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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 271 results for: CAD

Showing: 121 - 140 of 271 results

  1. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    Published
    June 3, 2021, 1:15 p.m.
    ...variables (scFvs)? scFvs weren’t developed for another decade when in 1988 they were cloned by two separate labs... Escherichia coli. Proceedings of the National Academy of Sciences 85:5879–5883 . https://doi.org/10.1073...

  2. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    Published
    Oct. 11, 2016, 2:30 p.m.
    ...genome engineering tools that are available to academic researchers through Addgene. CRISPR components...coordinate gene silencing in mature plants. Proc Natl Acad Sci USA 116:7543–7548 . https://doi.org/10.1073/...

  3. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    Published
    Aug. 23, 2016, 2:30 p.m.
    ...The focus of this article will be talks for non-academic settings. Many of these guidelines will apply ...presentation, but, for more detail on the job talk for an academic position, see this very good article at NatureJobs...

  5. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ... by tetracycline-responsive promoters. Proc Natl Acad Sci U S A. 1992 Jun 15;89(12):5547-51. doi: 10.1073...derived from the trp and lac promoters.” Proc Natl Acad Sci U S A. 80(1) (1983): 21-5. PubMed PMID: 6337371...

  6. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    Published
    March 8, 2021, 2:41 p.m.
    ...antigen structure. Proceedings of the National Academy of Sciences 76:3116–3120 . https://doi.org/10.1073...some applications. Proceedings of the National Academy of Sciences 76:4350–4354 . https://doi.org/10.1073...

  7. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    Published
    July 12, 2016, 2:30 p.m.
    ...the Dorr Mountain with the rest of the skyline of Acadia National Park. I was confused. It was supposed ...professor at the Institute of Zoology, Chinese Academy of Sciences, and he is interested in using CRISPR...

  8. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    Published
    May 31, 2016, 3:30 p.m.
    ...transposase tools for genome engineering.,” Proc. Natl. Acad. Sci. U. S. A., vol. 110, no. 25, pp. E2279–87, ...tool for conditional cell ablation.,” Proc. Natl. Acad. Sci. U. S. A., vol. 105, no. 38, pp. 14492–6, Sep...

  10. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    .... PubMed PMID: 25895059 Barnea, et al. Proc Natl Acad Sci U S A. 2008. PubMed PMID: 18165312 CIDAR MoClo...PubMed PMID: 24136966 Nyerges, et al. Proc Natl Acad Sci USA. 2016. PubMed PMID: 26884157 Aronshtam...protein chains or clusters. Veggiani, et al. Proc Natl Acad Sci U S A. 2016. PubMed PMID: 26787909 EasyClone...

  12. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ...recommendations for improving antibody use and reliability (Roncador, et al., 2015; Taussig, et al., 2018; Uhlen, et...Nature 518:27–9. https://doi.org/10.1038/518027a  Roncador G, Engel P, Maestre L, Anderson AP, Cordell JL...

  13. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    Published
    June 8, 2021, 1:15 p.m.
    ...,” but taking the first step into the world of academic research can be quite daunting. During my first... are also not limited to working at a bench in academia or industry, so feel free to explore! Addgene ...

  15. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    Published
    Jan. 19, 2021, 2:15 p.m.
    ...been infected with a microbe, to trigger immune cascades that clear these infections. Anything that generates...immune response and initiates different immune cascades. In the research setting, antibodies of different...

  16. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    Published
    Oct. 24, 2017, 1:54 p.m.
    ... I have spent the last 15 years training as an academic scientist. I’ve worked tirelessly to get the publications... our research to family and friends outside of academia. But the fact is that we are citizens first and...

  17. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ...tRNA-processing system.” Proceedings of the National Academy of Sciences USA 112(11) (2015): 3570-5. PubMed ...engineering in Drosophila.” Proceedings of the National Academy of Sciences USA 111(29) (2014): E2967-2976. PubMed...

  18. CRISPR Guide Update

    Type
    Blog Post
    Published
    Oct. 10, 2024, 1:15 p.m.
    ...make large genome edits using transposases or the Cascade-Cas3 system. We also describe newly engineered ...

  19. New FLIS Plasmid (April Fools 2022)

    Type
    Blog Post
    Published
    April 1, 2022, 1:15 p.m.
    ... research, particularly genetics research, for decades. Its short life cycle, reasonably close genetic...
Showing: 121 - 140 of 271 results