...Albert Einstein College of Medicine has recently published techniques to follow translation in living single...presented a novel technology, back then still unpublished, that allowed the visualization of specific de...combination of FUNCAT and PLA PLA was originally published by the Söderberg lab in Sweden as a method for...

...Addgene blogpost and more recently in a paper published in F1000Research on November 2016. Due to the ...were at institutions where no data had been made publicly available (below). However, on November 22nd a...compelled at the state level, in the case of some public institutions) to cancel plans to raise salaries...

...the most similar to yours. Take a look at the publications cited on the manufacturer’s website, which may...highlights a variation of your application, a publication successfully used it in a similar way as you ...safer bet than one that has never been used in a publication. Talk with your peers. If someone in your field...

...are publicly available, including those found at Addgene! Figure 1: Strategy for assembling the...your experiments. Figure 2: Strategy for assembling barcoded combinatorial gRNA libraries. (Adapted...

...purpose. Tang wondered if he could use previously established mouse lines expressing GFP in specific cell types... PMCID: PMC4096992. Find plasmids from this publication at Addgene. 2. Tang, Jonathan C.Y., et al. “...PubMed PMID: 26258682. Find plasmids from this publication at Addgene. 3. Kirchhofer, A., et al. “Modulation...

...contributed by Johnna Roose. This post was originally published on Johnna's New Under The Sun Blog and is part...is science’s way of validating work prior to publication, it doesn’t mean that there are no mistakes or...Manuscripts by Johnna Roose Dos & Don'ts When Publishing a Scientific Manuscript by Maaike Pols, PhD, ...

...for the administrative side of the grant to be established. Take time to read and fully understand the RFA...and develop novel technologies! If data from a publication or preliminary data suggests the feasibility ...will empower the world” tone that differs from publication writing and may be challenging to master at first...

...significant clinical efficacy against a form of blindness known as Leber’s congenital amurosis (LCA). This...RPE65. It is characterized by childhood onset of blindness and was thought to be untreatable until 2008. ...decline in vision with gene therapy in childhood blindness." New England Journal of Medicine 372.20 (2015...

...landscape facing all scientists, both young and established, as they embark on or continue their careers ...Advisor MIT Washington Office Ben Corb - Director of Public Affairs at ASBMB Adam Fagen, PhD - Director, Early...science and in the intersection between science and public policy. Follow David on Twitter @driglar.   References...

...PubMed PMID: 27272384 Find plasmids from this publication at Addgene. 2. Kleinstiver, Benjamin P., et ...PubMed PMID: 27347757. Find plasmids from this publication at Addgene. 3. Zetsche B, Gootenberg JS, Abudayyeh...PubMed PMID: 26422227 Find plasmids from this publication at Addgene. 4. Makarova KS, Wolf YI, Alkhnbashi...

... in situ hybridization experiments. A recent publication [1] from the Sánchez lab describes the plasmids...the deposit) are described in the Sánchez lab publication [1] so that the students performing these experiments..., is available from Addgene. If you use these published protocols and want to share pictures, feedback...

...Originally published Aug 3, 2017 and last updated Apr 6, 2021 by Will Arnold. Introducing NGS in 2017... that contain synthesized regions that have no publicly available reference sequence or plasmids containing...these plasmids are returned as one or two partial assemblies. If we obtain an NGS result that is useful, but...

...instances. If your poster material is nearing publication, consider uploading your work to a preprint server... this is being used, it’s best to turn to an established social media platform. During the conference ...something on social media. If someone is presenting unpublished data they may ask the audience to not share their...

...do this internally, but they are not available publicly. Furthermore, we did not find any tools which ... is generally advised, there is little detail published on how to do it best. Scientists are typically...the How To Guide. Conclusion CrispyCrunch is now publicly-available for high-throughput design and analysis...

... for making targeted modifications. A recent publication by Zhang et al. shows just that - using MMEJ ...PMID: 26678082. Find the plasmids from this publication at Addgene. 2. Dalvai, Mathieu, et al. (2015...633. PMID: 26456817. Find plasmids from this publication at Addgene. 3. Zhang, Chi, Xiuhua Meng, Xiaolei...

...techniques. More and more CRISPR tools are being published each month, making CRISPR a great choice for your...to consult the literature to see if anyone has published a protocol that would work for your system. Addgene... are a few plasmids at Addgene that have been published as CRISPR testing tools: Traffic Light Reporter...

...emerging work by collaboratively discussing recent publications in a specific field(s). Most anyone who has ...stay up to date and engaged with new advances in published science. They also serve as a way to learn about...blog posts centered around scientific papers and publishing Find tips for leading a great meeting ...

...This post was originally published on the Quilt Genomics Blog and is republished here with permission. Quilt...identified 23-mer gRNA. Off-target determination was established with Bowtie2 using the parameters first described...

...early I’m fortunate to live in a town with a good public school system that has made strong efforts in the...them had imagined for a scientist. I think that establishing this history helped demonstrate that a) I was...examples I used in my presentation had only become public in the previous two weeks, which demonstrated to...

...protein instead localizes to the cytoplasm? Or more troubling, what if a significant fraction of the fusion ...properly? We suggest starting by using the newly published oxidative optimized palette of FPs (moxFPs) [5... non-optimized parental proteins. Our recent publication illustrated the quantitative increase in fluorescent...