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We narrowed to 675 results for: Mos;

Showing: 121 - 140 of 675 results

  1. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    Published
    Nov. 7, 2014, 2:56 p.m.
    ...are the most common examples of the E. coli B strain. Common E. coli strains used in the lab Most of the...that make these features possible are present in most commercial strains, especially mutations that make...Streptomycin Hybrid of E. coli K12 and E. coli B (mostly K12, though), common lab strain for cloning and...covering E. coli and others are model organisms! Our Most Popular Plasmids 101 Posts: Origin of Replication...

  2. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ...research is rooted in the CNS, my focus will be mostly on the brain, but many of these tips apply to all... estate and using AAVs have in common? The three most important items when looking for a house and choosing...Well, an AAV user must determine which serotype is most efficient at transducing the cell type under study...influences on overall cell function. AAVs are the most appropriate vehicle for this type of research, as...zinc finger nucleases, and many others (12). The most attractive potential of AAVs is their ability to...

  3. Finding Your Science Policy Path

    Type
    Blog Post
    Published
    Sept. 22, 2020, 1:15 p.m.
    ...the way. That’s what makes it so wonderful. The most challenging part of jumping into this career path...explainer of the who, what, where, when, why and most importantly, how, of a science policy career.  Who...engagement with your local or state governments. Most communities have opportunities to join commissions...do science policy? I would argue that this is the most important question that you can explore. The answer... important skills for the profession. One of the most important skill sets for a science policy career...

  4. 6 Tips for Analyzing and Troubleshooting Sanger Sequencing Results

    Type
    Blog Post
    Published
    June 26, 2014, 2:23 p.m.
    ... pass on to you to make sure you are getting the most out of the data you get back from your sequencing...controls help you properly visualize your results.The most important of those is to always look closely at ...miscalled peak, don’t be shy. Feel free to edit it. Most chromatogram viewing programs (even the free ones...sequence.   I hope these tips will help you get the most out of your DNA sequencing results and to troubleshoot...

  5. Your Top Requested Plasmid in 2016!

    Type
    Blog Post
    Published
    Jan. 11, 2017, 8:47 p.m.
    ...become. One thing is for sure, as is obvious from our most requested plasmid, SpCas9 is still going strong ...editing experiments. So, without further ado, the most request plasmid in 2016 was... pSpCas9(BB)-2A-Puro... lines. Cas9, of course, is the key component of most CRISPR systems and generates double strange breaks...name are some additional features. The first and most imporant is that, upstream of the Cbh promoter, ...

  6. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    Published
    June 28, 2018, 3:01 p.m.
    ...Centre for Regenerative Medicine (CRM), Edinburgh. Most commercial antibodies do not work in pull-down assays...diverse biochemical tasks in the cell. One of the most popular approaches to study protein interactions...methods (electroporation, lipofection etc) is the most crucial aspect of knock-in experiments. You’ll need...depends on many factors. Some of these include: Most importantly, the crRNA should cut near the insertion...with custom synthetic cr:tracrRNAs (csRNP) is the most efficient and convenient choice for epitope tagging...

  7. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    Published
    July 9, 2015, 2:30 p.m.
    ... network of relationships early and often is the most effective tactic a scientist in training can adopt...professional (and personal) relationships. However, most job seekers will and should apply for jobs posted...get help if you are having trouble preparing your most excellent resume. 2) Follow job boards and check...letters that expound on bench skills and experience. Most Addgene PhD level job descriptions clearly describe...

  8. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    Published
    June 4, 2020, 1:22 p.m.
    ...helix, fragmenting plasmid DNA, PCR product, or chromosome. One of the main causes of degradation during...endonuclease I encoded by the gene endA. Nowadays, the most common E. coli strains used for plasmid amplification...preparation stabilizing the plasmid, and still allowing most downstream processing. On the other hand, nuclease...where you plan to store it and for how long. The most common solution is to keep your plasmid at -20°C...

  9. FastCloning: A Newer, Simpler Cloning Technique

    Type
    Blog Post
    Published
    Nov. 1, 2016, 2:30 p.m.
    ...alteration. Molecular cloning techniques are some of the most widely used techniques in the biological sciences...Although each type of cloning has its advantages, most scientists still encounter several struggles with... overhangs, insert and vector purification, and, most importantly, excessive time requirements. FastCloning...polymerases like phusion. An 18-cycle PCR leads to the most colonies after transformation. When a 1:1 vector...

