We narrowed to 932 results for: Gene

...raffled off one of our highly sought-after "nice genes…" tshirts, and the lucky winner was Shagun Sharma...Developing the Addgene microbiology community #ASMMicrobe2016 was a great opportunity for Addgene to let microbiologists...materials! Resources on the Addgene Blog Learn How You Can Use Addgene's Microbiology Pages Read Our ...The start of the conference After setting up the Addgene booth on Thursday evening, I stayed to see the ...with lots of researchers who weren’t aware of Addgene’s plasmid sharing services, as well as #happydepositors...dedicated to science communication through art. Addgene Advisory Board member Siouxsie Wiles gave us a ... about amazing work already represented in the Addgene repository (like these new Rainbow Bacterial Fluorescence...

... in most cases at Addgene, we check what we call the “insert,” (the gene or genetic element cloned into...information for a given plasmid are the backbone, inserted gene/functional mutations, antibiotic resistance, restriction... Plasmid Learn How Addgene Uses Barcodes to Track Samples Resources on the Addgene Website View our ...the best things about sharing plasmids through Addgene is that we provide an added level of confidence... plasmid quality is to stay organized. Here at Addgene, we collect data on every plasmid we distribute...examples include Benchling, Serial Cloner, and Snapgene), and save these to a shared computer or server...physical sample and its associated information are at Addgene, we are ready to sequence verify the plasmids. ...

... researchers with many different ways to control gene expression. Get the full scoop from Addgenie A. ...how Cas9 homolog, Cpf1, may make it easier to make gene edits at new genomic locations. 6. CRISPR 101: Non-homologous...Adeno-associated viral (AAV) vectors are useful for in vivo gene delivery, but have limited packaging capacity making...2015 has been an exciting year for the Addgene blog. We’ve released over 80 posts on a diverse array ...have started providing recorded interviews with Addgene community members. In addition we’ve experienced...them a read if you’ve missed them! Breakdown of Addgene's top blog posts from 2015 Our most popular posts...your own guest posts by e-mailing us at blog@addgene.org. Thank you again for a fantastic year and happy...

...is in this subject.” Outreach travels with Blugene Blugene loves to visit researchers, so Outreach Scientist...Regional High School Resources at Addgene  Read more Inside Addgene Blog Posts Get Career Advice from...their day with breakfast and an introduction to Addgene with our Executive Director, Joanne Kamens. As ...to start networking! Learning about careers at Addgene Afterwards, the students split into two groups ...groups for an in-depth look at some of the teams at Addgene. An introduction to plasmids with Director of Biology...slideshow from our UK team member Benoit Giquel. Bluegene’s globetrotting impressed our guests, as did how...with the “comfortable surroundings” of the new Addgene office space.  Thanks Abigail, we think it’s pretty...

...this is to examine expression of the early response gene c-fos. For example, using Chr2 stimulation should...and standardization of genetic tools in the laboratory mouse, most optogenetics is performed in this species...Central PMCID: PMC3032981. Resources at Addgene A Primer on Optogenetics: Introduction and Opsin Delivery The...considerations you should think about when developing optogenetics behavioral experiments. There are far too many...many behaviors that have utilized optogenetics to be fully summarized in a short blog post, but some examples...ill-defined region of the brain. With the advent of optogenetics, ICSS can now be done with neuronal cell-type...the literature to find all the applications of optogenetics relevant to your field. Ultimately, your question...

... development of optogenetics, has genetically manipulated a number of the opsin genes to alter the light...have also been engineered to express various opsin genes [7-9]. The advantage of using these lines is, if...Regardless of the method of expression of your opsin gene, it is essential to test the expression of your ...PMID: 22470147.  6. Gore BB, et al. Manipulating gene expression in projection-specific neuronal populations...sections of optogenetics papers. Optogenetics step one: Deciding how you want to use optogenetics The most...things in science, optogenetics is best used to address a specific question. Optogenetics is a tool and, ...electrophysiology to prove that your optogenetic manipulations are generating action potentials (or lack there...

