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We narrowed to 383 results for: FLU

Showing: 121 - 140 of 383 results

  1. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    Published
    Nov. 19, 2013, 2:37 p.m.
    ...direct fusion of fluorescent proteins (FPs) to a yeast gene of interest. These fluorescently tagged genes ...several ready-to-use, modular plasmids, combining fluorescent tags, epitope tags, protease sites, and selection...of course—and start tagging! Yeast-optimized fluorophores for imaging Lee S, et al. (2013) PLoS ...marker. Lee et al.(1) assessed many of these fluorescent tags in S. cerevisiae, looking at their performance...requirements. Select from these yeast-optimized fluorophore tagging vectors for your single or multi-color...

  2. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

    Type
    Blog Post
    Published
    June 19, 2018, 1:38 p.m.
    ... vivo imaging with fluorescent proteins Browse luciferase plasmids Find fluorescent protein biosensors...developed in vivo bacterial reporters that use fluorophores and luciferases to track bacteria in real time... reporters Limited Depth of Signal/Imaging: Fluorescent reporters are not as well suited for imaging ...context/spatial localization: In vivo imaging with fluorescent or bioluminescent reporters usually only detects...tools The luciferase reporter gene The green fluorescent protein reporter gene Resources on Addgene.org...

  3. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    Published
    Dec. 14, 2020, 2:15 p.m.
    ...FRET), in which a donor fluorophore is used to excite an acceptor fluorophore. The difference for BRET...blog Browse all of our fluorescent protein blog posts Find other fluorescent biosensors Resources on...transfer is between a luminescent donor and a fluorescent acceptor. TRUPATH uses an updated version of ...on Addgene.org Browse the catalog of fluorescent protein plasmids Find biosensors for your experiments...

  4. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    Published
    April 10, 2018, 12:56 p.m.
    ...and instead are conjugated to reporters (i.e. fluorescent dyes, HRP, etc. ) after purification. Antibodies...signal amplification occurs when HRP converts a fluorescently labeled tyramide molecule into a highly reactive...linked to residues within close proximity thus fluorescently labeling them. HRP RANbody staining results ... ≥100-fold increase in signal over intrinsic fluorescence. Increased signal is likely due to lower background...and tissue sections Amplifying weak signals of fluorescent protein (i.e. GFP, RFP) Detecting GFP reconstitution... from Penn State Need help picking the right fluorescence microscopy technique to use? Check out this ...

  5. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    Published
    Jan. 5, 2021, 2:15 p.m.
    ... in bacteria CRISPR-Casɸ mGold, a new yellow fluorescent protein New CRISPR plasmids New items from the...32675376/ mGold, the most photostable yellow fluorescent protein to date By Justin Ng High-throughput ...High-throughput screening methods, such as fluorescence-activated cell sorting (FACS) helps researchers sift through...million cell types expressing variants of Yellow Fluorescent Protein (YFP). The team identified a new YFP ...Resources page.  CDDR (CRISPR–Cas9-based Dual-fluorescent DSB Repair) is a double-strand break repair assay...the introduction & resolution of two DSBs in a fluorescent reporter. Find the plasmids here Yongsub Kim...

  6. Hot Plasmids: Summer 2025

    Type
    Blog Post
    Published
    Aug. 28, 2025, 1:15 p.m.
    ...in this post: Bright and photostable yellow fluorescent protein mGold2 Viral delivery of TnpB for plant...connections with ATLAS Bright and photostable yellow fluorescent protein mGold2 By Mike Lacy Get your sunglasses...these proteins are bright! Most current yellow fluorescent proteins tend to photobleach rapidly, which hinders...St-Pierre recently developed a pair of new yellow fluorescent proteins: mGold2s and mGold2t (Lee & Lai, et ...using mVenus or mCitrine, or if existing yellow fluorescent proteins just haven’t met your needs, consider...et al. (2025). Bright and photostable yellow fluorescent proteins for extended imaging. Nature Communications...

