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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 405 results for: Lif;

Showing: 121 - 140 of 405 results

  1. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    Published
    Nov. 19, 2013, 2:37 p.m.
    ...recombination is the process by which nearly all domains of life repair genomic damage, specifically double strand...tags have been integrated. Simply design your amplification primers with the desired targeting homology—...

  3. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    Published
    Aug. 11, 2015, 2:30 p.m.
    ...understanding how nuclear organization varies across the life of a cell, or how organization may be altered in...Addgene. A protein-tagging system for signal amplification in gene expression and fluorescence imaging....

  4. Cre-ating New Methods for Site-specific Recombination in Drosophila

    Type
    Blog Post
    Published
    May 12, 2015, 1:32 p.m.
    ...from the standard UAS/GAL4 system is toxic to proliferating cells. A Cre-estrogen receptor ligand binding...PubMed. Reduction of Cre recombinase toxicity in proliferating Drosophila cells by estrogen-dependent activity...

  5. London Calling - Addgene’s New Europe Office in London

    Type
    Blog Post
    Published
    June 10, 2014, 3:20 p.m.
    ...the University of London and the University of California, specialising in Molecular Biology. She has worked...Genetics. Since then she has worked in the the UK, California, Africa, and Australia, viewing science as a ...

  6. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    Published
    April 2, 2020, 6:17 p.m.
    ...within a cell make them attractive tools for the life scientist or bioengineer.   Figure 1: Map...sequence used as an initiation point for PCR amplification or sequencing. Primers can be exploited for ...

  7. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    Published
    Sept. 14, 2017, 12:40 p.m.
    ...have increased thermostability also had a longer lifetime in human blood. To test if this was also true ...Lucas, et al. "A thermostable Cas9 with increased lifetime in human plasma." bioRxiv (2017): 138867. Additional...

  9. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    Published
    April 15, 2014, 3:11 p.m.
    ... people are paying attention to living a “green” life and creating less waste.  Most communities seem ...wasteful packaging.  Turns out, one could make a lifetime supply for about $10 US dollars worth of glass...

  10. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    Published
    March 9, 2017, 3:40 p.m.
    ...tenured faculty position for the grind of corporate life”.   In fact, there are a lot of good reasons to ...more structured time management conducive to work/life scheduling Diverse opportunities to do science related...

  11. RUBY-Red Siliques

    Type
    Blog Post
    Published
    March 1, 2022, 2:15 p.m.
    ... events and am currently planning to use it to simplify my work with an Arabidopsis mutant that can’t ...unfamiliar with these mutant plants. Using RUBY to simplify the identification of these mutants will speed...

  12. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type
    Blog Post
    Published
    Sept. 14, 2021, 1:15 p.m.
    ...that contain repeating elements. Knowing how to amplify recombination-prone plasmids and testing your prep...without affecting mRNA translation efficiency or half-life. RNA 25:507–518. https://doi.org/10.1261/rna.069286.118...

  14. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    Published
    Oct. 27, 2016, 2:30 p.m.
    ...insert comes from using Taq polymerase for the amplification step since Taq polymerase leaves a single deoxyadenosine... add unique restriction sites on the ends) and amplify your sequence of interest with Taq polymerase using...

  15. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    Published
    Aug. 25, 2015, 2:30 p.m.
    ...at 22. Start with your network You've spent your life making connections and finding mentors -- well, ... evolve and respond to feedback throughout its lifetime.  In this way, you will always improve and give...

  16. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    Published
    Feb. 20, 2018, 2:30 p.m.
    ...repair Adapter Ligation Clean up/Size selection Amplification Clean up  I found this kit easy to use because...DNA we started the process with, we got great amplification of our sample libraries. NEB also gave us a ...

  17. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    Published
    April 9, 2020, 1:15 p.m.
    ...viruses rely on the help of human proteins in their lifecycle, identifying these potential human helpers of ...Melanie Brewer (right) from the University of California, San Francisco. Manon Eckhardt and Melanie Brewer...

  18. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    Published
    Nov. 7, 2019, 2:18 p.m.
    ...at The Scripps Research Institute in La Jolla, California. She joined Elsevier after her postdoctoral studies...range of primary research and review journals across life and physical science, and editorial contribution...

  19. Science Career Options

    Type
    Blog Post
    Published
    Feb. 23, 2017, 1:30 p.m.
    ...overtime and weekends, part time or full time) and what life is like for someone who currently in the position...online job websites to see what requirements or qualifications are needed for the role, as this will give ...

  20. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    Published
    June 23, 2022, 1:15 p.m.
    ...saw these two papers,” said the University of California Berkeley’s Jennifer Doudna. “They are the first...continue to be a primary preoccupation of her California lab and many others. “Those off-target effects...
Showing: 121 - 140 of 405 results