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We narrowed to 447 results for: PAN

Showing: 121 - 140 of 447 results

  1. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type
    Blog Post
    Published
    Nov. 5, 2019, 1:28 p.m.
    ...Our previous blog post The PAM Requirement and Expanding CRISPR Beyond SpCas9 described the PAM specificity...toolkit available to researchers will continue to expand. Final thoughts Since the first use of Cas9 to ..., et al. "Engineered CRISPR-Cas9 nuclease with expanded targeting space." Science 361.6408 (2018): 1259...

  2. Clear and Accessible Writing

    Type
    Blog Post
    Published
    Feb. 15, 2024, 2:15 p.m.
    ...wait!  Done? Excellent! We asked our workshop participants to do the same thing, and even though only about...Figure 1, Addgene chose this alt text: "The left panel is a profile photo of an iodixanol gradient after...passage of supernatant through each layer. The right panel illustrates a gradient tube before and after ultracentrifugation...

  3. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    Published
    July 22, 2014, 5:46 p.m.
    ...previous blog post about how they started their company here. The first transgenic plant was engineered...distribution. We hope that will change with Addgene’s expansion into plant synthetic biology. There are already...the years were generated by large agricultural companies and carry associated intellectual property concerns...

  4. RNA Extraction Without A Kit

    Type
    Blog Post
    Published
    March 24, 2020, 1:15 p.m.
    ...aqueous layer is extracted and RNA can then be isopropanol precipitated. This method reduced the time it...or tissue collection method, so biotechnology companies have marketed several products that help to further... RNAzol®, QIAzol®  and sold by many different companies). TRIzol® is an all-in-one acid-guanidinium-phenol...

  5. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ...enzyme. Fast facts – PAM requirement: NGG and an expanding number of others. Best for: a wide range of DNA...scientist toolbox. If your genome engineering goals expand beyond generating frameshift mutations or single...my CRISPR experiment? The PAM requirement and expanding CRISPR beyond spCas9 Cas proteins More resources...

  6. 6 Tips for Grant Writing

    Type
    Blog Post
    Published
    Oct. 18, 2016, 2:30 p.m.
    ...someone senior from accounts or HR on the judging panel. It’s important for this person to get a clear and...scoring system or meeting with their grant submission panel. Some funding bodies provide opportunities to attend...entrepreneurial bug and has set up an ELISA kit company on whose website you can find some great information...

  7. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    Published
    June 5, 2014, 3:32 p.m.
    ...Revolution Bioengineering.  Last year we started a company. Revolution Bioengineering is two responsible adults... this technology and angry at the agriculture companies that produce GMO food plants. When a press piece... of our business, because we are building this company, and that’s what we want it to do. It’s exhilarating...

  8. Antibodies 101: Flow Compensation

    Type
    Blog Post
    Published
    May 16, 2024, 1:15 p.m.
    ...know if you will need to compensate in your flow panel, you will need to know both the fluorescent markers...positive APC population is circled in red in both panels.    Pro tip! To determine which way your population...compensation between APC and APC-Cy7, with compensation panels below each plot.  The general rules of compensation...

  9. PhD Applications After COVID

    Type
    Blog Post
    Published
    Jan. 5, 2023, 2:15 p.m.
    ...without the financial and logistical stress that accompanies traveling for campus visits. This is definitely... technologies that helped us navigate a global pandemic can also be the thing that provides a new perspective...developed virtual environment resulting from the pandemic, students who are unable to attend in-person interviews...

  10. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...This post was contributed by Jae Lee and Pantelis Tsoulfas of the Department of Neurological Surgery ...  Thank you to our guest bloggers, Jae Lee and Pantelis Tsoulfas. Jae K. Lee is an assistant Professor...Medicine. Find plasmids from the Lee Lab here. Pantelis Tsoulfas is an Associate Professor at the University...

  11. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    Published
    Feb. 9, 2023, 2:15 p.m.
    ...hypotheses being rejected, etc.), while reminding participants of the value of this approach and encouraging...by making a greater number of students active participants in the research process – so if this sounds ...classroom-based undergraduate research experiences spanning chemical biology, biochemistry, and neurobiology...

  12. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    Published
    Oct. 6, 2015, 2:30 p.m.
    ...is ideal for tracking large collections. Many companies, such as Thermo Fisher and Micronic, provide 2D... time. 1D barcodes can be purchased from many companies such as Corning. Tubes can even have specific ...specific barcodes etched or printed onto them by companies such as Tubewriter. Any lab can benefit from using...

  14. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...NGS) technology. As our repository continues to expand, we realized the need to modernize our approach... number of deposited plasmids increased, the accompanying sequencing data collection and analysis eventually...Jupyter notebooks using familiar libraries such as Pandas, Numpy, and Biopython.  We started at the usual...

  15. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ...Nucleocapsid Protein antibodies #211756 (left panel) or #211757 (middle panel). Samples were also immunoblotted against... against vinculin as a loading control (right panel). Image from Addgene. These antibodies are recombinant...

  16. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    Published
    Jan. 3, 2019, 1:35 p.m.
    ...plant base editors. Plant CBE and ABE with expanded PAM compatibility All of the base editors mentioned.... Hua, Kai, Xiaoping Tao, and Jian‐Kang Zhu. "Expanding the base editing scope in rice by using Cas9 variants.... PubMed PMID: 29867128. 11. Li, Chao, et al. "Expanded base editing in rice and wheat using a Cas9-adenosine...

  17. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type
    Blog Post
    Published
    July 7, 2020, 1:15 p.m.
    ...their previous reporter, the Harris Lab created a panel of eGFP reporters to quantify the on-target DNA ...other enzymes such as Cre-recombinase drastically expanding the base-editing reporter toolbox. The Dow lab...Serebrenik AA, Shaban NM, Brown WL, Harris RS (2019) A panel of eGFP reporters for single base editing by APOBEC-Cas9...

  18. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...and Pandey et al., 2023 under CC-BY 4.0 License. Find PE6 plasmids here!  Doman, J.L., Pandey, S....antibodies can be tethered on the viral surface, expanding the range of targetable cell types. DIRECTED is...

  19. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ...Young. The use of CRISPR/Cas9 for gene editing has expanded since its adaptation for use in mammalian cells...ordering the sgRNA is simple and easy. Several companies supply gRNA sequences, such as GenScript, ThermoFisher...order them yourself through whatever DNA supply company you currently use. You can also find many previously...

  20. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    Published
    Nov. 16, 2021, 2:15 p.m.
    ....  How do you find validated antibodies? Many companies that produce and distribute antibodies validate...through their publications or as a review on a company website. It is important to review the data and...can help you compare antibodies from different companies, such as Validated Antibody Database and CiteAb...
Showing: 121 - 140 of 447 results