  7. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ...quenched fluorescent ssRNA reporter is added to the reaction. Cleavage of the quenchable fluorescent RNA by...Cas13 and Cas12a, are combined with different fluorescent reporters for each enzyme. In addition, Csm6,...Csm6 and a Csm6 specific reporter (in the same fluorescent channel as the Cas13 reporter), they could significantly...papilloma virus (HPV) genome, via a crRNA. An ssDNA-fluorescently quenched (FQ) reporter, which will produce a...rate of ~1,250 cuts per second. A quantifiable fluorescent signal designates the presence of your DNA of...

  8. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ... highly autofluorescent tissues like the intestine. Figure 3: Expression of fluorophore-tagged ... imaging in C. elegans By Alyssa Neuhaus Red fluorescent proteins like mScarlet are essential tools for...2024 under CC-BY license.  These optimized fluorescent proteins will be a useful addition to the toolkit...Comparative analysis of new mScarlet-based red fluorescent tags in Caenorhabditis elegans. Genetics, 228...wavelength (460 nm) is shorter than many popular fluorescence markers like GFP (510 nm), allowing co-imaging...

  9. Editor's Choice, July 2016

    Type
    Blog Post
    Published
    Aug. 5, 2016, 3 p.m.
    ...biological phenomena and about how fluorophores other than fluorescent proteins can be incredibly useful...CRISPR variant going forward.   Special Delivery Fluorophore Targeting for FRET Studies While I love all of...This post describes how to target and use FRET flurophores for a variety of applications. While editing ...Comparison to Cas9 and NgAgo Special Delivery: Fluorophore Targeting for FRET Studies Gendered Innovations...

  10. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    Published
    Oct. 8, 2020, 1:15 p.m.
    ...you can use PCR based detection, DNA staining, fluorescent tagging, agar plating, etc. Be wise to choose... to detect mycoplasma. When observed under a fluorescent microscope, the mycoplasma DNA appears in the...small clusters, apart from the cellular DNA. Fluorescent DNA staining is another DNA staining alternative...contamination by other bacteria or extracellular fluorescent signals produced by these bacteria can hinder...culture media. Also, drugs like BM Cyclin, fluoroquinolone ciprofloxacin, ciprobay, zagam, baytril, tetracycline...Method for the Detection of Mycoplasmas Using Fluorescence Microscopy. Cells 8:1510 . https://doi.org/10.3390...

  11. Antibodies 101: Epitope Tags

    Type
    Blog Post
    Published
    May 11, 2023, 1:15 p.m.
    ...used for western blot, flow cytometry, and immunofluorescence . However, protein purification is not ideal...amino acid peptide (YPYDVPDYA) derived from human influenza hemagglutinin. The tag has two negatively charged...for immunoprecipitation, western blot, and immunofluorescence with some success for protein purification...and can serve as an epitope tag as well as a fluorescent marker. The GFP tag is ideal for live cell imaging...imaging, FRET, flow cytometry, or immunofluorescence where an antibody can’t be used or that step can ...

  12. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    Published
    Aug. 2, 2018, 12:56 p.m.
    ...separate DNA or transforming cells with green fluorescent protein, these activities are limited to those...Typically, if you want to make a protein – say green fluorescent protein – you first need the DNA sequence that... instructions it needs to produce that green fluorescent protein through transcription and translation...students can easily sense, whether that’s bright fluorescent proteins that they can see, enzymes that can ...Stark, Jessica C., et al. "BioBits™ Bright: A fluorescent synthetic biology education kit." Science Advances...

  13. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    Published
    Nov. 27, 2018, 2 p.m.
    ...various structured surfaces. Plasmids expressing fluorescent proteins will be used to tag individual strains...within the biofilms will then be monitored using fluorescence microscopy and FACS. In an additional approach...this condition is crucial. Plasmids expressing fluorescent proteins and a new two-photon laser scanning ...plasmids containing promoter regions that activate fluorescent protein transcription in response to river contaminants...water samples at specific locations, measure fluorescent signals from the sensor, and send a signal including...

