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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 329 results for: NUC

Showing: 121 - 140 of 329 results

  1. RNA Extraction Without A Kit

    Type
    Blog Post
    Published
    March 24, 2020, 1:15 p.m.
    ... of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 18:5294–5299...effect of ammonium sulfate on the activity of ribonuclease A. Biochimica et Biophysica Acta (BBA) - Enzymology...

  2. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ... As a genome conversion in the transduced cell nucleus, the single-to-double stranded conversion of the...of symmetry. After uncoating in the target cell nucleus, this DNA structure can readily fold into transcriptionally...DNA folds back on itself to become dsDNA in the nucleus and is a good option if you need rapid transgene...

  3. All in a Twist: dsRNA

    Type
    Blog Post
    Published
    Feb. 2, 2023, 2:15 p.m.
    ...rarely circulates, but upon viral infection, this nucleic acid can build up. The foreign dsRNA activates ...Transposable elements such as long/short interspersed nuclear elements can generate dsRNA by bidirectional transcription...constantly occurring throughout a single cell, so these nucleic acid hybrids are always around. R-loops are particularly...

  4. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    Published
    Feb. 28, 2017, 3:30 p.m.
    ... and its organization within the nucleus, also called the 4D nucleome. Visualizing specific loci has historically...loci, a major benchmark in characterizing the 4D nucleome. CRISPRainbow: Scaling up to 6 colors + white ...

  5. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    Published
    July 23, 2020, 1:20 p.m.
    ...DNA cleavage Example: AcrIIC1 binds to the HNH endonuclease domain of Cas9 and prevents target DNA cleavage...off-target edits of base editors, which convert a nucleotide base pair to another base pair at a specific ...effects and cytotoxicity that result from excessive nuclease activity in tissues (Li et al., 2018). 4. Selection...

  6. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ...protein – but were very confused because the HNH nuclease active site wasn’t even close to its reported ... DNA strand, and then activate two different endonuclease active sites. Fortunately, there is never just...displaced non-target DNA strand and shows the HNH nuclease domain swung around in close juxtaposition to ...

  7. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    Published
    Sept. 12, 2017, 1:44 p.m.
    ...someone grabs the wrong plasmid? What if bacteria snuck into the prep during the production process? These... Each contig is then BLASTed against the NCBI nucleotide collection database, and it may also be aligned...Next-generation Sequencing. Molecular Therapy - Nucleic Acids 4:e260 . https://doi.org/10.1038/mtna.2015.32...

  8. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    Published
    Aug. 4, 2016, 2:30 p.m.
    ...stage with much-lauded CRISPR-Cas9 is DNA-guided endonuclease NgAgo! We'll discuss how NgAgo is faring with...mammalian cells and one which diminishes the endonuclease activity of the enzyme. In vivo, only nascent...someone came up with a mutant NgAgo (or a novel endonuclease) protein that can form a complex with a 5’-P-ssDNA...

  9. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    Published
    May 22, 2015, 4:58 p.m.
    ...to insert an endonuclease gene within its own cut site1. In heterozygotes, the endonuclease cuts the wild-type...ensuring that all future offspring inherit the endonuclease gene. As we pointed out last year2, delivering...

  10. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...editing with various spacer lengths from 30-84 nucleotides, and designated this system REPAIRv1. Using next-generation...had A-to-I off-target edits around the targeted nucleotide. Introduction of guanine-mismatches in the guide... at Addgene. Gootenberg, Jonathan S., et al. "Nucleic acid detection with CRISPR-Cas13a/C2c2." Science...

  11. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    Published
    Oct. 4, 2018, 12:44 p.m.
    ...commonly result in a complete loss of function while nuclease-deactivated Cas9 (dCas9) can be tethered to a ...which contains multiple point mutations in the nuclease domain. These mutations result in an inactive ...CRISPRa, sgRNAs were targeted between 150-75 nucleotides upstream of the TSS. Similar to the Brunello ...

  12. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ...on the size of the knock-in. Single strand oligonucleotide donors (ssODNs) with homology arms as short...need to be used due to synthesis limits of oligonucleotides. These donors traditionally have larger homology...Cas break for HR is to fuse an HR protein to the nuclease. Early HR factors fused to Cas9 have been shown...

  13. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    Published
    Oct. 22, 2015, 2:30 p.m.
    ...modification, protein degradation, access to the nucleus in eukaryotic screens and fusion configuration....cells, fusion proteins must gain access to the nucleus. To circumvent this requirement for transmembrane...system for the pathogenic fungus Candida albicans. Nucleic Acids Res. 2010;38(19):e184. Pubmed PMID: 20719741...

  14. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    Published
    Oct. 13, 2022, 1:45 p.m.
    ...many cases several exons’ worth of DNA. Any ∼20 nucleotides of DNA can serve as the target for Cas9’s single...specific? Perhaps you need to introduce a single nucleotide modification at a defined amino acid to mimic.... & Liu, D. R. Genome editing with CRISPR–Cas nucleases, base editors, transposases and prime editors....

  15. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    Published
    Dec. 7, 2021, 2:15 p.m.
    ...target protein interacts with other proteins or nucleic acids. Read on to learn more about this versatile...difficult to release proteins, including those in the nucleus, more stringent buffers may be needed. Since many...also be used to study proteins that interact with nucleic acids. Labs studying epigenetics routinely use ...

  16. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ... of the tRNA contains the anticodon,  a three nucleotide sequence complementary to the mRNA sequence. ... sequence databases: status for the year 2000. Nucleic Acids Research 28:292–292 . https://doi.org/10.1093...codon usage bias, and its potential applications. Nucl Acids Res 15:1281–1295 . https://doi.org/10.1093...

  17. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    Published
    May 8, 2018, 1 p.m.
    ... the ICE software tool soon. To request other nucleases or other features and functionalities, please ...Guide Target - This is the user-defined 17-23 nucleotide sequence of the DNA-targeting region of the guide...Protospacer Adjacent Motif (PAM) sequence for the nuclease used. Currently, ICE is configured for Cas9 fromStreptococcus...

  18. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    Published
    Nov. 3, 2022, 12:15 p.m.
    ...can deliver a silent mutation in a neighboring nucleotide along with your desired edit. This silent mutation...editing by sequence trace decomposition. (2014) Nucleic Acids Research Volume 42, Issue 22, Page e168. ... template-directed CRISPR-Cas9 editing. (2018) Nucleic Acids Research Volume 46, Issue 10, Page e58. https...

  19. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type
    Blog Post
    Published
    Sept. 5, 2017, 1:58 p.m.
    ...end-joining (NHEJ) pathway, leading to nonspecific nucleotide insertions, dele­tions or other mutations, referred...repair template, such as a sin­gle-stranded oligodeoxynucleotide (ssODN), which contains the intended sequence...How to Design Your gRNA Delivering CRISPR Ribonucleoproteins Resources on Addgene.org Browse All CRISPR...

  20. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    Published
    June 17, 2025, 1:15 p.m.
    ...hairpin shRNA structures that are processed by a ribonuclease called Dicer. In a great simplification of the...promoter Mature siRNAs typically range from 19–22 nucleotides and are 100% complementary to their target sequence...assumptions about miRNA-guided gene silencing. Nucleic Acids Research, 50(2), 617–634. https://doi.org...
Showing: 121 - 140 of 329 results