...antibody production is Komagataella phaffii (a.k.a  Pichia pastoris) because it secretes fewer of its own ...include Human Embryonic Kidney (HEK) cells and Chinese Hamster Ovary (CHO) cells. (L'Abbé et al., 2018...-8730-6_1. PMID: 30242676. Frenzel A, Hust M, Schirrmann T. Expression of recombinant antibodies. Front....org/10.1016/j.cocis.2014.10.002. Randolph TW, Schiltz E, Sederstrom D, Steinmann D, Mozziconacci O, Schöneich...

...the term ‘grant writer’, but make no mistake, researching, winning, and effectively managing grants involves...development, certification, degrees, foundations, coaching and even Twitter Chats dedicated to the Grant ..., e.g. education, poverty, environment, early childhood development, or community development? Do you ...consultants who offer grant training, mentoring and coaching services, including Smartegrants, to help those...

...transfer vectors based on whether or not they have a chimeric 5'LTR. Second-generation transfer plasmids have... while 3rd-generation transfer plasmids have a chimeric 5'LTR that includes a CMV or RSV promoter as well... well as a portion of the 5'LTR. Including a chimeric 5'LTR removes the requirement for the HIV Tat protein...SIN is shorthand for self-inactivating, which is achieved by deleting a large portion of the 3'LTR in the...

... cell lines that do not express these sugars. (Takeuchi et al., 1997). The alphaGal sugars end up in the...withstand ultracentrifugation which helps scientists achieve a high titer (reviewed in Joglekar and Sandoval... can bind and transduce many cell types before reaching the target cells. VSV-G is also toxic at high ...832 . https://doi.org/10.1182/blood-2001-11-0042 Takeuchi Y, Liong SH, Bieniasz PD, Jäger U, Porter CD, ...

...create unmarked genetic modifications in the yeast Pichia pastoris. By combining both positive and counter...first described by studies of EcoRI and Bsp6I in Escherichia coli in 1995 when introduction of restriction-modification..., Hanna Engelberg-Kulka, and Gad Glaser. "An Escherichia coli chromosomal" addiction module" regulated...counter-selectable marker for unmarked genetic modification of Pichia pastoris." FEMS yeast research 9.4 (2009): 600-...

...recombinase have been developed. A common means to achieve ligand control is to fuse a mutant estrogen receptor...be transfected by in utero electroporation. To achieve sparse labeling of individual neurons one could...Central PMCID: PMC41617. 7. Metzger, D, J Clifford, H Chiba, and P Chambon. 1995. “Conditional Site-Specific...Recombination in Mammalian Cells Using a Ligand-Dependent Chimeric Cre Recombinase.” Proc Natl Acad Sci U S A 92(...

...genes that fell into 3 categories based on the architecture of the CRISPR protein in question: C2c1, C2c2...single crRNA The Feng Zhang lab used Cas13a from Leptotrichia shahii (LshCas13a) to begin to characterize ...specific RNA sequences from a population (either enriching or depleting specific RNAs out of a pool of RNAs...et al., 2017). They characterized Cas13a from Leptotrichia wadei (LwaCas13a), using a superfolder GFP fusion...

... generally two to three fold higher than those achieved using the standard qPCR method.  Titering by the...preparations is assayed by comparing the relative stoichiometric ratios of the viral capsid proteins VP1, VP2...transfer plasmid Addgene uses a rigorous barcode matching system that follows the sample from the DNA tube...    Figure 4: Schematic of the barcode matching system and PCR-based confirmation of transfer ...

...plasmids rely on their host cell's replication machinery in order to replicate—but not always to the same..., as they only require the host's replication machinery for elongation and termination. These types of... folding and blocks RepZ translation (Asano & Mizobuchi, 1998). This system keeps RepZ levels low even...doi.org/10.5772/19313 References Asano, K., & Mizobuchi, K. (1998). Copy number control of IncIalpha plasmid...

