Skip to main content
Addgene
Showing: 121 - 140 of 312 results

  1. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ...quickly. Of course, you might have less time to explore scientifically since you can’t afford to spend ...time working on something extremely risky or exploratory. Getting a job afterwards: Fairly or unfairly...

  2. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...ll dive into those questions in this post as we explore… the life and times of the myc tag.  Origins of...that their new monoclonals would help the field explore Myc’s role in cell proliferation and tumorigenesis...

  3. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ...remove RNA contamination and you can use a phenol chloroform extraction to remove remaining proteins and RNase...concentration of a chaotropic salt (guanidine hydrochloride for the plasmid purification protocol and guanidine...

  5. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ...but also alter an individual’s native, beneficial flora. Currently, there is no antibiotic strategy available...population, represented here by cells of different colors, specifically targets bacteria with the given genomic...

  6. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    Published
    May 15, 2014, 3:33 p.m.
    ...processes. Re-engineering GFP to increase its range of color and application Almost as soon as its sequence... not listed here, many permutations within each color also exist with only slight variations separating...

  7. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    Published
    May 2, 2019, 12:41 p.m.
    ...’ll choose to study Crepidula atrasolea or Prochlorococcus with your next project. Gymnotiformes and Mormyridae...evolution in immunology, neurobiology, and more. Prochlorococcus (Penny Chisholm) This cyanobacterium is the...

  8. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    Published
    April 11, 2017, 2:30 p.m.
    ... their selection techniques to come up with new colors (Fig. 3), such as Radish and Carrot, and further... ligands. References 1. Ma, Hanhui, et al. “Multicolor CRISPR labeling of chromosomal loci in human ...

  10. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ...other disciplines, or from other countries, to explore new connections to our research. So how can we ... 525.7569 (2015): 306-307. 3. Spicer, Scott. "Exploring video abstracts in science journals: An overview...

  11. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ...into the content of Addgene’s aptamer soup by exploring the development and applications of fluorescent...aptamers Just as vegetables come in a variety of colors, so do aptamers! Corn was recently described in...

  12. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    Published
    April 8, 2025, 1:15 p.m.
    ...experiment and choice of loading control. First, let’s explore why these assumptions are key to using loading ...loading control. Thanks for joining us in this exploration of western blot normalization. May your blots...

  13. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    Published
    Aug. 23, 2016, 2:30 p.m.
    ...Watch Joanne give her "Not" Networking 101 Talk 2. Tailor every talk to the occasion Don’t just plan to reuse...about the particular audience and venue before tailoring each presentation appropriately.  Find out who...

  14. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    Published
    Aug. 2, 2016, 2:30 p.m.
    ...specific nucleotide substitutions (or deletions) in a tailored manner. The approach can be used in conventional...plasmids, but may also facilitate genome editing. Tailored mutations are commonly introduced to endogeneous...

  15. Evolution of Lab Techniques

    Type
    Blog Post
    Published
    June 21, 2016, 2:30 p.m.
    ...fluorescence for fluorescently labeled probes. Colorimetric and luminescence based detection methods are...and behavior.     References 1. Southern, Edwin Mellor. "Detection of specific sequences among DNA fragments...

  16. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ...of carbohydrate binding proteins waiting to be explored and exploited. With better characterization, these...Jessica C. Sacher, and Christine M. Szymanski. "Exploring the interactions between bacteriophage-encoded...

  18. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    Published
    Feb. 25, 2020, 2:15 p.m.
    ... work The Island of Dr. Moreau (1896) where he explores the concepts of animal testing and modifications...but the common themes that these authors were exploring were space and robots. Warping to stars in space...

  19. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    Published
    Jan. 19, 2021, 2:15 p.m.
    ...antibodies (i.e., immunofluorescence), or by a colorimetric reaction facilitated by an enzymatic reaction...Cruse JM, Lewis RE, Wang H, Geziena MT, Steven GE, Lorna J. (2004) ANTIGENS, IMMUNOGENS, VACCINES, AND IMMUNIZATION...

  20. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ...effector — Cas9, Cas12, or Cas13. If you’d like to explore Class 2 CRISPR in more depth, the Jennifer Doudna...8(1). https://doi.org/10.1186/s13578-018-0255-x Taylor, H. N., Laderman, E., Armbrust, M., Hallmark, T...
Showing: 121 - 140 of 312 results