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Showing: 121 - 140 of 390 results

  16. Plasmids 101: Modular Cloning

    Type
    Blog Post
    Published
    March 12, 2024, 1:15 p.m.
    ...References Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S. A modular cloning system for standardized...transcribed together. They can express one or more gene(s), and typically begin with a promoter and end with...

  17. To Each HIS Own

    Type
    Blog Post
    Published
    March 2, 2023, 2:15 p.m.
    ...Natl Acad Sci U S A. 1991;88(20):8972-6. Moks T, Abrahrnsen L, Oesterlof B, Josephson S, Oestling M, Enfors...polyhistidine tag, alternative fusion tags were used, such as S. aureus’ Protein A (Moks 1987). These tags, however...

  19. RNA Extraction Without A Kit

    Type
    Blog Post
    Published
    March 24, 2020, 1:15 p.m.
    ... for at least 4 hours.   If your final RNA sample(s) are resuspended in water or TE buffer, store them...break down RNA upon cell lysis.  In the late 1970’s, Chirgwin and colleagues showed that a strong protein...extraction. Researchers at the NIH in the mid 1980’s set out to develop a protocol that skipped the ultracentrifugation...

  20. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    Published
    Aug. 16, 2018, 12:40 p.m.
    ...compared head-to-head with the E. coli and BioID2 BirA*s in the context of RaPID for their ability to detect...proteins were then subtracted from Ramanathan et al.’s RaPID datasets. Applications of RaPID RaPID sounds..., Rao, D.S., Neela, P.H., Zarnegar, B.J., Mondal, S., Roth, J.G., Gai, H., Kovalski, J.R., Siprashvili...
Showing: 121 - 140 of 390 results