...levels for 6 days - after just 15 minutes of FUS.  Similar constructs to the one above can be made for expressing...But why might you need a second GFP antibody?  Similarities:  Generated against full length GFP and converted...the group rationally designed GFP variants with similar capabilities. If you’re looking for a super stable...

...disappears from the video, and prepares a coffee, or simply moves away from the desk to switch off a few minutes...GA, O’Reilly CM, Lofton ME, Catalán N (2018) Ten simple rules for collaboratively writing a multi-authored...journal.pcbi.1006508 Vicens Q, Bourne PE (2007) Ten Simple Rules for a Successful Collaboration. PLoS Comput...

...TCSs have been identified in bacteria, and with similar optimization methods, many of these systems could...see light. Levskaya A, Chevalier AA, Tabor JJ, Simpson ZB, Lavery LA, Levy M, Davidson EA, Scouras A, ...genetic edge detection program. Tabor JJ, Salis HM, Simpson ZB, Chevalier AA, Levskaya A, Marcotte EM, Voigt...

...proteins they identified have little sequence similarity to previously identified Cas13a-c orthologs, ...mammalian system. Since Cas13d is functionally similar to previously discovered Cas13 enzymes - what makes...their cleavage pattern: EsCas13d produced very similar cleavage products even when guides were tiled across...

...promoter and ceases repression of the target gene. A simplified lac inducible system is found in many bacterial...between cell types or organisms, promoters are similarly variable. Bacterial promoters only work in prokaryotic...related species from which they were derived. Similarly, the various eukaryotic cell types (mammalian,...

...create a genome-wide library capable of performing similarly to Cas9 screens in a compacted library. Overall...genes within a single cassette provides increased simplicity for combinatorial genetic screens, and this optimized...Penn State Protein Ladder  by: Rachel Leeson This simple, inexpensive plasmid-based ladder system is a cost-effective...

...Identifying the Issue You can’t fix an experiment by simply running it unchanged over and over again, and the...was not trying even harder to start earlier, but simply moving all my prep work to the day before, which...remember what's been added and what hasn't. Developing simple habits like these can consistently reduce errors...

...conjugate that can be activated to emit light with a simple kit, and is readily available on any number of ...ain’t broke, don’t fix it. HRP is easy to find, simple to use, not sensitive to light in its inactive ... this is an Antibody 101 post, so we'll keep it simple. If you follow the two basic guidelines below, ...

...facilitates the reproduction of the structure in a similar way to the biological protocol, and assembly designs... young movement of sharing open hardware has a similar goal. The sharing of modules, ideas, and blueprints...Nibha Akireddy, Chew Chai, Manu Prakash, "Squid: Simplifying Quantitative Imaging Platform Development and...

...suppressor screens These types of screens are very similar to forward and reverse screens, except that they... which are worth considering. RNAi screens are similar to CRISPR, but instead of knocking out the genes...temperature to exacerbate a mutant phenotype, very similar to enhancer/suppressor screens. Selections Selections...

...system work? Mendenhall: The system is really quite simple but it offers improvements in efficiency and throughput...right thing to do to put these out there. It’s a simple solution. It would be silly not to share it as ...on many other labs that may be doing something similar. There may be other variants of this that are better...

...together, thus repairing the break. This is a simplified, streamlined version of this pathway and does...regions of hundreds of base pairs or more can simultaneously introduce a pair of chromosome breaks, and ...deletions) if NHEJ joins the distal ends together. Similarly, it may be possible to direct insertion of an ...

...rate as compared to a constitutive promoter. To simulate the increase in plasmid copy number during the...expression from the constitutive promoter did not. Similar levels of expression between a plasmid and the ...stabilized promoter, deoxychromoviridans titers were similar regardless of where the genes were expressed. What...

...threonine kinase with a similar 3D structure to MEK1, but Raf1 and CDK5 have less similarity. For these three...versions of MEK1, MEK2, Raf1, and CDK5 all behave similarly to their endogenous counterparts. Additionally...

...absorb the excitation light less efficiently. Similarly, decreasing the power of the excitation light ...results in a loss of fluorescence, which, in its simplest form, can be described by a mono-exponential decay...intensity decay of a fluorophore does not follow a simple mono-exponential decay (Shaner et al, 2008; Bindels...

...cloning system - a Gateway compatible approach to simultaneously clone two genes-of-interest (GOI) into two ... on a single plasmid (Grefen and Blatt, 2012). Similar to traditional Gateway™ cloning, the GOI first ...the mitochondria, because cPYP binds to BoPD. A similar set of newly identified light- and dark- binder...

...organizations with similar ideas who were also thinking about reproducibility and shared similar desires to take...talented and motivated groups and individuals with similar ideas to actually do something about it. Building...

... a way to track successfully engineered cells. Similar to transgenic mice, knockout mice are engineered...off-target effects on regions of the genome with similar homology to the site of interest or create mosaic...recombination of floxed sites in the cell can occur. Similar systems include tetracycline or doxycycline mediated...

...same target to see if the results are the same/similar, emphasizing the antibody’s specificity. Strength...from the background noise. If the sample images similarly to the negative control, that informs us that ...doi.org/10.1038/518027a Freedman LP, Cockburn IM, Simcoe TS (2015) The Economics of Reproducibility in Preclinical...

...by daughter cells during cellular reproduction, similar to the host’s chromosome, or they can spread between...plasmids likely have undiscovered functions, many are simply genetic parasites. Cryptic plasmids tend to be ... R., Sailland, A., Auriol, P., Vogel, T. M., & Simonet, P. (2001). Laboratory-scale evidence for lightning-mediated...