...decades, recombinant Adeno-associated virus (AAV) has been widely used to deliver genetic tools to the ...The most famous ones are influenza hemagglutinin (HA), myelocytomatosis viral oncogene (myc), simian virus...limitations of epitope tags and fluorescent proteins, it has been crucial to develop new tracers that enable accurate...are known to be notoriously difficult to label. It has also been shown that dendrites in the stratum oriens... provided better labels than fusions to 3 or more HA tags. 4. High-resolutions microscopy As indicated...

...Addgene's Special Collections). Arguably, oncology has benefited most as cancer cells are amenable to culture...misidentified cell lines. The solution Fortunately it has never been easier to conduct high quality cell culture...identity. For example, perhaps a working cell bank has been made by a research institution (an excellent...at the scene of a crime (every human in the world has a unique STR profile) and can also be used as a paternity...topics. The LGC Group also provides standards and has laboratories to carry our independent quality control...

...firm The path to a career as a Grant Professional has been a long and winding path for many in the field...?  In the past 5-10 years, the Grants Profession has become just that, a profession. A grant professional...and the increasing regulatory process for grants has fostered the demand for experts in the field of grants... find your people Find someone who is working or has worked as a Grant Professional to mentor and coach...includes the social media community GrantChat. She has served in the nonprofit and government sector for...

... in situ. Used since the mid-90s, this technique has revolutionised the way we apprehend molecular complexes...protein interactions in living cells. Since then FRET has also been used to measure conformational changes,...brightness - even though in the most popular pair, CFP has five-fold less brightness than YFP. The stoichiometry...Fluorescence lifetime imaging microscopy method FLIM has been developed more recently and is the most rigorous...emergence of faster measurement systems, this technique has still many good days and good “gigs” ahead...No offense...

...policy. Since early March, the open access publisher has required authors to include a note as to where readers...how much data was necessary and reasonable. PLOS has since clarified their data sharing policy and recently...Who is to say that the initial data-generating lab has efficiently trawled their own data? A distinct (perhaps...many have multiple, complex panels. PLOS, itself, has not finalized its definition of necessary data requirements.... Secondly, science is highly technical and this has a natural, albeit unintentional, tendency to work...

... and polling, Blugene's new virus friend finally has a name! We thank you for your participation in suggesting...play on viral vector terms. And, the name Kendall has a special place in our hearts: our first two offices...

...Training as a scientist in the academic system has many pluses. I delighted in my graduate school years...call this building relationships because networking has gotten a bad reputation (as in…”I just hate networking...connections. A mistake made by many novice networkers has a potential to alienate connections. If meeting with...

...ElizabethKenkel) December 5, 2018 How social media has helped me in science It may have taken a bit of FOMO...Here are three examples of how using social media has helped me or my lab mates: For me, using social ...with social media as a scientist Using social media has enriched my professional development, and while it...helped you fit social media into your busy schedule. Has social media helped you advance your research or ...

...being targeted via RNAi. Recently the Vellai lab has created an improved RNAi vector that leads to significantly...sequence of the toehold switch. For example, Green has also developed toehold switches to detect Zika virus...protein motifs than previously thought. Sidhu’s group has made several hundred SH3 domain containing plasmids.... In addition to these constructs, the Sidhu lab has made hundreds of additional plasmids available through...

...targeting. Fast forward a decade, and the CRISPR field has exploded with dozens of Cas enzymes and variants ...scientific community. What are the features? CasPEDIA has collected relevant information on all the Cas enzymes...cleaves both strands of its dsDNA target in cis and has no trans activity. AsCas12a, on the other hand, generates...learn about Cas domains. CasPEDIA either directly has this information or links out to this content and...

...and post-docs alike are wondering where the time has gone. Are we any closer to graduating, publishing...unsure of the next steps. Harvard PI Angela DePace has put personal development at the center of her lab... and postdoc training. On the other side, the PI has an opportunity to check in with lab members, determine...

... field of induced pluripotent stem cells (iPSCs) has been around for 10 years. In that time, scientists... of Myc or other oncogenes after differentiation has been linked to tumor formation in iPSC-derived and...iPSCs compared to other delivery methods. Retrovirus has a cloning capacity of ~8 kb, so either a single polycistronic...factors driven by one promoter. While lentivirus has similar rates of reprogramming when compared to MMLV-derived...mechanism between a vector and chromosomal DNA. PB has a cloning capacity of ~9-14 kb. This system is transfected...cell division. A drawback to this approach is it has lower levels of efficiency at generating iPSCs, usually...reprogram cells is that it’s simple and efficient. It has a high efficiency of reprogramming when compared ...