...Hey guys, Blugene here! As Addgene’s mascot, I’ve watched Addgene grow into the successful, internationally...to the entire Addgene family; the depositors and requesting scientists, all of the Addgene employees, the... the Addgene board members, vendors, Addgene supporters, and of course, Executive Director, Joanne Kamens...week, the Addgene 10-year Anniversary Gala celebrated all of the people who have helped Addgene grow and...changes at Addgene over the last 10 years as well as the many people that make up the Addgene family. It...Ken Fan each spoke about what Addgene means to them. Ken mentioned Addgene’s incredible growth and our determination...make copies of the newspaper, Addgene uses E. coli bacteria to generate copies of plasmids. The community...

...ribosome binding site (RBS) followed by a reporter gene. The hairpin blocks translation by sequestering ...reveals the RBS and allows translation of the reporter gene resulting in GFP signal. With the addition of standards...antibiotic resistance markers, promoters, and inducible gene expression systems in bee gut microbiome species...or preparation, and deliver CRISPR components for gene disruption or CRISPRi. While designed to study the...address these questions, biologists often use next-generation sequencing. Sequencing the 16S rRNA reveals the... also study the microbiome by using engineered genetic tools and reporter microbes. Let’s take a look ...microbiome with sequencing is vast, the ability to genetically alter microbiome members is limited and many ...

.... Human synapsin 1 gene promoter confers highly neuron-specific long-term transgene expression from an...pooled CRISPR knockout libraries) and individual gene knockouts (using gRNA lentiviruses). Separating ...performing a screen or even a single gene knockout. Top AAV: pAAV-hSyn-DIO-hM4D(Gi)-mCherry Our current...adult rat brain depending on the transduced area. Gene Ther. 2003 Feb;10(4):337-47. PubMed PMID: 12595892...Resources on Addgene.org Find Ready-to-Use Lentivirus Find Ready-to-Use AAV Catch up on Chemogenetics ...accelerate their work. After all, as we like to sayat Addgene, productivity is infectious. Curious which viruses...please)... Top Lentivirus: lentiCas9-Blast Most of Addgene’s lentiviral inventory is based on CRISPR tools,...

... PellettieriNew Acoustic Reporter Genes: Ultrasound Imaging of Gene ExpressionAuthors: Marjorie Buss and..., and that means it's officially the start of Addgene's winter holiday celebrations! We're kicking it ...Joachim Goedhart  Created in collaboration with Addgene writers High Complexity Golden Gate Assembly with...DADExperts: Matthew Norton and NEBGCE4All: Making Genetic Code Expansion AccessibleResearcher: Ryan Mehl;...big thank you to all who have contributed to the Addgene blog this year! Your contributions have helped ...

... your antibody is validated! Genetic Strategies This approach uses a gene knock-down or knock-out approach...all sample types are amenable to genetic manipulation, nor can all genes be easily knocked down or out. ...bioinformatician) to look at RNA expression of your gene of interest across a set of human tissues.  From...Conveniently, you found a cell line in which the gene for your protein has been mutated using CRISPR/Cas9...plasmid encoding your protein of interest, known as Gene of Interest (GOI), and transfect it into your favorite...this protein, which we will call Negative Control Gene (NCG) (A). Then you lyse the cells and run a Western... post!  Resources on Addgene.org Find plasmids encoding antibodies at Addgene Stay tuned for ready-to-use...

...Resources on Addgene.org Visit Our Microbiology Pages Use the FX Cloning Sytem to Clone Your Gene of Interest...making it difficult to quickly identify possible gene function. In this post, I will describe a useful...done, this technique can also help pinpoint the genes within a sequenced genome that encode for functional...digesting phage genomic DNA into 1-3 kb (approximately gene-sized) fragments so that these fragments can be ...results in optimal likelihood of capturing a whole RBP gene without causing issues associated with cloning large...back to the original plate to identify which phage gene encodes that protein! First, block your membranes...for a review, see Simpson et al., 2015). Visit Addgene's MIcrobiology Pages The problem: Host binding proteins...

