We narrowed to 174 results for: ADAP
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TypeBlog PostPublishedFeb. 20, 2024, 2:45 p.m....by co-incubation of Cas-RNP and assist peptide. Adapted from Zhang et al. 2023 with permission. Find...
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Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene
TypeBlog PostPublishedApril 6, 2021, 12:30 p.m....library. This PCR step also adds the necessary adapter sequences for Illumina sequencing as well as unique... -
Plasmids 101: 5 factors to help you choose the right cloning method
TypeBlog PostPublishedAug. 21, 2018, 12:31 p.m....Gateway cloning protocol is fairly flexible and adaptable to cloning different types of DNA, such as cDNA... -
Plasmids 101: Protein Expression
TypeBlog PostPublishedJune 7, 2018, 1:17 p.m.... grown in well-defined media and can be easily adapted to fermentation allowing for large-scale, stable... -
Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles
TypeBlog PostPublishedJune 22, 2017, 2:30 p.m....colocalization analysis example is given in Figure 2 (adapted from 4) - Dunn et al. 2011 provides an in-depth... -
Truncated gRNAs for Regulating Gene Expression
TypeBlog PostPublishedJan. 10, 2017, 3:37 p.m....interacts with a ≤15nt gRNA that contains an RNA adapter capable of recruiting a transcriptional activator... -
Plasmids 101: Knockout/Knock-In Plasmids
TypeBlog PostPublishedDec. 1, 2016, 3:30 p.m....them by depositing with Addgene! Figures were adapted from: Hall B, A Limaye, & AB Kulkarni. (2009) "... -
Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells
TypeBlog PostPublishedDec. 10, 2020, 1 p.m....through error-free homologous recombination (HR). Adapted from an image provided by Manuel Gonçalves; made... -
Viral Vectors 101: Voltage Indicators
TypeBlog PostPublishedJan. 30, 2024, 2:15 p.m....of the previously described systems. Several adaptations of this method have been to tag, cage, uncage... -
Twenty Years of Addgene Sharing: CRISPR
TypeBlog PostPublishedApril 18, 2024, 1:15 p.m....our requestors. However, we can again see quick adaptation and steady use of all types of libraries, once... -
Plasmids 101: Degron Tags
TypeBlog PostPublishedMarch 14, 2024, 1:15 p.m....to ubiquitination and proteasomal degradation. Adapted from Nabet et al., Chemical Biology, 2018. ... -
Plasmids 101: Optimizing Plasmid Yields
TypeBlog PostPublishedMay 26, 2016, 2:30 p.m....strains are not good for cloning Bacterial strains adapted for protein purification use the cell’s resources... -
Plasmids 101: Multicistronic Vectors
TypeBlog PostPublishedSept. 9, 2014, 8:20 p.m....disadvantages of the IRES element, scientists have adapted "self-cleaving" 2A peptides into their muliticistronic... -
Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection
TypeBlog PostPublishedDec. 12, 2013, 3:35 p.m....medicine. The parts we have right now are well adapted to work in prokaryotic bacteria, not in fungal ... -
SciComm: Taking Science to Elementary and Middle Schools
TypeBlog PostPublishedJuly 9, 2019, 12:52 p.m....formulated the presentation as follows (which could be adapted for any topic--not just CRISPR): Who am I? Where... -
CRISPR 101: RNA Editing with Cas13
TypeBlog PostPublishedJuly 31, 2020, 12:30 p.m...., 2016) as a RNA-targeting CRISPR enzyme, they adapted Cas13b for precise RNA editing (Cox et al., 2017... -
An Introduction to Adenovirus
TypeBlog PostPublishedOct. 3, 2023, 1:15 p.m....cellular events, all recruit strong innate and adaptive immune responses that inactivate viral genomes... -
Getting to Know Expansion Microscopy
TypeBlog PostPublishedJune 20, 2023, 1:15 p.m....model systems, paired with SRM techniques, and adapted for detecting nucleic acids as well as proteins... -
DNA Purification Without a Kit
TypeBlog PostPublishedFeb. 11, 2020, 2:15 p.m....or TE. The Bishop Lab’s glass milk protocol is adapted from the 1979 Patterson Protocol. When published... -
A Guide to Starting Your Own Journal Club
TypeBlog PostPublishedFeb. 6, 2020, 3 p.m....group and topic. For example, it may be useful to adapt the second version (multiple presenter, figure by...