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TypeBlog PostPublishedNov. 29, 2016, 3:30 p.m.... spend a few days editing and later looking for songs and sounds to synchronize with the images thereby...the hardest part of the process was to embed personality in pieces of plastic, but it’s gotten easier ...than a piece of plastic for us now). With the personality in place, everything now is about the plot. This...How You Can Use Video to Share Your Research 5 Reasons to Use Reddit for Science Communication 10 Ways...
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Management for Scientists: Giving Feedback
TypeBlog PostPublishedFeb. 6, 2015, 2:46 p.m....to improve, and they’re also clear about how the person is doing overall. Employees hate to wonder what...employee. Make sure to focus on the action, not the person - intent is usually good. Don’t pretend something...standard format not only enables you to discuss a person's performance or what has been learned, it helps... effective over time if you take it seriously. Person who will receive feedback does a self-assessment... -
Choosing a Good Mentor for Scientists
TypeBlog PostPublishedJan. 16, 2014, 2:56 p.m.... ask a lot of questions. Personality and style conflicts are a common reason for scientists to change ...budgeting, equipment acquisition, public speaking and personnel management? If you are thinking about a non-academic... you can help it. Observe as much as you can in person. Rotate in the lab, if possible. Attend a lab meeting... -
Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring
TypeBlog PostPublishedNov. 12, 2019, 2 p.m....process samples at the picnic table before dark, a bison wandered into our campsite. We had to abandon our...our samples and move farther away until the bison meandered off. In the end we were able to safely get...get back to our lab work. Figure 3: When a bison wanders into the campsite, leave your samples behind...ThermoCas9 from the van der Oost lab. Phylogenetic comparison indicates that like GeoCas9 and ThermoCas9, IgnaviCas9... -
The Strength of Story Telling
TypeBlog PostPublishedDec. 19, 2017, 2 p.m....end; cause, effect; lesson, learning. Storytelling is a crucial part of interpersonal communication, which... it came time to add author details a different person in the lab wanted to be the first author. When ...protagonist will be you. Make sure to highlight what lessons you’ve learned and what knowledge you’ve gained... -
What Do I Do Now? Academic v. Non-Academic Career Decisions
TypeBlog PostPublishedMarch 9, 2017, 3:40 p.m.... In fact, there are a lot of good reasons to stay and good reasons to go. Here are some questions and...especially race and gender) Interpersonal issues can create interpersonal issues (one bad apple can change... -
Another Pathway into Cells: iTOP
TypeBlog PostPublishedJune 23, 2015, 8:37 p.m....Although CRISPR/Cas9 is all the rage, they had another reason to test this system. It’s difficult to determine...require multiple transduction events. For these reasons, iTOP is best suited to binary systems in which... size associated with viral vectors. For these reasons, iTOP, especially in tandem with genome editing... VP22 nuclear homing an artifact? Lundberg M, Johansson M. Nat Biotechnol. 2001 Aug;19(8):713-4. PubMed... -
Antibody Validation for Flow Cytometry
TypeBlog PostPublishedSept. 10, 2024, 1:15 p.m.... T. M., Edwards, A. M., Healy, L. M., Robertson, J., & McPherson, P. S. (2019). Implementation of an antibody...perform as well in other conditions. For these reasons, it is essential to test the selectivity of your...cannot prove antibody selectivity, and for that reason, it is best practice to combine correlation with...C., Bandrowski, A., Virk, H., Edwards, A. M., McPherson, P., & Laflamme, C. (2023). Scaling of an antibody... -
The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs
TypeBlog PostPublishedNov. 3, 2015, 3:30 p.m....Thanks to our guest blogger Cameron MacPherson! Cameron R. MacPherson is Lead Data Scientist for the Milieu...post was contributed by guest blogger Cameron MacPherson at the Institut Pasteur CRISPR software and the... by their focus on specific organisms. For this reason I generally recommend that laboratories that have...CRISPR technology to a new organism: For obvious reasons you can rule out any tool that limits you to a ... -
Using CRISPR/Cas9 to Edit Disease Out of the Genome
TypeBlog PostPublishedJune 23, 2022, 1:15 p.m.... by Kendall Morgan and updated in 2022 by Lucie Wilson. Lucie is an Addgene co-op from Northeastern University...in monoallelic diseases In 2013, a team led by Jinsong Li from the Chinese Academy of Sciences found that...fibrosis (8). The difference with CRISPR/Cas9 in comparison to other genome editing techniques is its ease...s Disease.” Journal of Alzheimer's disease & Parkinsonism. https://doi.org/10.4172/2161-0460.1000439 6... -