...throughput studies of optogenetics.  Common opsins used in zebrafish Optogenetics functions through opsins....  Additional resources on the Addgene blog Find all our optogenetics blog posts Read blog posts about... viral vectors Subscribe to the Addgene blog Resources on Addgene.org Learn about our ready-to-use viral...Optogenetics, the use of light sensitive proteins (opsins) to manipulate cell activity, enables researchers...escape reflex. These traits, coupled with short generation times and high fecundity, make zebrafish ideal...are light sensitive proteins. Opsins used in optogenetic studies are not native to vertebrates and are...swimming  Researchers at the Isacoff lab applied optogenetics in zebrafish larvae to identify new neurons ...

...content from Addgene, subscribe to Addgene’s YouTube Channel. Additional resources on the Addgene blog Learn...about Addgene's Lab Tips video series Resources on Addgene.org Find lab protocols on the Addgene website...Since rolling out our viral service in 2016, Addgene has shipped thousands of AAV and lentiviral preps... purity of all AAV viral lots. Aligning with Addgene’s mission to “accelerate research and discovery ...improve your experiment results! Subscribe to the Addgene YouTube page! AAV purification by iodixanol gradient...calculations on how to prepare a plasmid stock used to generate a standard curve, visit the AAV Titration by qPCR...website Watch Addgene's video collection ...

...to highlight some of the top articles from the Addgene blog this year. Spoiler alert: many of the top ...subset of the fluorescent protein family and they generate color! They've been adapted for many molecular...SARS-CoV-2, including many with plasmids deposited at Addgene! 2. DNA Purification Without a Kit by Beth Kenkel...

...Subscribe to the Addgene newsletter for updates on all things Addgene; or subscribe to the Addgene blog for updates...request an AAV composed of an AAV plasmid in the Addgene repository and their choice of five different serotypes...comes to ordering high-quality materials from the Addgene repository. Read on to learn more.  What will be... be available?  Over 3000 AAV plasmids in the Addgene repository will be available for Packaged on Request...request. Request details can be accessed within your Addgene account. Preps will be available for request in...Once submitted, the request will be reviewed by Addgene for legal approvals and technical compatibility...Cart” button will appear with the request in your Addgene account.  Orders do not need to be placed immediately...

...We're going virtual for Addgene’s first AAV Data Hub Challenge, now taking place Dec. 6, 2022! Share ...Anyone can submit data from their CLOVER lab or Addgene AAV experiments to the Data Hub. Submit your data...travel award. All participants will receive an Addgene t-shirt.  Our judges, Drs. Connie Cepko, Ian Wickersham...

...a second plasmid will be needed to introduce the gene of interest containing the chosen codon that will...limiting. It may be time, then, to look towards genetic code expansion (GCE): techniques in which non-canonical...essentially those needed to make site-directed mutagenesis, many researchers new to GCE aren’t able to successfully...added phosphoserine. A community resource Like Addgene, GCE4All is a community resource which depends ...researchers. One way of doing that is by using Addgene’s Blue Flame award to identify highly requested ...the plasmids and bacterial strains (available at Addgene for GCE work. Plus, keep an eye on our blog – we...., Griffith, M. C., & Schultz, P. G. (1989). A General Method for Site-specific Incorporation of Unnatural...

...knockout screening, sgRNAs guide Cas9 to cut targeted genes, creating mutations that prevent their expression...expression. The knockout cells can be used to identify genes that underlie a particular phenotype. Figure...knockout screens: sgRNAs to guide Cas9 to targeted genes. In these applications, however, nuclease-dead Cas9...contain thousands or millions of different books. Addgene’s pooled libraries are a little different: each ...are currently 390 pooled libraries available at Addgene, with more added to the collection all the time...barcodes help librarians track individual books, genetic barcodes allow researchers to identify specific...technologies multiply, so do CRISPR pooled libraries! Addgene’s collection also includes a base editing sensor...

