We narrowed to 193 results for: SPL

1. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

   Type

   Blog Post

   Published

   Dec. 13, 2018, 1:14 p.m.

   ...sucrose or other osmolytes to induce hypertonicity display enhanced monoclonal antibody production (Oyaas,...

2. Summer SciComm Series: Master of SciComm

   Type

   Blog Post

   Published

   July 21, 2022, 1:15 p.m.

   ...illustrate the breadth of knowledge and passion on display in our building, each day brushing past and chatting...

3. Hot Plasmids and Viral Preps - September 2021

   Type

   Blog Post

   Published

   Sept. 21, 2021, 1:15 p.m.

   ...disrupt gene function by targeting start codons, splice acceptor and donor sites, and introducing premature...

4. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

   Type

   Blog Post

   Published

   Sept. 14, 2021, 1:15 p.m.

   ... it recombines at the 5’ and 3’ LTR regions, it splits into two smaller plasmids. The ~3.7 kb recombined...

5. Viral Vectors 101: What is a Virus (and a Viral Vector)?

   Type

   Blog Post

   Published

   Aug. 18, 2023, 4:15 p.m.

   ...replication process whereby one cell copies itself and “splits” into two cells. Viral vectors Viruses are quite...

6. What the HEK?

   Type

   Blog Post

   Published

   April 18, 2023, 1:15 p.m.

   ... fibroblast. However, Frank Graham showed HEKs display many immature neuron-like qualities, which is inconsistent...

7. Hot Plasmids: FRET-Based Biosensors

   Type

   Blog Post

   Published

   May 6, 2014, 1:07 p.m.

   ... microscope using commercially available beam splitters. In addition to imaging neurons, Griesbeck adds...

8. Learning Technical Skills at a Lab Central Workshop

   Type

   Blog Post

   Published

   Feb. 20, 2018, 2:30 p.m.

   ...presentation describing the technology. Then we split into pairs to actually try out the kit itself. This...

9. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

   Type

   Blog Post

   Published

   Dec. 10, 2019, 1:58 p.m.

   ...amenable to use with other bEBP/UAS pairs, and displays high dynamic range. However, the promoter in question...

10. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

    Type

    Blog Post

    Published

    July 27, 2017, 1:11 p.m.

    ...production of both types of HMO biosensors and displayed her findings at the Spring 2017 ACS meeting. She...

11. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

    Type

    Blog Post

    Published

    July 21, 2017, 12:48 p.m.

    ...PMID: 28704652. 6. V. Rodríguez, D. Didiano, C. Desplan, Power tools for gene expression and clonal analysis...

12. The Power Behind NGS Plasmid Validation: seqWell

    Type

    Blog Post

    Published

    April 19, 2017, 3:25 p.m.

    ...because the sample identity information is always displayed at the individual read level, and because custom...

13. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type

    Blog Post

    Published

    Nov. 5, 2019, 1:28 p.m.

    ...David Liu Lab similarly described xCas9, which displayed the ability to target multiple PAM sequences (...

14. Targeted Mutagenesis with EvolvR

    Type

    Blog Post

    Published

    Feb. 21, 2019, 1:15 p.m.

    ...its native endonuclease activity degrades the displaced strand.   Figure 1: EvolvR consists of ...

15. Luminescent Imaging with Nano-lanterns

    Type

    Blog Post

    Published

    May 25, 2017, 2:30 p.m.

    ...very robust, with an eNL-based calcium sensor displaying a 500% signal change upon calcium binding. Suzuki...

16. Screening for Successful Genome Editing with Digital PCR

    Type

    Blog Post

    Published

    March 30, 2017, 2:30 p.m.

    ...below. Digital PCR Digital PCR (dPCR) involves splitting up a sample into thousands of physically isolated...

17. Hot Plasmids - May 2022

    Type

    Blog Post

    Published

    May 26, 2022, 1:15 p.m.

    ...work, they have found at least two variants that display increased neuronal expression in the brain while...

18. Antibodies 101: ChIP

    Type

    Blog Post

    Published

    March 10, 2022, 3:25 p.m.

    ...choose, you’ll need to reserve a small aliquot, split into two parts to (1) run a DNA gel to confirm fragment...

19. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type

    Blog Post

    Published

    Feb. 10, 2016, 3:30 p.m.

    ...is also common. Overlap extension PCR is used to splice together two PCR amplicons based on sequence homology...

20. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type

    Blog Post

    Published

    March 19, 2019, 1:31 p.m.

    ...the Uppalapati and Woolley lab developed a phage display technique using small scaffold proteins to identify...