We narrowed to 635 results for: UTY

...enhanced function after intravenous administration, distributing widely throughout the organism to target large.../cJ mice through LY6C1, with relatively even distribution of target gene expression (EGFP, in the depositing...transduction in New World monkeys   Figure 3: Evolutionary tree depicting the phylogenetic relationship...efficiency across species points to its potential utility in translational applications and in models such... written by Rachel Leeson, with significant contributions from Cynthia Arokiaraj. Addgene would also like...epithelium after intravenous delivery by directed evolution of underexplored sites on the AAV capsid. Molecular...Administration Results in a Differential Pattern of CNS Biodistribution in Non-human Primates Compared with Mice. Molecular...

...seemingly ubiquitous in nature, they are largely underutilized in research settings — mostly a consequence ...Cas3 — a tactic some CRISPR transposase systems utilize with type I-F Cascades. Figure 2: Cascade...has the potential to be incredibly useful in therapeutics. Imagine deleting a problematic copy of a gene... potential. Hopefully more people will want to utilize Cas3’s degradative power in the future! References..., J., . . . Koonin, E. V. (2015). An updated evolutionary classification of CRISPR–Cas systems. Nature...

...models for developmental processes and promising therapeutic tools. The two unique characteristics that define...cells can be used for different research and therapeutic purposes. Both types have their advantages and...of post-transplantation rejection when used therapeutically High Low (if ASCs are isolated from the patient...  While iPSCs and ESCs have similar functional utility, iPSCs bypass the transplantation compatibility...directly from a patient’s adult cells when used therapeutically, the chances of post-transplantation rejection...

...and Henry Siedentopf to enhance the microscopic resolution for studying colloidal gold (1).  The method ... microscope (SPIM). In order to improve axial resolution and reduce phototoxicity for imaging live specimens...using multi-view imaging can provide enhanced resolution (8,9).  Thus, LSFM is ideal for 3D reconstruction...was done by Walter Spalteholtz (10).  He used a solution of benzyl alcohol and methyl salicylate which ...later modified by others to produce Murray’s clear solution.  Murray's clear (11) was mostly used to study...

...with limited time for one-on-one mentoring. His solution? Bringing his research into the classroom. I had..., Provost partnered with colleagues at other institutions to form a support network called the MDH CUREs...MDH-focused CUREs at 50 schools, ranging from R1 institutions to community colleges, and has helped provide... and results with peers and faculty at other institutions. Provost believes these networking experiences...experiences (CUREs) as a pathway to diversify science. Evolution and Development. 24(5):127-130. Kimball, C., Powers...

...typically carried out in one of two ways. Both methods utilize the same basic strategy: immobilize the antibody... One of the most common immobilization methods utilizes bacterial cell wall proteins, Protein A and Protein... be performed even with a pre-cleared sample.  Eluting the target protein for downstream testing Once ...washing, the target protein can be eluted. The elution method will vary depending on the specific target...with nucleic acids. Labs studying epigenetics routinely use the chromatin IP (ChIP) technique to identify...

... ways. Zebrafish mutants In the 1990’s, two revolutionary large-scale random mutagenesis screens produced...processes—not just development—are available from strain distribution centers in the US, Europe, and China. In addition...microscopy-based lineage tracing defined a high-resolution map of early embryogenesis in time and space,...fish lines have been developed to enable high-resolution mapping of the brain by uniquely labeling neurons.... "Pan-neuronal calcium imaging with cellular resolution in freely swimming zebrafish." Nature methods...

...are investigating how the production of cancer therapeutic drugs, vinblastine and vincristine, are regulated...transferred into the plant. In the laboratory we can utilize this natural process by replacing the genes on ...easily show which tissue have been transformed. But caution: check for GUS-like activity of your tissue. Run...or whole plants are placed in an Agrobacterium solution and a vacuum is applied with a vacuum bell or ...the dark 16 hours before the transformation. We routinely transfer our plants 16 hours before the transformation...

...commercialization office, the CIO’s office, and research computing. The two guiding principles emerging from the ...Goettingen, and several other large research institutions. The following graphic depicts the ways in which...banks and sequencing files is likely stored in institutional and lab servers specifically designed to hold...illustrated in this video, and to data stored on institutional and lab servers. Connectivity to other tools...well as the DSpace repository. These integrations utilize an interface that enables users to conveniently...