... time or location can be critical when, say, the gene is essential during development and a knockout is...the Addgene blog Viral Vectors 101: Optogenetics Additional resources on Addgene.org Optogenetics Guide... The Chow Lab has also generated a variety of membrane-recruitment optogenetic tools for manipulating ...a full toolbox and workflow for generating new Rho GTPase optogenetic systems (Berlew et al., 2022). Stopping...LOV, CRY2-CIB, and other optogenetic modules? Or want to find more optogenetic tools in our collection?...collection? Check out our Optogenetics Guide or browse our collections of optogenetics plasmids – and keep an eye...Optogenetics gives you the power to control cells or organisms with the flip of a switch. You may be ...

... is easy. Ordering genes on plasmids online is a little trickier -- but at Addgene, we try to make the...here at Addgene. If you’ve called our customer service line or sent an email to help@addgene.org, you’ve...seller. However, Addgene is a bit different from other vendors. As a nonprofit, Addgene does not own the... bigger question: How does a customer place an Addgene order using a PO number? Why do I have to order...distribute it on behalf of depositing institutions. Addgene cannot fulfill orders based on PO documents that...information that is entered at checkout is used to generate the legal agreement (MTA), which streamlines the... with the recipient organization can create an Addgene account and place an order: a purchasing agent,...

...Welcome to Addgene, Aavery!       Original post: In December, we introduced you to Blugene’s new virus...March 6, 2020. After months of input and polling, Blugene's new virus friend finally has a name! We thank ...we’re excited to see your thoughts. Our mascot Blugene can’t wait either. Acknowledgements We would like...

...on the Addgene blog Learn more about Blugene's make-over Get a how-to guide on the new Addgene search... this form or the hashtag #BlugeneVirusFriend on Twitter. As with Blugene’s name, we’d like to give their...receive some newly designed Addgene swag. Have fun! Suggest a name for Blugene's new virus friend here Additional...eight years now has a new friend to join them. Blugene would like you to meet...  Hmmm, they need a name... we need your help in finding a good name for Blugene’s virus friend.  During the month of January,...

.... Along with our role as a plasmid repository, Addgene aims to be a vital educational resource for scientists...mind, we are very excited to debut our brand new Addgene Videos page! Videos are a powerful tool for communicating...or to answer any questions you might have about Addgene. The page is divided into three main sections: ... real Addgenies who have a variety of roles at Addgene.  You’ll hear all about their career paths, future...feature screen-capture footage to help you navigate Addgene’s vast online resources. Our most popular How-To...stage of the selection and checkout process on Addgene’s website: We’ll be continuously updating this...comments section below! Additional Resources on the Addgene Blog 5 Science Rap Videos and Their Creator: Tom...

...their mission. Addgene is fortunate in that we are able to fulfill our mission by generating revenue through... is a bit misleading. Nonprofits can generate revenue (as Addgene does through materials distribution)... at Addgene If you’re ever curious what specifically drives the decision making process at Addgene, just...in the community Addgene fosters a culture of respect, collaboration, and fun Addgene believes there is... on the Addgene Blog Learn about Our Collaboration with Fellow Nonprofit Seeding Labs Addgene Supports...to Our Interview with Addgene Board Member, Connie Cepko Resources on Addgene.org Learn More About Our...Our Mission Meet the Addgene Leadership Team Get the Latest News from Addgene ...

...experiment!” but were unable to find the plasmid at Addgene? Well, we’re here to help with our Suggest a Plasmid...Campaign. How to suggest a plasmid The scientists at Addgene work hard to seek out plasmids to add to our collections... potential depositors. 2. Tweet at us! Tweet @Addgene with #suggestaplasmid, the name of the plasmid ...

