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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 351 results for: SUA

Showing: 121 - 140 of 351 results

  1. PhD Applications After COVID

    Type
    Blog Post
    Published
    Jan. 5, 2023, 2:15 p.m.
    ...the first steps in the application process and usually occurs in the few months leading up to applications...such-and-such university State how you found their lab (usually through the university website or, even better,...

  2. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...protein of interest is untagged and functions per the usual. In the presence of a specific protease inhibitor...developed by Promega as an affinity tag for visualization of proteins of interest. A fluorescent Halo ...

  3. Hot Plasmids: Summer 2025

    Type
    Blog Post
    Published
    Aug. 28, 2025, 1:15 p.m.
    ...characterized and deposited a variety of constructs for visualizing cellular components (nucleus, endosomes, lysosome...and an epitope tag (ALFA-tag, or At) for easy visualization. A cleavage site for endogenous BACE1 protease...

  4. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ...are smaller antibodies.They are derived from an unusual type of IgG antibody called a heavy chain antibody...fluorophore conjugates are used, but these are usually more expensive than unconjugated primary antibodies...

  5. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    Published
    Aug. 30, 2022, 1:15 p.m.
    ...immunofluorescence (IF) - a common method for visualizing molecules of interest within a cell or tissue...your sample type. Starting with a protocol that usually works in your system and then optimizing from there...

  6. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    Published
    Feb. 6, 2020, 3 p.m.
    ...responsible for each figure. Others still are more casual with a single presenter summarizing the paper’s...could be solicited via multiple avenues including casual request via email, in person, or instant messaging...

  7. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ...Selecting for seeds that have the tDNA insertions (usually via seed growth on antibiotic-containing media)...from the Dinneny lab that additionally allow for visual selection of transformants based on expression ...

  8. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    Published
    Sept. 3, 2015, 2:30 p.m.
    ... into a completely new realm. It allows for the usual office dynamics to be mixed up a bit. Who know that...word of mouth - be sure to bring up your event in casual conversation to get people excited!    Laura ...

  9. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    ... Protein tags are usually smallish peptides incorporated into a translated protein. As depicted in the... purified using metal-affinity chromatography, usually using a Ni2+ column. Like other affinity tags, ...

  11. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    Published
    Nov. 21, 2024, 10:47 p.m.
    ...launched the Addgene YouTube channel to add a strong visual component to our educational resources. From 2013...a plasmid and its parts that helps the viewer visualize something that cannot be seen with the naked eye...

  12. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    Published
    May 21, 2024, 1:30 p.m.
    ... where cover letters are common, the answer is usually yes! A good cover letter won’t hurt your application... matches the job requirements, and address any unusual circumstances or proactively answer questions the...

  13. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    Published
    March 31, 2020, 1:15 p.m.
    ...cases where one plasmid outcompetes the other, usually a smaller, higher copy plasmid can more effectively... that lead to asymmetrical plasmid loss of the usually large, low copy virulence plasmids. This strategy...

  14. How to Be an Excellent Trainee

    Type
    Blog Post
    Published
    Sept. 26, 2023, 1:15 p.m.
    ...bench mentor is also the scientific mentor…but usually the bench mentor is a staff scientist, postdoc,...the next day. In the latter case, you can say “I usually need time to gather my thoughts, but always have...

  15. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    Published
    July 13, 2023, 1:15 p.m.
    ...request a DNA pooled library from Addgene, you’ll usually receive an aliquot from the original library constructed...representative NGS results (read counts or another visualization) that confirm diversity of the library sample...

  17. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ...Pinterest is also a favorite for millions and their visual format is quite appealing to many audiences. While... of these platforms (such as social networking, visual appeal, instant sharing across multiple other sites...

  18. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    Published
    Oct. 13, 2016, 2:30 p.m.
    ...imminently. Consulting firms deploy case teams, usually with 3-6 consultants, who work on analyzing the...consulting meant for PhDs? Healthcare consultants are usually trained in the life sciences, in business, in healthcare...

  19. Five Popular Model Organisms

    Type
    Blog Post
    Published
    April 11, 2019, 12:39 p.m.
    ...protein or disruption of a gene, can be easily visualized in a living organism from embryonic development...adulthood. This ability to genetically manipulate and visualize cells and tissues allows scientists to conduct...
Showing: 121 - 140 of 351 results