... contaminated or misidentified cell lines. The solution Fortunately it has never been easier to conduct...working cell bank has been made by a research institution (an excellent practice), or maybe cells have ...major cell lines on public databases. Image attributions (from top to bottom) Database Center for Life...experience through working for a variety of pharmaceutical companies including GSK, AstraZeneca-MedImmune...talks and seminars on cell culture and other pharmaceutical topics. The LGC Group also provides standards...

...be precise, the fixing solution is different while the permeabilization solution can be the same). Examples...can be achieved through two-protein labeling by utilizing(a) biotin-streptavidin binding and (b) antibody...then permeabilized, using two different buffer solutions. Fixing causes protein cross-linking, killing ...species reactivity. When sorting cells with a high resolution for specific downstream applications, while maintaining...

... Returning to an earlier analogy, no amount of shouting will make an apple fall up. As scientist and politician...GMOs), the absence of a link between vaccines and autism, and other hypotheses mount yet policies and public...proposing to defund aspects of research at National Aeronautics and Space Agency, the Environmental Protection...Transgender issues Reproductive health Immigration Pollution Healthcare Science education To make scientists...and inclusion through funding and at their own institutions, while many also seek to honor and credit women...

...resultant gene targeting system could be used to routinely deliver sequences of interest to cells with efficiencies...CRISPR editing CRISPR, of course, has started a revolution in gene editing. However, while CRISPR is remarkably...the AAV approach is most well suited, since it routinely generates knockin efficiencies of 1-40% with G418...PMC4752446. 5. Asuri, Prashanth, et al. "Directed evolution of adeno-associated virus for enhanced gene delivery...nucleases with adeno-associated viral vectors for therapeutic gene editing." Precision Medicine, CRISPR, and...

...have waited. Both tasks have some utility, but I lost all the utility (fun) of eating with friends by splitting... two main things: How useful the task is (its utility) How much time it will take you to complete the...should err on the side of things that have the most utility; i.e. those things that will be most useful for...and think about what they mean in terms of the utility of your tasks. In my cell splitting anecdote, I...

...genome, and widespread utilization, Arabidopsis has made significant contributions to understanding plant...Escherichia coli One of the most recognizable and utilized model organisms is Escherichia coli. The bacterium...Iannaccone et al., 2009). Rodents have also been routinely used in an array of neuroscience research (Ellenbroek...Brown Norway rat yields insights into mammalian evolution. Nature 428, 493–521 (2004). https://doi.org/10.1038...

...2020). Directed evolution of adenine base editors with increased activity and therapeutic application. Nature...developed from RNA deaminases, using directed evolution to get them to work on DNA instead. However, they...to improve its effectiveness through directed evolution, introducing mutations to the deaminase subunit...Doudna, J. A., & Liu, D. R. (2020). Phage-assisted evolution of an adenine base editor with improved Cas domain...

...these issues, the Ting lab utilized yeast display-based directed evolution to generate two mutants, the...the Brian Burke Lab (Roux et al., 2012). BioID utilizes the E. coli biotin ligase, BirA*, with a site ...Thus they conducted multiple rounds of directed evolution until they identified a mutant with significantly...PMC4831873. Lam, Stephanie S., et al. "Directed evolution of APEX2 for electron microscopy and proximity...

...exist for this, the most common of which is the utilization of phosphomimetic residues. The phosphorylated...researchers with a valuable tool for understanding the contribution of phosphorylation to enzyme function. The development...tRNA that recognizes a specific ‘open’ codon and utilizes this triplet to incorporate phosphoserine A Sep-tRNA...events on its activation loop, but can be made constitutively active through phosphomimetic mutation of these...phosphoserine) to control for effects of the amino acid substitution. Alternatively, you can express the completely...

...clear on his science, and found a solution. However extreme a solution may seem, you should consider it...greater than $5,000 but this can depend on the institution/funding source. Major equipment includes large...your research training to help you find other solutions to reach your goals. For example, a junior investigator... point of a negotiation is to create a win-win solution for the success of your science. If the resources...

...of all our depositors here at Addgene — your contributions make the repository what it is. So we’re thrilled...strikingly similar to the design model at atomic resolution” (Kuhlman and Dantas et al., 2003). That made...folding and selective pressures during protein evolution. The Baker Lab has deposited multiple Top7 plasmids... at Addgene, along with other tools like high resolution Ras sensors and crosslinked peptides proposed...