We narrowed to 711 results for: ANG

...positive outcome if the employee sees that real change is possible in your eyes so their confidence is...feedback is a very effective way to bring about change in behaviors and habits in a nonthreatening way... would you do differently? Beware of indirect language You must be honest about performance problems.... I see is managers who hint at or use indirect language to talk about real performance issues. Watch this...this little video to learn more about indirect language from Steven Pinker. As a manager you must be able...There is place in human discourse for indirect language, but it is not the way to go when giving feedback...

..., Wu X, Jiang W, Marraffini LA, Zhang F. Science. 2013 Jan; 339 (6121): 819–23. Mali P, Yang L, Esvelt...other CRISPR subtypes. Still, the large and dynamic range of CRISPR systems between and within prokaryotic...http://en.wikipedia.org/wiki/File:Crispr.png Barrangou R, Horvath P. Science. 315, 2007 Jan; 1709–1712...CRISPRdb. Date: 2013-09-18. Makarova K, Haft DH, Barrangou R, Brouns SJJ, Charpentier E, Horvath P, Moineu...

... The authors share a wealth of data on ... The language is ... The general representation of data is.....couple of sentences - even if written in a clumsy language make it much easier for me to go back and finish...about the paper after my initial read, adjust my language in the first (and clunky) version of my general...general comments and adjust them if my opinion changed after this more detailed look.        Encourage...try to stay away from the loaded and judgemental language. In each peer-review, I try to mention what I ... Tester, where she focuses on (1) salt-induced changes in root-to-shoot ratio in Arabidopsis, (2) expression...

...awarded to study 31 rare diseases,” said Claudia OuYang, BeHEARD Co-Director in a release. The research...developing metastatic melanomas and other cancers. Joshua Wang (bottom right), also of the Wistar Institute, says... broadly. “This [award] is pretty great for us,” Wang said. “We were planning on and looking at a bunch...number of stressors, including emotional stress or changes in temperature.  “The disease was first described...a rare disease that can cause hearing loss and changes in coloring of the hair, skin, and eyes. The researchers...of Waardenburg syndrome (WS4) by Pediatrician Duangrurdee Wattanasirichaigoon carried different mutations...

...they change color as they shift from "off" to "on" in the presence of calcium. This color change can be...imaging neurons, Griesbeck adds that this color change has a particular advantage for observing events...sensitivity. As reported in Nature Methods, the FRET changes of these Twitch sensor variants were optimized ... way to make a FRET sensor with a large dynamic range,” he says. Merkx recently applied this “trick” to...red-shifted FRET sensor for zinc using variants of mOrange2 and mCherry as donor and acceptor domains. He ...

...This post was contributed by guest blogger Eleanor Wang, a Research Specialist in Patrick Hsu's lab at UC...don’t tell us everything there is to know is a strange and exciting revelation. Science in any field can...were inspired by the imminent threat of climate change to study plant and marine biology, environmental...and while doing scientific research. It’s okay to change your mind about what exactly you want to do—I started...can think of to ask! You can DM me on Twitter @EleanorWang. I will do my best to answer or connect you with... help! Thank you to our guest blogger! Eleanor Wang is currently a Research Specialist in Patrick Hsu...other things too. You can follow her on Twitter @EleanorWang.     References and resources References Canning...

...membrane potential in response to visible light (Zhang et al., 2011). What do microbial rhodopsins and ...electrophysiological methods (Deisseroth, 2011; Mei and Zhang, 2012; Towne et al., 2016). Optogenetic neuromodulation...neural activity (Bernstein and Boyden, 2011; Mei and Zhang, 2012). For example, blue light activates channelrhodopsins...Channelrhodopsin-2 to multiple cell types for imaging and long-range circuit mapping." Journal of Neuroscience 28.28 ...237-243. PubMed PMID: 20882. Mei, Yuan, and Feng Zhang. "Molecular tools and approaches for optogenetics.... PubMed PMID: 12665802. SSharma, Suveena, and Jinfang Zhu. "Immunologic applications of conditional gene...PMID: 28772120. PubMed Central PMCID: PMC5830081. Zhang, Feng, et al. "The microbial opsin family of optogenetic...

...meetup.com! Many thanks to our guest blogger Angela Kaczmarczyk! Angela Kaczmarczyk is a co-organizer at BosLab...2015, there were six of us. We were initially strangers but we all shared a passion for DIY Biology and...biohacking courses. Our members pursue a diverse range of projects at BosLab. Some are entrepreneurs using...RNA with small molecules. Other members’ projects range from hacking probiotics to filming educational lab... Angela Abitua...

...followed suit. Two Addgenies, Susanna Bachle and Yijun Zhang, recently attended virtual conferences (TAGC and...and ASGCT, respectively) and Addgenie Angela Abitua hosted a virtual Reproducibility for Everyone workshop...host will see the highly voted questions,” says Zhang. “Usually the moderator starts by asking the speaker... hosted in breakout rooms, or even on social media.Zhang was able to view posters without presenters “...to start. “There was not much time to network,” Zhang says. “The schedule was pretty busy, but after the... Jennifer Tsang...