...and genetics. It can be used to characterize the function of certain regions in a promoter or gene, as...experiments. When REPLACR-mutagenesis goes head-to-head with Gibson assembly and GeneArt cloning, the results...REPLACR-mutagenesis could save busy scientists a lot of time. Let us know how REPLACR-mutagenesis works ...your hands!     REPLACR-Mutagenesis Megaprimer PCR Gibson Assembly GeneArt Cloning Types of Mutations...Comparison of REPLACR-mutagenesis with other common methods for site directed mutagenesis   References 1. ...al. (2016). “REPLACR-mutagenesis: a one-step method for site-directed mutagenesis by recombineering.” ...Site-directed mutagenesis (SDM) is one of the key tools researchers use to prove causation in molecular...

... Benjie Chen co-founded Addgene in 2004. They worked tirelessly to get Addgene from a concept to an up... culture here at Addgene has remained paramount. Candidates who interview at Addgene frequently ask the...working at Addgene?” Most Addgenies reply with an enthusiastic, “Our company culture! Addgene is the best...ideas and company. The Addgene summer picnic This past weekend was the annual Addgene Summer Picnic - a time... it was another successful event! Addgene company culture At Addgene we organically developed the philosophy... happened naturally when Addgene was a family affair back in 2004. As Addgene has grown, it has taken ...Our story today starts with a bit of Addgene history... As you may be aware, Melina Fan, her brother ...

... be done with RNAi in terms of determining what genes are involved in biological process X, you can do...help guide and promote Addgene in its mission to make research easier. Addgene is excited to announce ...use in genetic screens and has contributed much of his knowledge to the CRISPR resources at Addgene. His... is what got him involved with Addgene - he says he’s “known of Addgene basically since the beginning ...ethic that Addgene has behind it is something that very much resonates with me.” About Addgene’s role in...Doench says, Addgene is “a central resource for what is worth having. The work that [Addgene’s] outreach ...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.       Resources...

...Joanne Kamens Plasmids 101: Antibiotic Resistance Genes By Marcy Patrick Plasmids 101: Yeast Vectors ...community, so email us at blog@addgene.org if you are interested. Addgene's Most Popular Posts of 2014 ...year in science – as seen through the lens of Addgene’s blog and plasmid repository. Our blog is just ... Competent Cells” has been extremely popular, generating many additional questions. We’re happy to see...

...of the optogenetic tools that are still missing. Optogenetic tools for neuroengineering Addgene: For those...brain through optogenetics research Addgene: What are some of the big problems that optogenetic tools can ...science.' Looking for Optogenetics Plasmids? Plasmids for Optogenetics Research (Addgene) Plasmids from Ed...populations of neurons at once. Addgene spoke to Boyden about this latest optogenetics advance, the big questions...brief history of optogenetics? How long has this idea been around? Boyden: Optogenetics is a toolset that...two-color pair. We'll be sending it to Addgene in a matter of days. Addgene: Can you tell us a little more about..., or other conditions. Addgene: You've contributed various plasmids to Addgene over the years, many of...

...promoters, N-terminal tags, coding sequences of the gene of interest, C-terminal tags, plant terminators ...The complete GreenGate toolkit, now available at Addgene, also gives the option to stack several expression...rounds, dramatically reducing the time required to generate multi-construct transgenic plants. In the most...motivations for making GreenGate available through Addgene,” Lohmann said. “We strongly believe the free sharing...Versatile and Efficient Cloning System for Plant Transgenesis." PLoS ONE. 8(12): e83043 (20 December 2013)...

