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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 290 results for: Pol;

Showing: 121 - 140 of 290 results

  1. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    Published
    May 9, 2017, 2:12 p.m.
    ...Cassettes transfection gRNAs expressed from a polycistronic transcript  Requires co-transfection of Cas9...Cassettes transfection gRNAs expressed from a polycistronic transcript Requires co-transfection of Cas9  ...

  2. The time and cost required to make a plasmid

    Type
    Blog Post
    Published
    March 23, 2023, 1:15 p.m.
    ...plasmid? Figure 2: Survey results from a Twitter poll run in 2023, n=194.   After the great Plasmid...much harder to calculate, in both our 2018 and 2023 poll, most people said it took them more than 22 hours...

  3. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    Published
    Oct. 1, 2019, 1:21 p.m.
    ...shoulder for the picture. It tends to look like a police photo if you look straight at the camera. Pro tip...organizational work. LinkedIn is not the platform for politics, family updates, or click bait of any kind. For...

  4. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    Published
    Oct. 20, 2015, 2:30 p.m.
    ...professor of economics at the Andrew Young School of Policy Studies at Georgia State University, is an expert... in the intersection between science and public policy. Follow David on Twitter @driglar.     References...

  5. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    Published
    Nov. 14, 2024, 2:15 p.m.
    ...and Bester 2021). For example, inserting a polyadenylation signal downstream of the transcription start...fragment. (D) Knock-in: insertion of a synthetic polyadenylation (spA) signal. Figure and caption adapted from...

  6. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    Published
    Aug. 9, 2022, 1:15 p.m.
    ...). mAbs and rAbs have higher homogeneity than polyclonal antibodies because of their immunoglobin component...circumvents the batch-to-batch variability found in polyclonal antibodies, creating a potentially unlimited ...

  7. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    ...such as the serotonin 3 receptor, will promote depolarization in neurons. Alternatively, combining with the...such as the glycine receptor, will promote hyperpolarization. PSAM ligands The advantage of PSAMs lies ...

  8. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    Published
    Aug. 7, 2018, 12:31 p.m.
    ...In addition the transfection method (i.e. polyethyleneimine, calcium phosphate or liposomal methods), ...centrifugation on a sucrose gradient, spin columns, and polyethylene glycol (PEG) precipitation. All of these methods...

  10. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    Published
    Nov. 19, 2015, 3:30 p.m.
    ...can influence the transcription of genes by RNA polymerase II, or by the addition of methyl- groups to the...9 methylation, while the K27 reporter uses the polycomb chromodomain to recognise lysine 27 methylation...

  11. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    Published
    May 23, 2019, 12:26 p.m.
    ...conference If the conference has a social media policy, this should be on their website or the program...program booklet. Make sure you’re familiar with the policies before posting something on social media. If someone...

  12. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    Published
    Feb. 25, 2016, 3:30 p.m.
    ... myidp.sciencecareers.org mentioned museums. Indianapolis is home to one of "The world’s biggest and best...children’s museum", The Children's Museum of Indianapolis. I drove passed these dinosaurs every day. They...

  13. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...single mRNA. IRES was originally discovered in poliovirus RNA, where it promotes translation of the viral...eukaryotic mRNA directed by a sequence derived from poliovirus RNA. Pelletier et al (Nature. 1988 Jul 28;334...

  14. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...While monoclonal and polyclonal antibodies are readily available from several sources, fewer sources ...Antibodies Plasmid-based Recombinant Antibodies Polyclonal Antibodies Resources on addgene.org Addgene's...

  15. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    Published
    July 30, 2020, 1:15 p.m.
    ... challenges of science becoming increasingly politicized. It would be nearly impossible for a career scientist...science in a “fake news and post-truth era,” the politicization of science (from climate change deniers to ...

  16. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    Published
    Feb. 6, 2020, 3 p.m.
    ...relevant parties, and the company at large, by polling and informal communications we made a decision ...scientists often prefer data. At Addgene we rely on polls and surveys. A mix of closed (multiple choice/binary...

  17. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ...allows for the following to occur: A) Multiple polyclonal secondary IgGs can bind to different epitopes... Reagent Pros Cons Antibodies Bivalent Polyclonal Can be labeled with multiple dyes Commercially...

  18. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    Published
    Sept. 28, 2017, 2:01 p.m.
    ...CREATE cap plasmid, loxP sites flank the downstream polyA sequence. When viruses produced from this plasmid...into an animal with cell-type-specific Cre, the polyA sequence is inverted only in the Cre-expressing ...

  20. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ...ensure this tRNA processing only occurs on the RNA polymerase III transcript, the sgRNAs were encoded on the...donor site; SAS, splice acceptor site; pA, polyadenylation signal. Image reused from Riedmayr et al. 2023...
Showing: 121 - 140 of 290 results