...bioreporter spontaneously changes from green to orange such that non-growing cells are orange, slow growing cells... But the shape and metabolism of leaves can be changed by light and temperature and leaf autofluorescence...cells are orange-green, and fast growing cells are green. A second colonizer, labelled with a constitutively... Jennifer Tsang...

...B978-012198382-6/50026-1 Wang R, Xiang S, Feng Y, Srinivas S, Zhang Y, Lin M, Wang S (2013) Engineering production...for specificity and sensitivity, for example by changing certain amino acids to improve its stability. ...single protein that can recognize the target protein (Wang et al., 2013). These proteins are also plasmid-derived.../10.1016/j.bios.2016.04.091 Cruse JM, Lewis RE, Wang H, Geziena MT, Steven GE, Lorna J. (2004) ANTIGENS...

... a broad pH range. The four biometric probes successfully targeted endosomes under a range of pH conditions...and RNA expression - but what if you just want to change protein levels? Until recently, no such tool existed...proliferation. These cells also did not contain changes in expression of TRIM21-associated genes, indicating...transcription factors in series and independently changes inducer levels. That is, if transcription factor...expression of a reporter protein, it is possible to change the amount of noise in reporter gene expression...

...trolls. People don’t like to connect with complete strangers as it borders on my first rule of networking--...a useful way to build or use a network. Total strangers can’t really help or refer you. Do not ask people... lot about requesting connections to complete strangers for informational interviews. Many have had success...game for connecting to others.  Pro tip: You can change the option for sending LinkedIn messages to Click...considered intrusive and it’s still connecting with a stranger so is highly discouraged. You should be using ...Turn off notifications to your network of profile changes. If you are working, and all of a sudden you start...

...resolve this issue, Shangqin Guo and her colleagues at Yale University fused a color-changing fluorescent timer...proteins system for Caenorhabditis elegans By Jennifer Tsang Fluorescent proteins (FPs) traditionally introduced...’s native locus, but introducing large genetic changes in C. elegans with CRISPR can be difficult since...synthesis and, after a few hours, a conformational change causes the protein to fluoresce red. H2B-FT-red...j.celrep.2020.107804 The CRISPR corner By Jennifer Tsang Here are a few highlights from recent CRISPR plasmids...

...of [after undergrad] was to learn and speak the language of science. I didn’t come out of [undergrad] feeling...work,” she said. “School will always be there.” Changing your path! It’s okay! Our blog writer, Beth Kenkel...research and technical assistant, when her life changed––she had a daughter. Needing a career switch because...thought, ‘Let me pursue this, let me make a slight change so I can have flexibility and be up to date with...Bioinformatics it is.’” Though she misses the bench, the change gave her the balance she needed. It is incredibly...incredibly helpful to me to know that you can make big changes in your career path and still find comfort and ...

...•T-to-G•C base changes (Figure 1), but it can be challenging to make these base changes, especially in... protospacer, the editing capacity of the ABE8s ranged from 1.5x higher for positions A5-A7 to 3.2x higher...hemoglobin. Previous studies have shown that one base change in the promoter region of gamma hemoglobin (HGB1...effects. ABEs have been described to introduce base changes in cellular DNA and RNA dependent or independent...authors tested ABE8.17-m and found that one base change mutation (ABE8.17-m+V106W) (Gaudelli et al., 2020...

...all incoming plasmids was a big change for Addgene - we had used Sanger sequencing for quality control ...fall within a 10 fold range. Plasmids that don't meet the lower bound of this range are “failed” and removed.... Priority number one was to ensure that this changeover had no impact on our ability to provide requesting...Abudayyeh OO, Platt RJ, Brigham MD, Sanjana NE, Zhang F (2017) Genome-scale CRISPR-Cas9 knockout and transcriptional...

...:10.1126/science.aas9129   Zhang W, Yin J, Zhang-Ding Z, Xin C, Liu M, Wang Y, Ai C, Hu J. In-depth assessment...patient mutation or need to make a precise amino acid change, there is very little flexibility in the location...shown to be less effective at NGH PAMs, though (Zhang et al, & Walton et al). Additionally, SpG Cas9 with...NGAG - xCas9 NG Less effective at NGH PAMs (Zhang et al, 2021) NG Cas9 NG - SpG Cas9  NG  ...

...there were many changes to the  Board of Directors and the Executive Director changed twice. I had to ...liaise with the Executive Director. As members changed, I had to learn how to develop new working relationships... had different styles from their predecessors. Changes were implemented and I had to adapt accordingly...write on behalf of the Advocacy Committee would change format depending on the particular style and requirements...growing too and with that would come necessary changes. I also learnt to adapt my leadership style according...

...O'Neill, P. S., Villette, V., Bradley, J., Wang, Z., Jiang, D., Zhang, G., … Lin, M. Z. (2024). A fast and ... J., Luebbers, A., Green, E., Philibert, C. E., Zhang, H., Layne, M. D., Wu, J. C., & Garcia-Marcos, M...diseases (Diabetes & cancer). Image adapted from Wu and Wang 2024 under a CC-BY 4.0 license. Find more neuroscience... neuroscience antibodies here! Wu, Z., Wang, W. (2024). Anti-APP [6E10-A5] in Immunohistochemistry (Human...