...Caenorhabditis elegans. Genetics, 228(2), iyae126. https://doi.org/10.1093/genetics/iyae126.   Gene disruption in...tags for live imaging in C. elegans CRISPR/Cas9 for gene disruption in M. abscessus  Bioluminescent reporter...developed a novel dual-plasmid CRISPR/Cas9 system for gene disruption in Mycobacterium abscessus, an increasingly...Here, the researchers were able to target multiple genes in M. abscessus either simultaneously or stepwise...system is induced to cause disruption of the target gene. Lastly, curing the mCherry-sgRNA plasmid leaves...Anti-V5 [IPI-SV5-Pk1]. Addgene Report, https://doi.org/10.57733/addgene.l0ulu4. Hanke, T., Szawlowski,...collection of epitope tag antibodies available through Addgene. Anti-V5 [IPI-SV5-Pk1] is a recombinant version...

...sites yourself using PCR, but gene synthesis can save you time. Using gene synthesis, you can request the...If you’re creating a multigene construct, in what position do you want your gene to be in relation to ...to the rest of the genes? And do you want it to be in the same (forward) or in the opposite (reverse) orientation...you a lot of headaches down the line! Cash in on gene synthesis Before you start your cloning, it is advisable... Figure 1: General overview of the MoClo assembly process to produce a multigene construct. Figure...Golden Gate Cloning Additional resources on addgene.org Addgene's MoClo Guide and Kits MoClo Toolkit MoClo...chosen one of the MoClo kits available through Addgene that suits your experimental purposes. But what...

... Sean C., Genevieve Laperriere, and Hugo Germain. "Droplet Digital PCR versus qPCR for gene expression...vectors and improved alternative methods." Human Gene Therapy, Part B: Methods 23.1 (2011): 1-7. PubMed...genomes can limit production of recombinant AAV-2." Gene therapy 6.7 (1999). PubMed PMID: 10455443. 3. Hindson...virus type 2 Reference Standard Material." Human gene therapy 21.10 (2010): 1273-1285. PubMed PMID: 20486768...benchmark for infectivity of recombinant AAV vectors." Gene therapy 17.7 (2010): 872-879. PubMed PMID: 20336156...new viral vector preparations. Addgene has used this method to generally validate our viral vector preparation...Additional Resources on the Addgene Blog Important Considerations When Using AAVs Addgene's AAV Quality Control...

... as ‘indels’. While this is convenient for generating gene knockouts, NHEJ repair does not allow introduction...CRISPR/Cas9 system is a versatile tool for precise gene editing in many organisms and model systems. We ...were able to introduce the desired mutations in genes associated with dementia, such as APP and PSEN1,...Cas-blocking mutations at both targeted alleles when gene editing.   Figure 1: Techniques to improve...called CORRECT, which, by undergoing two rounds of gene-editing enables scarless introduction of an intended...introduction of specific sequence changes. To generate a specific sequence change the cell must undergo...that desired genome-editing events by HDR can be generated more efficiently by (1) improving editing accuracy...

...scarless cloning. The cloning scheme is as follows: the gene of interest is designed with Type IIS sites (such... allows for multiple DNA components (promoters, genes, terminators, etc) to be assembled in the correct...recognition site(s) thus eliminating these from your gene of interest. PCR products are then digested with...ligated following a heat inactivation step. If your genes of interest or destination vector contain multiple...More Resources at Addgene:  Check out more Plasmids 101 Posts! Check out Addgene’s restriction cloning...Addgene’s plasmids are used with a wide variety of restriction enzyme-based cloning methods. Each method...can also be adapted to express multiple gRNAs. Addgene has two Golden Gate-based gRNA assembly methods...

...presence of the protein endonuclease I encoded by the gene endA. Nowadays, the most common E. coli strains ...plasmid amplification are endA negative, meaning the gene has been removed/mutated to neutralize the nuclease...nuclease activity. For strains that still carry this gene, there are usually extra steps like heat inactivation...1874070701307010010 Additional resources on the Addgene blog Find five ways to purify DNA without a kit...Browse our Plasmids 101 blog posts Resources on Addgene.org Check out the Molecular Biology Reference Find...

