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We narrowed to 158 results for: Arch-GFP

Showing: 121 - 140 of 158 results

  1. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    Published
    Oct. 18, 2018, 12:37 p.m.
    ...mutation can be introduced in a gene, while adding a GFP-tag (Figure 3). Achieving multiple modifications...Molecular Biology. Jake Watson is a postdoctoral researcher in neurophysiology at the MRC Laboratory of Molecular...homologous recombination in vivo." Nucleic acids research 21.15 (1993): 3601. PubMed PMID: 8346047. PubMed...

  2. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    Published
    Oct. 22, 2015, 2:30 p.m.
    ...in this case a reporter gene (e.g. luciferase or GFP), regardless of whether it interacts with a transcriptional...that retain their individual activities (1). Researchers also discovered the modular format of some transcriptional...

  3. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ... a superfolder GFP fusion to stabilize the protein in mammalian cells. LwaCas13a-msfGFP can mediate cleavage...genes that fell into 3 categories based on the architecture of the CRISPR protein in question: C2c1, C2c2...

  4. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    Published
    July 30, 2024, 1:15 p.m.
    ... includes things like viral vectors that deliver GFP.   Figure 3: A) Cumulative AAV preps available...array of ready-to-use viral vectors available to researchers around the world. While we still offer many resources...vectors from the Addgene repository can save researchers significant time and removes a variable from ...2023, we shared 93,491 viral vector preps with researchers in 45 different countries — and as of 2024, we...Addgene catalog would be broadly useful to the research community. It does, however, necessitate us first...the Data Hub before selecting an AAV can help a researcher make a more informed choice on which AAV to request...developed through the GENIE project at HHMI’s Janelia Research Campus on the GENIE website.   Bibliography  ...

  5. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    Published
    Sept. 16, 2020, 1 p.m.
    ...markers were not increased compared to a control AAV-GFP. Not only did the AAV-SaCas9-sgRNA constructs mediate... efficiency than its wild-type counterpart.  In search of a smaller, but equally potent Cas9 protein, ...

  6. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ...aptamers able to bind to a small molecule derivative of GFP called 3,5-difluoro-4-hydroxybenzylidene imidazolinone... instead activates Spinach fluorescence. In the search for something more stable for quantitative imaging...

  7. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ..., Hess et al. successfully evolved wild type GFP to EGFP using CRISPR-X and subsequent FACS sorting. They...with unique tissue-specific promoters expressing GFP, tetracycline response elements, and shRNAs many ... of DsRed connected to a pH-sensitive variant of GFP (SEP) by a 9 amino-acid linker (see Figure 1). The...Plasmids from:  March (Q1) | June (Q2) | September 2016 (Q3) | December 2016 (Q3) MARCH 2016Enhancer activity... much literature searching, DNA synthesis, and plasmid assembly. Luckily, researchers from Douglas Densmore... states. Alexander Stark and colleagues at the Research Institute of Molecular Pathology have developed... library     CRISPR pooled libraries enable researchers to screen the entire genome for genes that regulate...

  8. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    Published
    May 10, 2016, 2:30 p.m.
    ...activated HIV-1, measured via HIV-1 promoter-driven GFP expression. In cell lines that can produce HIV-1 ...latently infected cells. To truly cure HIV-1, researchers need to vanquish these hidden viral reservoirs...“shock and kill” the HIV-infected reservoir, researchers have previously used histone deacetylase (HDAC...could permit some viral protein production, so researchers are also interested in precisely excising the... were used for proof-of-concept studies, but researchers believe that using CRISPR/Cas9 could improve ...represent exciting advances in preclinical HIV-1 research that should be pursued in animal models, and it...benefit many others and accelerate the pace of research. We at Addgene hope to see the problem of in vivo...

  9. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ...are generated by inserting a circularly-permuted GFP into an intracellular loop of various G-protein-coupled...mice under a variety of behaviors, opening up new research directions that were inaccessible with earlier...hope these tools will be useful in your future research, and we’re looking forward to even more innovation...

  10. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    Published
    April 24, 2015, 2:39 p.m.
    ...-2.1 construct can express one of four colors (n-GFP, RFP, YFP or M-CFP.) The construct contains two tandem... and sequence overlap (coral mOrange2, jellyfish EGFP, and sea anemone mKate2.) They then successfully... is retained in Brainbow-3.0, but with mOrange2, EGFP and mKate2 as the fluorophores; mOrange2 is the ...systems. Interested in applying Brainbow to your research? Check out Brainbow plasmids available from Addgene...

  11. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    Published
    Feb. 8, 2018, 12:17 p.m.
    ...fluorescent protein that could be co-imaged with GFP using the same wavelength for excitation. The result...statement: Accelerate research and discovery by improving access to useful research materials and information...post was contributed by Kyle Hooper at Promega. Researchers have been sharing plasmids ever since there were...examples of technologies that can accelerate your research. The creation of NanoLuc® Luciferase from a rather... or above 600 nm wavelengths should be used. Researchers often use bioluminescence to overcome these issues...brightness of NLuc has caught the attention of researchers wanting to perform whole-animal imaging, however...leads to spurious activation of the FRET sensor. Researchers have sought to alleviate many of these challenges...

  12. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ...Church lab uses this system to control expression of GFP.)  To turn this inducible system into a repressible...the complexity of the system, researchers have used split GAL4 architecture or combined the system with ... ON state, and how you might use these in your research. Today, we’ll look at repressible promoters, which...bind the promoter and repress pADH1 activity. Researchers have engineered pADH1 variants that are less ...

