...can be achieved through two-protein labeling by utilizing(a) biotin-streptavidin binding and (b) antibody...are protected. Amine-binding viability dyes can still bind free amines on the cell surface, which yields...As a result, the largely negative live cells can still be easily gated on during the analysis. Conclusion...

...track position 5 years after receiving their PhDs, still strive to become academic researchers themselves...universities to hire a glut of trainees at low wages will still be there without restructuring. Positive Points ...highlighting some of the important questions that still need to be answered about the makeup and welfare...

...F Host does (F') or does not (F-) contain the fertility plasmid. A low copy-number plasmid, encodes proteins...hsdR(rk-, mk+) Unmethylated DNA not degraded, cell still can methylate DNA   hsdS(rk-,mk-) Unmethylated...disruption of arabinose metabolism pathway Inability to utilize arabinose as a carbon source leuB β-isopropyl...

...fluorophores, then labeling with the other, you can still record the signal separately for each target.   ...., 2020 multiplexed over 65 targets!) IF is a versatile method with endless possibilities, and there are...Hernandez, J. M., & Germain, R. N. (2020). IBEX: A versatile multiplex optical imaging approach for deep phenotyping...

...normality (or lack thereof). Alternatively, a Quantile vs. Quantile plot can determine if your data deviates...data in ascending numerical value relative to quantiles derived from a theoretical distribution. If your...

...myths that abortions cause breast cancer and infertility or to argue against unnecessary clinic regulations...had been so well-publicized, we might encounter hostile anti-science activists. However, I must say that...in Vancouver, BC, although I wasn’t sure I’d go until several hours before it started. It was great to...

... the wrong address in a different city, you can still develop a successful FRET-based assay since energy... Scientific). These reagents are nonfluorescent until they bind a 6-residue tetracysteine tag (sequence...that can be inserted into the target protein. The utility of these reagents is that binding is covalent, ...

... is done with recombineering, a technique that utilizes phage recombination machinery to promote homologous...targeted and preferentially depleted from gut. While still far from the clinic, CRISPR-Cas3 systems have been... genus. Work from the Sun lab demonstrates the utility of cytidine and adenine base editors that enable...

...vote on @addgene's Twitter poll. Voting is open until Feb. 22, 2022, and we'll unveil the winning name...if it’s also a great pun.) Submissions are open until Feb. 3, 2022.  The Addgenies will choose up to four...

...pic.twitter.com/mk44J4Gcj9 — Dave W will get vaccinated and still wear a mask. (@GermJuggler) February 13, 2021 Westenberg...over the second quadrant. The process continues until all quadrants are used, hopefully revealing single...

...our viral service in late 2016 and requests are still pouring in! While our lentiviral service is going... they come from and what do they mean? AAV: A Versatile Tool for Gene Expression in Mammals Subscribe ...

...new CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools...of ADAR2 into a cytidine deaminase. This editor still retains A-to-I editing. This system is called RNA...

...phages to the directory does not typically come out until nearly the last minute when all other options have...We don’t want to cry wolf to the phage community until we have someone that really needs it, but then it...

... leave their foraging trail. They climb upwards until they reach a point where the temperature and humidity...enters fish through its gills. It attaches there until they mature and changes sex from male to female....

...This post was written jointly by Addgenies Brook Pyhtila and Nicole Waxmonsky Resource sharing shortens ...grow. This post was written jointly by Brook Pyhtila and Nicole Waxmonsky. References McDade J, Waxmonsky...

...seeking a first job after the academic bench. Utilizing existing relationships as part of the job application...you a much better chance at an interview –but you still need to nail the job yourself.   See the links in...

...been there to answer your plasmid questions. But until now, you’ve always had to call the United States...and depositing (all while our US counterparts are still sleeping). Plasmid sharing in Europe: Addgene’s ...

...and transformation. While restriction cloning is still routinely performed by many labs, a variety of other... of cloning has its advantages, most scientists still encounter several struggles with these techniques...

...response to tissue damage, have excessive bone growth until eventually the patient loses mobility. Attempts ...novel solutions to treat rare diseases like FOP until a cure is available. Addgene and AJ's Avengers This...

...curriculum development team at eScience Labs. She still gets to write curriculum, but has taken on other...responsibilities.“I’d say maybe 25% of my day is still what I did as an SME, and then 75% of the time I...