  10. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    Published
    April 15, 2014, 3:11 p.m.
    ...less waste.  Most communities seem to have recycling programs and it seems to me that most Addgenies make...advent of techniques like PCR and DNA synthesis. Most molecular biologists bought the pricey stuff in ...only Addgenie who has a thrifty streak.  One of the most accessed blogs on BitesizeBio is the protocol for...

  11. The Strength of Story Telling

    Type
    Blog Post
    Published
    Dec. 19, 2017, 2 p.m.
    ...advantage of that in your work The part of my job I most enjoy is building and telling interesting stories...following cause-and-effect relationships are all things almost all of us are programmed to appreciate and understand...had a conflict at work.” Interviewee: “I did the most work for a paper we were publishing, but when it...rethink your structure. Develop your protagonist In most cases, the protagonist will be you. Make sure to...

  12. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    Published
    June 9, 2022, 1:15 p.m.
    ...podcasts, science pub nights, and pop sci books. Most scicomm efforts fall under informal learning; however...disease testing, it can still be considered jargon in most other uses. Many laypeople would use “PCR test” ... rather quickly!  The question   The first, and most important question, you should ask for any science...In fact, it’s really the opposite. Unlike emails, most science communication work does not explicitly exclude...

  13. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type
    Blog Post
    Published
    Oct. 29, 2015, 2:30 p.m.
    ...exist either autonomously or integrated into the chromosome” was proposed a few years later by Élie Jacob... 1952 was noted to integrate into the E. coli chromosome in some cases. This terminology held until the...1960s when scientists began to study other extrachromosomal particles, particularly Resistance or R-factors... idea that R-factors could integrate into the chromosome. Thus the term “episome” was eventually dropped...

  14. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    Published
    Aug. 21, 2014, 3:15 p.m.
    ...sales and marketing jobs Like most choices we make, my job choices were mostly by chance. Luckily, around...but also my business acumen and sales experience. Most scientists think that they are unable to tick these...both my scientific and marketing knowledge. For most PhDs looking at careers outside of academia, sales...

  15. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    Published
    Jan. 13, 2025, 9:49 p.m.
    ... to correct only a minority of those variants in most cell types. But prime editing, a CRISPR technique...efficiency of HDR is relatively low, meaning that most Cas9-induced DSBs are repaired by non-homologous... end joining. As a result, Cas9 treatment causes most products to be indels while the efficiency of HDR...application, and the efficacy varies widely — it still almost always results in higher ratios of desired edits...binding site, increase processivity, or improve thermostability. What came out on top? The Cas9 nickase fused...

  16. The MTA at Addgene

    Type
    Blog Post
    Published
    July 23, 2024, 2:17 p.m.
    ...Material Transfer Agreement (UBMTA) is one of the most commonly used ones for transfers between academic...research use agreements. The UBMTA, which is the MTA most plasmids at Addgene are shared under, is well-understood...the majority of research institutes.  Pro tip! In most academic and nonprofit institutes, the Tech Transfer... to share your biological materials under! While most plasmids at Addgene are shared under the UBMTA, ...

  17. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    Published
    Feb. 20, 2018, 2:30 p.m.
    ... stands for Fragmentation System and this is the most important part of this newly released prep kit. ...insulated glass to take home with us. Tips to to get the most out of a workshop Although we probably won’t use...the exact way we did at the workshop (the kit is mostly used to make new libraries as opposed to replicating...workshop because it will help you think about the most useful pieces of information from the workshop. ...

  18. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    Published
    June 6, 2024, 1:50 p.m.
    ... and after compensation (C) with APC-Cy7.   FMOs FMO stands for fluorescence minus one. It is used...used in the later gates, are more likely to require FMOs. Isotypes Isotype controls are negative controls...daunting process. Controls like single color controls, FMOs, and isotypes can help you more easily gate your...will help you save on sample volume, so you can use most of your samples for flow analysis rather than for...

  19. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    Published
    July 25, 2023, 1 p.m.
    ... on Addgene.org Addgene's most popular lentiviral plasmids Addgene's most popular retroviral plasmids...discuss them together. Gene expression The reason for most viral experiments is to deliver a genetic cargo,...Fun fact: There are 57 human adenovirus types, but most vectors are based on adenovirus 5 (Ad5).   ...

  20. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    Published
    Feb. 1, 2022, 2:15 p.m.
    ...signaling molecules. Assay Type Westerns and ELISAs mostly use HRP, a chemiluminescent conjugate that can ...however, is pretty much every other assay you can do. Most non-HRP conjugates are fluorophores, which work ...be detected in the same assay. This is one of the most complex subjects in antibody-based protocols - but... them whenever possible. This will allow for the most accurate read and reduce needed compensation.    ...
Showing: 121 - 140 of 675 results