...to find the perfect combination. The EcoFlex Kit has a wide array of DNA parts that can be assembled into...yeast MoClo is also for eukaryotes! The technology has been used to optimize protein secretion and expression...possible through MoClo. The MoClo Plant Parts Kit has everything you would expect in a MoClo kit plus the... general plant cloning experiments. Addgene also has two additional MoClo plant kits: Plant Parts Kit ...; Chamness et al., 2023). The Plant Parts II kit has a focus on infrastructure with shuttle vectors, several... gene silencing vectors. The Plant Parts III kit has a focus on transformation and genome engineering....Brychcy et al., 2023). For plant biologists, Addgene has not one, not two, but three CRISPR MoClo kits for...

... 16, 2020 by Beth Kenkel.  CRISPR genome editing has quickly become a popular system for in vitro and ...packaging capacity of AAV (~4.7 kb). While this approach has been proven feasible, it leaves little room for additional...the number of targetable loci, and truncated Cas9 has much lower efficiency than its wild-type counterpart...amino acids, can also be packaged into AAV. NmeCas9 has the added advantage that it can be turned off by ...daunting, it’s actually a challenge that the field has overcome with split AAVs. In general split AAVs break...

... at the Georgetown University School of Medicine has used this gene editing system frequently over the...in human cells (Kim et al, 2016). The Waldman lab has designed their AAV tagging vectors (pAAV-SEPT-Acceptor...via gene trap enrichment, whereas pAAV-TK-Acceptor has a conventional heterologous promoter-driven TK-neoR...editing compared to CRISPR editing CRISPR, of course, has started a revolution in gene editing. However, while... through aberrant double strand break repair, it has proven more challenging to develop high efficiency...

...reprogrammed less efficiently, no such correlation has been found between the age of the patients from whom...potentially promote tumorigenesis in vivo. The phenomenon has been recorded in past gene therapy trials and remains...reprogramming factors To date, the mRNA transfection method has not been used successfully to derive iPSCs from PBMCs...However, reprogramming of PBMCs by episomal vectors has yielded hiPSCs at a low efficiency (0.001-0.03%) ...efficiency: the pCXLE episomal vector toolkit It has been shown previously that suppression of TP53 enhances...

...quantified by cell transduction assays. Wild-type AAV2 has been reported to have a near-perfect physical-to-...performed on all new viral vector preparations. Addgene has used this method to generally validate our viral ... Material (AAV SFM), which is an AAV sample that has been quantified by 16 labs around the world and can...Hindson et al., 2011, Hindson et al., 2013), which has been shown to be more precise and more repeatable...2011): 8604-8610.4. Hindson CM, Chevillet JR, Briggs HA, Gallichotte EN, Ruf IK, Hindson BJ, Vessella RL,...

...challenge of keeping up on studies in many disciplines has been a long brewing frustration for me. I read a ...citations in their own work. Of course not all research has to be influenced by research in other disciplines...platforms were developing, Google acquired Youtube and has been growing its online video market ever since. ...this point most videos are based on research that has been published, but many discuss in-progress research...videographer, is a retired botanist and oceanographer who has a blog and several videos on sharing your research...

...University of California system, the postdoc union has negotiated a raise from $43,692 to $48,216, an increase...increase in salary of 58.3%. University of Delaware has a minimum of $36,360, requiring an increase of 30.6%...Hospital is the only part of the Harvard group that has responded to the FLSA at the time of writing, and...good thing. Reliance on a cheap trainee workforce has clearly not been a sustainable approach to biomedical...biomedical research in the U.S. and postdoctoral training has not been demonstrated to be required for the jobs...

...into Reddit. Here are some reasons why: 1. Reddit has a large and active community of people interested...right - the Reddit community is active, engaged, and has great questions! 2. The r/Science subreddit is well...the internet, using dialogue). Luckily r/Science has a great team of moderators who facilitated scheduling...preferred time to start answering questions. r/Science has pretty strict rules about not being too sales-y in...the requested topics to the Addgene blog The blog has many other excellent posts on science career topics...