  13. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...and labeled with primary antibody (0.01 µg/mL Anti-GFP [N86/38.1R] (Addgene #180084), 0.01 µg/mL Anti-HA...Addgene cloned EGFP into pAG Lenti CMV N-HA Puro (Addgene #236079) to create pAG Lenti CMV HA-EGFP Puro (Addgene...developed by the GENIE Project team at HHMI's Janelia Research Campus (Aggarwal et al., 2025).In this latest ...genetic cargo of choice. After hearing about researchers' favorite features and limitations of existing...excited to share our very own set of empty, HA-, and EGFP-expressing lentiviral backbones designed right here... packaging and strong expression after inserting EGFP and provide step-by-step protocols for viral generation...Figure 2). Looking for more? Explore our empty and EGFP retroviral plasmid set, our bacterial plasmid set...

  14. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ...protein, permeabilized cells  Cy5NTAc 32 25-45 GFP (fluorescein is similar)   Lumio Red Tc: CCPGCC 48... novel aminoacyl tRNA synthetase/tRNA pair, a researcher can lure the ribosome into inserting a fluorophore-tagged...beyond the FRET range of the donor (i.e. >100 Å). Researchers have developed an extensive array of chemical...currently an Assistant Professor in the Anesthesia Research department at Brigham and Women’s Hospital in ... of proteins in living cells." Cell and tissue research 360.1 (2015): 179-194. PubMed PMID: 25743694. ...

  15. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    Published
    March 21, 2024, 1:15 p.m.
    ...labeling. Size is also a factor. Bulkier tags, such as GFP, may interfere with protein expression or function...processes that are crucial in the world of biological research, medical therapy, and even drug development (Grimm...stable, and versatile imaging tools in biological research. This does make finding the right dye for your...complicated. We hope this post helps you in your search for the perfect way to light up your science. Simon...Methods (Vol. 19, Issue 2, pp. 149–158). Nature Research. https://doi.org/10.1038/s41592-021-01338-6 Grimm...

  16. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ...measurable through an epitope tag such as FLAG/His/GFP. Fluorescent protein tags, which correlate closely...and flow cytometry. F1000Research, 13, 817. https://doi.org/10.12688/f1000research.154034.1 Laflamme, ...also includes clone names, which can allow you to search different providers for the same clone. This can...Antibodies community works to make best practices in research antibody selection and use possible, easy, and...quantification of antibody performance in major research applications. eLife, 12, RP91645. https://doi....

  17. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ...cassette comprised of OCT4, SOX2, LIN28, NANOG, and a GFP reporter. Minicircles are removed from cells by dilution...a strategy suited to their research. Find Plasmids for Stem Cell Research Delivery Method Mechanism...layers. Choosing a reprogramming method Most iPSC research falls into two categories: 1) studies focused ...at least one method that can help you with your research goals. Are you a new or experienced reprogrammer...The promise of induced pluripotent stem cells in research and therapy." Nature 481, no. 7381 (2012): 295... Cells Check Out Our Interview with Stem Cell Researcher Darrell Kotton Four Factors that Differentiate...

  18. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...-positive assay) as well as stabilization (Nanog-GFP reporter activation and silencing of factor-linked...along with EGFP while retaining an emission spectrum that is easily separable from that of EGFP. This makes...spectra (N- and C-terminal tags for mTagBFP, TagRFPt, EGFP, mVenus, mCerulean3, mKOFP2) and empty vectors that...and combined it with a variety of reporters (i.e. eGFP) and effectors (i.e. DREADDs). The team demonstrated...undoubtedly constitute a valuable tool for the research community. You can find the AAV vectors for infecting...imaging of two biosensors simultaneously   Researchers in the labs of Michael Lin and Ryohei Yasuda ...mCyFP1, the Lin and Yasuda groups use both mCyFP1 and EGFP as FRET donors in experiments simultaneously measuring...

  19. New Viral Vectors - March 2024

    Type
    Blog Post
    Published
    March 5, 2024, 2:15 p.m.
    ...vector pENN.AAV.EF1a.eGFP.WPRE.rBG AAV PHP.eB Controls Wilson New serotype pAAV-CaMKIIa-EGFP AAV PHP.eB...viral vectors repository this year (and it's only March!)  In our repository, you'll find the latest iteration...Chemogenetics Deisseroth New viral vector pAAV.CMV.PI.EGFP.WPRE.bGH  AAV PHP.eB Controls Wilson New viral ...

  20. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ...fluorescent protein (EGFP) reconstitution. (a) Scheme of validation for DSB mediated EGFP expression cassette...halves of EGFP to recombine by homology directed repair, and resulting in the expression of EGFP. Using ...adaptation for use in mammalian cells in 2012-2013. Researchers are now using this system in ever more creative...Hopefully this post will help get your in vivo research up and running as soon as possible! Selecting ...strand annealing) took place and reconstituted the EGFP expression cassette. (b) pCAG-EGxxFP target plasmid...pCAG-EGxxFP target plasmid contains overlapping 5′ and 3′ EGFP fragments under the ubiquitous CAG promoter. The...can be placed in multi-cloning site (MCS) between EGFP fragments. The pX330 plasmid contains humanized ...
Showing: 121 - 140 of 158 results