...scientific domain in general and of our data in particular and our deep understanding of Addgene’s customer’s needs...’t. Try out the new Addgene search engine! Additional resources from the Addgene blog Learn about other...other features on Addgene's website Find a step-by-step guide to using Addgene's new search Read to learn... that we needed to improve more than that: the general “findability” of the information on the site. Different...posts or reagent items.  The technology behind Addgene’s new search  Like millions of other websites, we...approach much more than if we were dealing with general purpose information. Below are a few of the strategies...

...delivery enables minimally-invasive gene delivery and optogenetic excitation. Excitation of neurons deeper...the differences between chemogenetics and optogenetics Resources on Addgene.org Browse all viral vectors...available at Addgene Visit our viral vectors guide Find our chemogenetics and optogenetics science guides...resources on the Addgene blog Read more about the PHP.eB AAV capsid Read more about optogenetics Learn about...Optogenetics is a neuroscience method that lets you fire neurons with the flick of a light switch. Neurons...allowing an influx of ions into the neuron which generates an action potential (Figure 1). This light-induced... to use invasive measures to perform in vivo optogenetic experiments. Intracranial injections of AAV are...

...materials via Addgene. Perhaps if each of many grantors supporting research could provide Addgene with a small...Zuckerberg Initiative for supporting Addgene by providing a generous grant. This support will enable us ...sent the Addgenies home.  At the same time, as Addgene started dealing with this new reality of working...resource for scientists studying the coronavirus. Addgene’s Technology Transfer Team also moved fast to ensure...science sharing as we can get. The effects of Addgene’s brief shut-down and the subsequent fluctuations...don’t have space in this blog to call out every Addgene Team because they’re all doing amazing work during...rose to the challenge was our Finance Team. With Addgene closed for almost two weeks and with labs worldwide...

...in the Addgene repository each year from 2016–2023. B) AAV preps distributed per year by Addgene from 2016...vector preps contain biosensors, optogenetic tools, and chemogenetic tools: protein tools that are activated...available at Addgene from 2016–2023 by type of tool. B) AAV preps distributed by Addgene per year from...biosensor, by Addgene’s categorization) was first developed in 2001, with successive generations developed...As part of Addgene’s 20th anniversary celebration, we’ve been finding and sharing stories unique to our... our repository and resources. As editor of Addgene’s eBooks — including the recently released Viral Vectors...viruses engineered to be safely used to deliver a genetic payload to cells, and the complexity of effectively...

...the newest member on Addgene’s Board of Directors, Bart Newland. Newland is General Counsel at Atalanta... is developing RNAi therapeutics to treat neurodegenerative diseases. He has earned a B.S. in microbiology...like? BN: I've made the transition now to being general counsel of a small start-up. For the last 20 years...and Biogen. They’re very different jobs. Being general counsel at a small start-up is very interesting...interesting. It’s very varied. There’s IP work to do, general corporate work, and negotiating contracts with other...all. Q: How does your experience help you as an Addgene board member? BN: Having seen so much in the business.... I think these are things that I can bring to Addgene -- that comfort with saying, “if we do this, then...

... chances are you are a fan of Addgene or our very cute mascots!  Blugene has been helping us make Plasmids...default backgrounds all year and want a change? Addgene has you covered. Today we’re releasing 11 bold ... purple bacteria video background with mascots Blugene and Aavery? Download the 720p version or download...might want to use one of our virus backgrounds. Addgene’s viral service provides ready-to-use AAV and lentiviral...the joke? If not, you might want to check out Addgene’s Fluorescent Proteins blog topic to learn more ...background.     You can find all of the plasmids that Addgene offers by visiting our collections page. Here, ... green DNA background.     Since we ❤️ DNA at Addgene, we're presenting you with another DNA background...

