We narrowed to 342 results for: SEF;
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TypeBlog PostPublishedJuly 12, 2016, 2:30 p.m....-targeting field. Mice have long been extremely useful in the lab – they are relatively small and easy...generating mouse models Although CRISPR is incredibly useful for generating mutations by NHEJ and generating...
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27 Hot Plasmids from 2016
TypeBlog PostPublishedDec. 22, 2016, 3:03 p.m....the Liu lab’s OXfCRISPR page provides many other useful resources for fly labs who wish to harness this... complex genetic elements. To demonstrate the usefulness of GAMP, the Jacks Lab constructed several GMAP-compatible...enough variation in a native genomic context to find useful mutants. CRISPR-X, a new system from Michael Bassik... -
Important Considerations When Using AAVs
TypeBlog PostPublishedJune 13, 2017, 2:30 p.m.... opted for AAV. While these benefits make AAVs useful for a wide variety of experiments, care still needs...improvements are being made to make them even more useful for therapeutic purposes. The University of Pennsylvania... -
Quick Guide to All Things Lentivirus
TypeBlog PostPublishedMarch 21, 2017, 2:30 p.m...., lentiviral vector constructs have proven very useful due to their ability to infect both dividing and...instance. I hope you will find this information useful for your experiments. Don’t hesitate to check our... -
Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)
TypeBlog PostPublishedMarch 7, 2017, 3:30 p.m....Targeting different regions of the gene is also useful in case the gene has a weird mutation that you ...digested product may be limited and faint, so it’s useful to have as many controls as possible to confirm... -
Live and Let Dye: Self-Labeling Protein Tags
TypeBlog PostPublishedApril 16, 2024, 1:15 p.m....bit of chemistry magic to produce a wide range of useful molecules linked to that substrate. If the substrate...green structure represents a fluorophore or other useful ligand, gray structure is the protein of interest... -
Custom CRISPR Screens & the Green Listed Software
TypeBlog PostPublishedJuly 11, 2017, 2:30 p.m....of genes provided by the user. This approach is useful for several applications as will be discussed below...different ligands. Such knowledge could be very useful for drug discovery, to dissect a complex signaling... -
Plasmids 101: Modular Cloning Applications and Kits
TypeBlog PostPublishedMay 14, 2024, 1:15 p.m.... and kits. Keep in mind that this technology is useful for a variety of purposes beyond what we discuss...cell-free systems. The approach is particularly useful for producing drug precursors, biofuels, and natural... -
Antibodies 101: Introduction to Antibodies
TypeBlog PostPublishedJan. 19, 2021, 2:15 p.m....antibody, and how is a component of the immune system useful as a research reagent? Let’s find out! What is ... The ability of antibodies to bind proteins is useful for research applications as well because they ... -
The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs
TypeBlog PostPublishedNov. 3, 2015, 3:30 p.m....definitions. These newer tools may still prove somewhat useful for the latest NTT targeting Cas protein, Cpf1....entirely necessary but should be considered extremely useful, depending on design goals. Examples: “feature... -
Deep Dive: qPCR
TypeBlog PostPublishedSept. 29, 2022, 1:15 p.m....forward or the reverse primer. There is a list of useful primer design resources in the References and Resource...spreadsheets available for easy analysis. These useful tools don’t excuse you from understanding how the... -
Technical Design of a Western Blot
TypeBlog PostPublishedAug. 22, 2024, 1:15 p.m....get a single-percentage gel, which is cheap and useful when you’re looking at proteins all roughly the...your choice of detection methods. They are very useful for troubleshooting or optimizing western blots... -
New Antibody Animation!
TypeBlog PostPublishedFeb. 22, 2024, 3:13 p.m....'s YouTube channel for more helpful animations, useful protocol videos, and quick tips for lab success... -
Plasmids 101 eBook 4th Edition
TypeBlog PostPublishedSept. 5, 2023, 1:15 p.m....fresh new look. We think this is a helpful and useful resource — but you don't have to take our word ... -
The 12 Days of CRISPR: 2021
TypeBlog PostPublishedDec. 28, 2021, 2:15 p.m....tweets featuring the best of CRISPR in 2021 and useful resources, fear not! You can find all of them here... -
SciComm with the Experts at Science in the News Part 1
TypeBlog PostPublishedMarch 16, 2017, 1:06 p.m....promote public support for research and to keep useful discoveries coming. At Addgene, we’re huge supporters... -
New Educational Resource: The Addgene Videos Page
TypeBlog PostPublishedJune 16, 2017, 2 p.m.... their career paths, future goals, and get some useful career advice. Our first video, featuring Outreach... -
Introducing Addgene's Microbiology Pages
TypeBlog PostPublishedJune 13, 2016, 7:08 p.m....form and we’ll take care of the rest. Know of a useful plasmid that’s not in the collection? Tell us about... -
Addgene Protocols Go Viral: AAV Purification and Titration
TypeBlog PostPublishedJune 13, 2019, 12:19 p.m....accelerate research and discovery by improving access to useful research materials and information,” we love to... -
Call for Guest Bloggers
TypeBlog PostPublishedMarch 2, 2016, 3 p.m....might consider are: An overview of software tools useful to any molecular biologist Tips for protein purification...