K. phaffii: Rising to the Occasion
TypeBlog PostPublishedApril 4, 2023, 1 p.m....make for an excellent model organism for a few reasons: Affordable to culture and easy to work with ...established in yeast, including a widely used Parkinson’s experimental model. In fact, approximately 25%...of the uses outlined below. Biology They say comparison is the thief of joy, but I think it’s acceptable...aren’t going anywhere anytime soon, and for good reason! However, there are other players in the game (... -
Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors
TypeBlog PostPublishedJuly 27, 2017, 1:11 p.m....fluorescent proteins everyday. The late Michael Davidson and Roger Tsien played enormous roles in enabling...their legacy, we recently launched the Michael Davidson and Roger Tsien Commemorative Travel Awards. These... we’re proud to announce our first two Michael Davidson and Roger Tsien Commemorative Travel Awardees:...Plasmids from the Tsien Lab Browse the Michael Davidson Collection ... -
Addgene Depositors Get More Citations
TypeBlog PostPublishedApril 20, 2017, 2:30 p.m....contributed by guest bloggers Samantha Zyontz and Neil Thompson from the MIT Sloan School of Management. Professor...depositing a plasmid with Addgene, in contrast to other reasons for the correlation. However, determining this ...to our guest bloggers, Samantha Zyontz and Neil Thompson. Special thanks to Addgenies Pamela McA'Nulty ...page: https://ssrn.com/author=1232466. Neil Thompson is an Assistant Professor of Innovation and Strategy... -
Four Factors that Differentiate the Stem Cell Field
TypeBlog PostPublishedSept. 28, 2016, 2:35 p.m....When our Director of Biology, Lianna Swanson, suggested that I might be able to attend the 10 Years of...degeneration; Laurenz Studer discussed his work with Parkinson's Disease; Hideyuki Okano presented ground-breaking...clinical trials for ALS. I have an uncle with Parkinson's, a half-brother who is a quadriplegic due to ... are key to the research, but they are also the reason this research is happening. Stem cells scientists... -
Synthetic Photobiology: Optogenetics for E. coli
TypeBlog PostPublishedSept. 8, 2015, 2:30 p.m....Levskaya A, Chevalier AA, Tabor JJ, Simpson ZB, Lavery LA, Levy M, Davidson EA, Scouras A, Ellington AD, Marcotte...Chemical effectors are suboptimal for a number of reasons, including their potential toxicity and cross-reactivity...genetic edge detection program. Tabor JJ, Salis HM, Simpson ZB, Chevalier AA, Levskaya A, Marcotte EM, Voigt... -
Management for Scientists: Delegating is Key
TypeBlog PostPublishedFeb. 20, 2015, 8:30 p.m....come into being through the actions of a single person but through the coordinated efforts of many individuals...collection page, we certainly don't expect that one person will write all the copy, design every icon, curate...page. That would be entirely too much work for one person, it would take forever, and the end product would...who can do the job. It is good to delegate so the person doing the task will have to learn and stretch a... -
Sweating the Small Stuff: Details in the Lab
TypeBlog PostPublishedFeb. 8, 2022, 2:15 p.m....in the form of a protocol, and you can add in personalized steps like “check drawers for light-sensitive.... References and Resources Images used Roberson Lab, UPenn. Western blotting. Retrieved Feb. 7... 7, 2022. https://www.med.upenn.edu/robertsonlab/assets/user-content/documents/Western%20Blotting.pdf ... Rachel Leeson... -
Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number
TypeBlog PostPublishedSept. 4, 2018, 12:53 p.m....PMID: 29553576. 2. Kittleson, Joshua T., Sherine Cheung, and JChristopher Anderson. "Rapid optimization..., and ColE2 plasmids) as cell division slows (Kittleson et al., 2011). Segall-Shapiro et al. showed that...References 1. Segall-Shapiro, Thomas H., Eduardo D. Sontag, and Christopher A. Voigt. "Engineered promoters... -
Making CRISPR Plasmids Using Fragmid
TypeBlog PostPublishedAug. 29, 2024, 1:15 p.m....AAV pRDA_889 (ITRs from AAV2) Mammalian Transposon pRDA_757 (Piggybac; constitutive expression... drosophila) Delivery type (lentivirus, AAV, transposon, Plasmid) Destination vector (dropdown options..., Bosch, J. A., Zirin, J. D., Viswanatha, R., Sontheimer, E. J., Goodale, A., Greene, M. A., Green, T.... Rachel Leeson... -
A Guide to Designing a Scientific Poster: Content, Layout, and More
TypeBlog PostPublishedOct. 22, 2019, 12:20 p.m....also probably thought about why certain posters resonate with you (or not). It is important to reflect ...length, and will be more difficult to read. The Thompson Rivers University Library provides useful tips...scientific poster design was conceived by Mike Morrison, a psychology doctoral student at Michigan State... avoiding red and green is a good idea for this reason but there are many other ways to make your poster...