... first hear about Addgene?  I heard about Addgene from Joanna Brownstein, an Addgene board member. When...resources on the Addgene blog Learn more about about Addgene in our "Inside Addgene" blog series Subscribe...Subscribe to the Addgene blog Resources on addgene.org: Meet the other members of Addgene's Board of Directors...We’re excited to welcome Julie Cicalese to the Addgene Board of Directors. Cicalese served as the Chief...experience and insight to the now 13 member board at Addgene. Let’s meet the newest board member and get to ...School, Joanna was my colleague. She mentioned Addgene was looking for someone with HR expertise to join...serves as an objective body to oversee some of Addgene’s compensation decisions. It was a great time for...

... Figure 1: In conventional gene targeting (A), you must first generate your desired mutation in mouse...mutations, the 8-10 month process of conventional gene targeting is lowered to about 2-3 months. But certain...example of inserting a 2A-FlpO cassette into the Fgf8 gene. Miura et al. (2018) suggest using a 50-80 bp sequence... mouse model generation easi-er! Generating mice before CRISPR The process of generating knock-in mice...process of mouse model generation simpler, as described in an earlier post on generating CRISPR mouse models...after editing. How to use Addgene plasmids for Easi-CRISPR You can use Addgene plasmids to create a repair...template. Figure 2: Overview of ivTRT to generate an ssDNA repair template. Although effective...

...plasmids deposited at Addgene from the abstract page. Check out that beautiful Addgene “Find Plasmids” badge...LinkOut” and search for “Addgene”. Then check the “Link Icon” box next to the “Addgene Nonprofit plasmid repository...Manuscript Resources on Addgene.org Learn How to Order Plasmids from Addgene Check Out Our FAQ Pages ...badge to be taken directly to the plasmids on Addgene’s site (Side note: I always recommend you read the...plasmids badge appear So how do you make this magical Addgene badge appear? First you’ll need to create an account... hope this helps! Additional Resources on the Addgene Blog Get Tips on Keeping Up with the Literature... addgene...

...expand Addgene’s services. There was an overwhelmingly positive response to the option of Addgene providing...allows us to maintain Addgene’s high quality standards?” Under the leadership of Addgene’s co-founder, Melina...In the middle of 2016 Addgene started distributing a small but growing catalog of ready-made AAV and ...Lentiviral preps. This new Viral Service represents Addgene’s largest new initiative since we started distributing... capabilities to produce and distribute virus. Addgene is a 501(c)3 nonprofit and our plasmid service ...build a completely self-supporting service so that Addgene is not dependent on grants. Aiming to operate under...Sponsors and Supporters Page Thank you to all of our generous, sharing depositors. Over 3,000 laboratories are...

... orientation as the gene or use custom gene synthesis or site directed mutagenesis to remove the BbvCI...this. 2) When your gene of interest has a BbvCI site(s). If so, the nicking mutagenesis protocol will still...work if the orientation of the BbvCI site(s) of the gene are in the same orientation as those in the plasmid...Additional Resources on the Addgene Blog Use Site Directed Mutagenesis to Generate Specific Mutations in Your... pot saturation mutagenesis as a quick and easy technique that can be used to generate complex libraries...of this. Plasmid library generation prior to one pot saturation mutagenesis Several methods can be used...effects. One-pot saturation mutagenesis The one-pot saturation mutagenesis technique described in Wrenbeck...

...Versatile Tool for Gene Expression in Mammals Additional Resources on Addgene.org Overview of Viral ...pooled libraries. Whether you’d like to knockout a gene, replace it, modulate its expression, or perform...while rtTA can be used to activate expression of a gene under the control of the tet operator. AAV AAVs ...associated virus (AAV, primarily chemogenetics tools for now but with optogenetic tools coming soon). The viral...excited that Addgene is now offering virion preparations to the scientific community. Addgene's AAV preparations...posts on the blog and pages on the Addgene website. blog.addgene.org The blog provides tips for working...email us and we’ll get you writing!     www.addgene.org On the Addgene website you’ll find our updated viral...