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We narrowed to 542 results for: TIL

Showing: 121 - 140 of 542 results

  1. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    Published
    Nov. 5, 2014, 3:41 p.m.
    ...the way we apprehend molecular complexes and is still a very useful tool.    Like a guitar hero (that ...SYFP2A, mTurquoise/mVenus and others. CFP-YFP is still one of the best and most used pairs to measure FRET... faster measurement systems, this technique has still many good days and good “gigs” ahead...No offense...

  2. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    Published
    June 17, 2025, 1:15 p.m.
    ...something that has fallen out of fashion but is still useful and most importantly, effective. In the research...valuable tool for your research arsenal, and there is still a dedicated field of study for improving these tools... (2009). The RNA-induced Silencing Complex: a versatile gene-silencing machine. Journal of Biological ...

  3. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    Published
    June 27, 2024, 1:15 p.m.
    ...can be achieved through two-protein labeling by utilizing(a) biotin-streptavidin binding and (b) antibody...are protected. Amine-binding viability dyes can still bind free amines on the cell surface, which yields...As a result, the largely negative live cells can still be easily gated on during the analysis. Conclusion...

  4. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    Published
    Feb. 22, 2017, 3:30 p.m.
    ...mutant, this technique uses mutagenic primers that tile across a region of interest, generating multiple...) at a given codon site and multiple primer sets tile across the protein or region of interest. A low ...). If so, the nicking mutagenesis protocol will still work if the orientation of the BbvCI site(s) of ...

  5. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    Published
    Sept. 29, 2020, 1:15 p.m.
    ...Escherichia coli One of the most recognizable and utilized model organisms is Escherichia coli. The bacterium... highly characterized genome, and widespread utilization, Arabidopsis has made significant contributions...number of offspring (produces many seeds), self fertilizing, a small yet highly characterized genome, and...

  6. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    Published
    Nov. 25, 2015, 4 p.m.
    ...track position 5 years after receiving their PhDs, still strive to become academic researchers themselves...universities to hire a glut of trainees at low wages will still be there without restructuring. Positive Points ...highlighting some of the important questions that still need to be answered about the makeup and welfare...

  7. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    Published
    Nov. 7, 2014, 2:56 p.m.
    ...F Host does (F') or does not (F-) contain the fertility plasmid. A low copy-number plasmid, encodes proteins...hsdR(rk-, mk+) Unmethylated DNA not degraded, cell still can methylate DNA   hsdS(rk-,mk-) Unmethylated...disruption of arabinose metabolism pathway Inability to utilize arabinose as a carbon source leuB β-isopropyl...

  8. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    Published
    May 23, 2023, 1:15 p.m.
    ...fluorophores, then labeling with the other, you can still record the signal separately for each target.   ...., 2020 multiplexed over 65 targets!) IF is a versatile method with endless possibilities, and there are...Hernandez, J. M., & Germain, R. N. (2020). IBEX: A versatile multiplex optical imaging approach for deep phenotyping...

  9. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    Published
    Aug. 5, 2025, 1:15 p.m.
    ... ways to engineer an antibody, thanks to the versatility of the basic antibody structure (Figure 1). Antibodies...’ll typically go through this cycle many times, until you achieve whatever goal you’re aiming for. When...development methods. Despite recent advances, there is still always room to improve, and scientists will continue...

  10. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    Published
    March 7, 2023, 2:15 p.m.
    ...normality (or lack thereof). Alternatively, a Quantile vs. Quantile plot can determine if your data deviates...data in ascending numerical value relative to quantiles derived from a theoretical distribution. If your...

  11. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    Published
    Oct. 24, 2017, 1:54 p.m.
    ...myths that abortions cause breast cancer and infertility or to argue against unnecessary clinic regulations...had been so well-publicized, we might encounter hostile anti-science activists. However, I must say that...in Vancouver, BC, although I wasn’t sure I’d go until several hours before it started. It was great to...

  12. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ... the wrong address in a different city, you can still develop a successful FRET-based assay since energy... Scientific). These reagents are nonfluorescent until they bind a 6-residue tetracysteine tag (sequence...that can be inserted into the target protein. The utility of these reagents is that binding is covalent, ...

  13. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    Published
    Sept. 28, 2020, noon
    ... is done with recombineering, a technique that utilizes phage recombination machinery to promote homologous...targeted and preferentially depleted from gut. While still far from the clinic, CRISPR-Cas3 systems have been... genus. Work from the Sun lab demonstrates the utility of cytidine and adenine base editors that enable...

  14. Our New Antibody Mascot is....

    Type
    Blog Post
    Published
    Feb. 24, 2022, 2:15 p.m.
    ...vote on @addgene's Twitter poll. Voting is open until Feb. 22, 2022, and we'll unveil the winning name...if it’s also a great pun.) Submissions are open until Feb. 3, 2022.  The Addgenies will choose up to four...

  15. Streaking for Single Colonies: The Streak Plate Challenge

    Type
    Blog Post
    Published
    March 2, 2021, 2:15 p.m.
    ...pic.twitter.com/mk44J4Gcj9 — Dave W will get vaccinated and still wear a mask. (@GermJuggler) February 13, 2021 Westenberg...over the second quadrant. The process continues until all quadrants are used, hopefully revealing single...

  16. Top Requested AAV of 2017: pmSyn1-EBFP-CRE

    Type
    Blog Post
    Published
    Jan. 17, 2018, 2:57 p.m.
    ...our viral service in late 2016 and requests are still pouring in! While our lentiviral service is going... they come from and what do they mean? AAV: A Versatile Tool for Gene Expression in Mammals Subscribe ...

  17. What's New in CRISPR - December 2019

    Type
    Blog Post
    Published
    Dec. 17, 2019, 2:37 p.m.
    ...new CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools...of ADAR2 into a cytidine deaminase. This editor still retains A-to-I editing. This system is called RNA...

  19. Six Spooky Science Stories and Halloween at Addgene

    Type
    Blog Post
    Published
    Oct. 31, 2019, 1:06 p.m.
    ... leave their foraging trail. They climb upwards until they reach a point where the temperature and humidity...enters fish through its gills. It attaches there until they mature and changes sex from male to female....

  20. Searchable and Sortable gRNAs for Your Next CRISPR Experiment

    Type
    Blog Post
    Published
    May 17, 2016, 2:30 p.m.
    ...This post was written jointly by Addgenies Brook Pyhtila and Nicole Waxmonsky Resource sharing shortens ...grow. This post was written jointly by Brook Pyhtila and Nicole Waxmonsky. References McDade J, Waxmonsky...
Showing: 121 - 140 of 542 results