant libraries, high-throughput screening techniques such as ELISA and cell surface display are common... in learning more about antibody engineering technologies, the Institute of Protein Innovation (IPI) provides...fragments and the rise of single domains. Nature Biotechnology, 23(9), 1126–1136. https://doi.org/10.1038/nbt1142...

...scientific team came as an undergrad at Worcester Polytechnic Institute. Though some WPI students chose to ... physical kits are made and shipped; Chiara, the tech transfer expert who understands the somewhat complicated...repository, performing quality control, and providing technical customer service. Team members are highly trained...a biostatistician, and that one golden-handed technician who can do a certain difficult assay with her...

...HaloTag or SNAP-tag construct can help you better detect dim structures or low levels of expression with...performance and use super-resolution or single-molecule techniques.   Figure 2: Two-color super-resolution...proteins with small molecules in vivo. Nature Biotechnology, 21(1), 1. https://doi.org/10.1038/nbt765  Lavis... V. (2008). HaloTag: A Novel Protein Labeling Technology for Cell Imaging and Protein Analysis. ACS Chemical...

... label it with finesse using tRNA suppression technology (Fig. 1) (Dumas, Lercher, Spicer, & Davis, 2015... from depositing labs that have mastered this technique so it might be worth a try, especially since the...References 1. Degorce, François, et al. "HTRF: a technology tailored for drug discovery–a review of theoretical...Time-Resolved FRET-Based Approach for Antibody Detection–A New Serodiagnostic Concept." PloS one 8.5 (2013...

...Addgene gave to me: SHERLOCK and DETECTR, two CRISPR-based methods to detect nucleic acids.  On the third ... CRISPR, Addgene gave to me: five methods for detecting SARS-CoV-2 using CRISPR.  On the sixth day of ...

... themselves. All of them have cool new tools, techniques, or information that is now available for other...demethylation. AAV Q&A with Tim Miles: While not technically a research story, I couldn't resist adding in...learning about it (and maybe even picked up a new technique or tool to try in your own work!) If you've got...

...particular microorganism, microbial technique, or microbial technology, sign up to write about it on the...CRISPR guide to find out how to use genome editing techniques in your microbe of choice. For more help with...

...using the streak plate technique. Time for the #BioSci3319 #StreakPlateChallenge part 1. Lab B needs a...Sciences at Missouri University of Science and Technology, has been teaching the microbiology lab every...

...CRISPR continues to grow as the genome editing technique has developed and improved. The CRISPR posts written... for more detailed information about this new technology. Our Plasmids 101 series continues to grab scientists...questions or if you have a topic, researcher, or technology you would like us to feature in the coming year...