  14. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    Published
    Jan. 31, 2019, 1:32 p.m.
    ...microenvironment enzymes and metabolic reprograming influence tumors development? Being pathological active ...to cure cancer. To understand how cancer cells influence microenvironments and vice versa, let’s take a... being shaped by them. Another set of highly influential microenvironment cells are Cancer-Associated ...fact, Sanford H. Barsky and his team tested the influence myoepithelial cells had on the conversion of non-malignant...begun to understand two main areas of study that influence tumor microenvironments: immunology and angiogenesis...

  15. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...colleagues identified a highly bright and stable fluorescent protein, StayGold. Although it quickly saw wide...dimerization while maintaining the bright and stable fluorescence of the original (Ivorra-Molla et al., 2023)....Ivorra-Molla, E., et al. (2023). A monomeric StayGold fluorescent protein. Nature Biotechnology, 1–4. https://doi.org..., et al. (2023). Bright and stable monomeric fluorescent proteins derived from StayGold. ResearchSquare...mature marmosets. CAP-Mac also robustly expressed fluorescent reporters in green monkeys and cultured human...

  16. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...implications for the fusion protein effectivity), and a fluorescent protein tag for visualization (Fig. 1b). While...catalytically inactive dCas9) to modify chromatin. A fluorescent protein can still be included for visualization....   G-baToN can also be applied to other fluorophore/antibody pairs. Because the anti-GFP nanobody...quantify protein degradation the Ward lab used fluorescent proteins (FPs) fused to the AID* (minimal 44 ...Saccharomyces cerevisiae. To control Cas9 activity and influence DNA repair pathways, Amit Choudhary's lab developed...

  17. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    Published
    Sept. 3, 2020, 12:15 p.m.
    ... FAST system, Ye’s lab could edit a tdTomato fluorescent reporter gene in mice using Minicircle DNA vectors...of Cas9 and a photo-dissociable dimeric green fluorescent protein (pdDronpa) (Zhou et al., 2018). pdDronpa... flanking the DNA-binding cleft of Cas9, the fluorescent domains dimerize and prevent DNA binding in the...chromatin architecture (Hoffman et al., 2020). Fluorescent dCas9 proteins can be used to identify specific...gRNA and a dCas9 fusion protein with three red fluorescent tags in U2OS cells. Upon light activation, dCas9...figure below. Next, by using a dual reporter fluorescence assay, Hemphill et al. demonstrated that the...

  18. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    ...Which Fluorescent Protein Should I Use? Plasmids 101: Green Fluorescent Protein (GFP) Fluorescent Protein...Plasmids 101 has covered GFP and its related fluorescent proteins, which are sometimes used as tags for...tag for purification? Or you want to combine a fluorophore with a tag that localizes your protein to the...

  19. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ...better ability to record subthreshold voltage fluctuations, while having high fidelity during action potentials...Structure of Voltron or Voltron2 conjugated to a JaneliaFluor dye emitting at 525 nm. Image reused from https...Because they are negative-going sensors (with fluorescent signal at baseline voltage), the investigators...closely-packed labeled cells. Plus, Voltron2’s fluorescence is tunable by changing the dye that becomes ...6).   Figure 6: Illustration of split fluorophore assay to test reconstitution via mRNA trans-splicing...

  20. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    Published
    June 3, 2021, 1:15 p.m.
    ...The SunTag system uses an scFv to amplify the fluorescence intensity of a tagged protein. SunTag has two...bind the SunTag scaffold, the intensity of the fluorescent signal is boosted and enables single molecule...contains an HA-binding scFv that’s fused to a fluorescent protein such as GFP. The HA frankenbody works...frankenbodies, an anti-HA scFv-based probe for fluorescent imaging. Learn more about antibodies  Read about...SunTag system which uses an scFv to amplify the fluorescence intensity of a tagged protein. Resources on...
Showing: 121 - 140 of 383 results