...general, or anxiety associated with psychiatric diseases such as schizophrenia or bipolar disorder. In both ...found that downregulating expression of miR124a achieved a similar improvement in the forced swim test....the BDNF hypothesis of depression?." Molecular psychiatry 12.12 (2007): 1079. PubMed PMID: 17700574. Hare...

... are part of engineered chimeric antigen receptors (CARs). CARs are a chimera of scFvs and T cell receptors...anti-digoxin single-chain Fv analogue produced in Escherichia coli. Proceedings of the National Academy of ...A, Sivaccumar JP, Ruvo M (2020) Evolution of Escherichia coli Expression System in Producing Antibody ...

...1: Indirect detection of target markers can be achieved through two-protein labeling by utilizing(a) biotin-streptavidin...e.g., for cellular activation. In addition, FACS achieves greater purity of your sorted cell sample than...proliferation between cell populations can be visualized, achieved through, for example, a previously induced gene... your marker of interest. Indirect labeling is achieved through protein-tag or protein-protein interactions...

...   Many thanks to our guest blogger, Joachim Goedhart! Joachim Goedhart is an assistant professor at ...This post was contributed by guest blogger Joachim Goedart, an assistant professor at the Section of ...fluorescent probes. You can follow him on twitter: @joachimgoedhart.   References 1. Zacharias, David A., et al...

...pseudopregnant female mice. If you obtained the desired chimeric mice, you would breed them many times, hoping ...line with a recombinase. If it turned out your chimeric mice did not contain edited germ cells, your experiment...inject the ES cells into a mouse embryo to create chimeric mice. This laborious process takes ~8-10 months...When delivering the ssDNA template and CRISPR machinery, Quadros et al. recommend ribonucleoprotein (RNP...

...have created many engineered serotypes by mixing, matching, and modifying capsid and genomic structures. ...., Brown, K. E., Walters, R. W., Zabner, J., & Chiorini, J. A. (2001). Adeno-Associated Virus Serotype...Kuleshova, E., Ojala, D., Huang, C.-C., Gerfen, C. R., Schiller, J., Dudman, J. T., Hantman, A. W., Looger, L....M., Keshavjee, S., Brown, K. E., Welsh, M. J., Chiorini, J. A., & Zabner, J. (2001). Binding of adeno-...

...engineered from the cmt and cym bacterial operons. A chimeric transactivator, cTA, was created by fusing CymR...the system, researchers have used split GAL4 architecture or combined the system with Flp/FRT, as reviewed...) to GAL4 or VP16 activation domain results in chimeric GAL80-repressible and GAL80-insensitive activators...lethality in flies. Riabinina et al. also developed chimeric transactivators GALQF and LexAQF, which activate...

...In an era where millions of people hold a PhD, achieving our career goals can be a real challenge. On top... hinder them from advancing their careers and achieving success.” Pedro Gonzalez Menendez (Mentee IMP ...compared to their pre-program self-assesment. Achievements directly attributable to participation in the...interested in breast cancer and metastasis as well as teaching and mentoring activities. Follow her on twitter...

...optimal/minimal injection titer and volume needed to achieve adequate expression in your cells of interest.  ...waiting periods and/or higher injection titers to achieve adequate expression. Conducting a time course experiment...to minimize off-target expression while still achieving adequate expression in your cells of interest....different brain region that the same result will be achieved. The fact is that there are many known and unknown...

...2. Fluorescence Activated Cell Sorter (FACs) A machine that sorts cells according to whether or not they... deflect them into appropriate containers. The machine is useful for distinguishing cell populations that...21596592. PubMed Central PMCID: PMC3164316. 5. Chieppa, Marcello, et al. "Dynamic imaging of dendritic...165. PubMed PMID: 18193049.  9. Filipe-Santos, Orchidée, et al. "A dynamic map of antigen recognition ...

...2017 contest winners:   Chicagoland's Community Biology Lab (@ChiTownBio) and one of our favorite twitter...holiday cheer, and we loved it! Check out their matching "MACK ATTACK!" holiday sweaters. Alex Dainis ...