... of the environment of the department, the lab setting, and how students truly feel about the program ...stressful time, and I was more relaxed during both sets of interviews. I also had universities change their...

...one set of genetic loci can be introduced together with truncated gRNAs targeting a different set of loci...

...proposing new programs, we're always striving to set an example of environmental stewardship.  Additional...

...have skill in a particular technique? Do you have a set of tips you give to every new lab mate? Do you have...

... and their team at Calico Life Sciences recently set out to do just that — by developing a new fluorescent...response. Evolution of a new variant Maria and the team set up a directed evolution and screening experiment ...

...that can be difficult to replicate in other work settings: The sense of being the first to discover something...academia provide a formal training regime and the right set of tools to help you do your job as well as possible... find the idea of not holding a pipette again upsetting, there are ways to get back to the bench during...

...variety of transferable skills. At Addgene we've set out to fill this gap by both highlighting that researchers...!). Then schedule a meeting to discuss the work, set expectations, answer any questions, and (most importantly...

...variety of transferable skills. At Addgene we've set out to fill this gap by both highlighting that researchers...doesn’t end after you get your first new job. To set yourself up for success in the career you hope to...

...way to develop and exercise your scientist skill set. If you dread the thought of writing a review, or...I was by no means an expert on this topic when I set out to write the review. That’s just to say that ... isn’t possible. So, define your scope from the onset. Perhaps you, a colleague, or your adviser was invited...

...cloning technique that will work well with a given set of resources, time, and experimental needs. Since...restriction and ligation cloning. It is also possible to set up the BP and LR reactions in the same tube, speeding... create an entry clone: Restriction cloning the inset into an attL-containing entry vector.   Pro...

...communicating ideas in a content-rich, easy-to-understand set of PowerPoint slides. What are some of the more challenging... biology or medicine you will bring an essential set of tools to a consulting team. Your case team will...

...will need to grow this initial stock and create a set of vials of ‘Master’ stock, which can be stored in...Master vial can then be thawed and used to produce a set of ‘working’ stocks of cells. Working stocks should...

...order to try and access the entire interactome of a set of proteins at once.  However, as Y2H screens are...positive and false negatives, these experiments must be set up, conducted and analysed with care to get reliable...entire proteome of a particular cell type, or a subset based on a cellular compartment or growth stage... as a good positive control to ensure that your setup works. The last thing you want is to run your lovely...

...plenty of events that included a simple corn hole set and a cooler. Chances are one of your colleagues ...especially true. If your event requires a lot of set up or cleanup, ask for volunteers to help out. Delegate...

...make sense of it, with their decision to publish setting the clock for others to dive in. The real cost ...usually condensed into a statistically evaluated set of values.  However, to evaluate the interpretation...anonymous request.  This means that supporting datasets must be logically annotated and arranged, as in...should be primarily responsible for storage.  If datasets are too large to transfer to a journal repository...peer-reviewed repository for “scientifically valuable” datasets. It likely won’t be long before other data repositories... move towards encouraging authors to integrate datasets with their resultant figures. Instead of providing...

...Blog Series We have not one but two new blog series set to launch this summer! Summer SciComm Series From...

...scavenger hunts. In fact, they’ve been following a set of clues that has led them to their biggest discovery...

...hoarding used tip boxes for a lab supply tree and set a challenge to my lab mates. They did not disappoint...

...is an integral part of any bacteriologist’s skill set. So when Dave Westenberg taught this concept in his...the streak plate challenge. Chromoproteins are a subset of the fluorescent protein family that give off...

... super family). To overcome these challenges, we set out to develop a hassle-free, highly efficient Cas9...eLife 2018;7:e35069 DOI: 10.7554/eLife.35069. How to set up csRNP knock-in experiments If your lab is new ...experiments, there is no need to worry at all. Setting up tag knock-in experiments using the csRNP method...