We narrowed to 644 results for: Vars

...transpeptidase sortase A variant (mgSrtA), which is capable of promiscuously labeling various cell surface proteins...suitable for organoid or in vivo studies (Che and Khavari, 2017). Find APEX plasmids at Addgene! Since the...a small peptide (LPETG). In EXCELL, the mgSrtA variant can be displayed on the surface of a cell of interest...Central PMCID: PMC6126969. Che, Yonglu, and Paul A. Khavari. "Research Techniques Made Simple: Emerging Methods...

...by Jacob Lazarus, a postdoctoral researcher at Harvard. There’s an astounding number of ways to create...there is also a large amount of user-to-user variability, and in some cases, counter-selection escape ...thanks to our guest blogger Jacob Lazarus from Harvard. Jake received his MD and a PhD in Cell and Molecular...post-doctoral research in the lab of Matthew Waldor at Harvard. Now that he has an efficient allelic exchange ... PubMed Central PMCID: PMC3748948.  Khetrapal, Varnica, et al. "A set of powerful negative selection systems...

...across runs. If the results vary considerably, then the high degree of variability in the process could make...response recorded would be due to natural biological variation. This information is critical for correctly interpreting...of the same sample and are included to assess variability in the experimental protocol. In the experiment...assess naturally occurring random biological variability. If one were studying the effects of tau kinase...

... through the gel. For better protein separation, vary the acrylamide content of the gel. For small proteins...assessed from a single western.  There are several variations of the protein transfer step including wet, semi-dry...options targeting your protein of interest from a variety of vendors and you may even find plasmids encoding...Some vendors will also test their antibody in a variety of tissues to demonstrate that the protein staining...unintended cross-reactive proteins. Similarly, if you vary the experimental conditions from those used by the...

...Zafira Castano Corsino, a junior faculty member at Harvard Medical School/Brigham and Women’s Hospital (BWH...University Miguel de Cervantes, the University of Navarra, and the University of the Basque Country. Mentees...some of the top Universities in USA and Europe (Harvard University, Massachusetts Institute of Technology... Zafira Castaño Corsino is a Junior Faculty at Harvard Medical School/BWH, co-Director of the Mentor Circle...Fernandez-Godino is a Postdoctoral Researcher at MEEI-Harvard Medical School, where she studies age-related macular...

...allow you to optimize the concentrations of your various blot reagents. There are two types of gradients...antibodies, especially polyclonal antibodies, can vary lot-to-lot, this range needs to be empirically determined...antibodies, which have much lower lot-to-lot variability. If you’re running an already-developed assay...of protein in your samples. If you have a wide variety of protein amounts on the same blot, you may want...Add sonication step to protein lysis Splice variants Check literature Posttranslational modifications...use a less sensitive exposure kit No signal variation in highly expressed proteins Antibody dilutions...

...Ligases Small, varies Various Few Complicated Non-natural amino acids 1 Various Many Complicated...HaloTag is actually HaloTag7, and recent specialized variants run up to HaloTag11. HaloTag is now the most popular...any cloning at all! As mentioned above, several variants of these tags have been developed over the years...

... Amy Gilson from Science in the News (SITN) at Harvard University. There are tons of ways you can get ...podcast, the SITN team brings in scientists from a variety of fields as well as students from the ed school...Amy Gilson. Vini Mani is a graduate student at Harvard University, former Co-director of Science in the...     Amy Gilson recently received her PhD from Harvard University, is a former Co-director of Science ...

... sensor. This sensor was engineered from a GFP variant and a DA receptor such that GFP fluoresces when...when the receptor is bound by DA. Two variants of the sensor, dLight1.1 and dLight1.2, exhibit high (submicromolar...GRABDA sensor based on a similar strategy: a GFP variant bound to a DA receptor, such that the GFP fluoresces...detection and measurement of neurotransmitters in various species and while exhibiting complex behaviors....

...my PhD in Biological and Biomedical Sciences at Harvard University and entered a new role here at Addgene... to people. When I started my graduate work at Harvard, I recognized that I would ultimately want to go...the Science in the News Blog, a blog made by the Harvard graduate student group, Science in the News. While... keep content coming also keeps me on top of a variety of fields thereby broadening my knowledge. In addition...

...Interest (YGOI) in a controlled environment under various conditions. The first thing you'll need to decide...protein can be released from the resin and used for various binding or folding assays. Yeast vectors There ...large collection of yeast expression vectors with various markers and promoters. If you need to visualize... downstream effects.  And many more! We have a variety of other types of vectors for most applications...

...small region. This makes them useful for a wide variety of high-throughput experiments. There are currently... to observe clonal dynamics and cell lineage. Variations of this library allow tracking of transcription...’s also highlight a prokaryotic library: the Cultivarium MACKEREL Library, whose acronym means Modular...libraries have been used to systematically explore variations in the spike protein, teaching us more about ...

...impractical to catalog every variant of a feature. Instead, we identified common variants, and then crafted a ...threshold. This issue is addressed by adding more variants to the database. Another limitation is that by...augment the database with new fluorescent protein variants. The future of DNA feature identification What...

...should they decide to produce virus on their own.Harvard researcher Connie Cepko has been helping us with...viral preps are available now? There are a wide variety of tools already available from Addgene as ready-to-use...system. The first two can serve as controls in a variety of experiments, while rtTA can be used to activate...beginning our AAV viral services with these tools. A variety of chemogenetic tools from Bryan Roth’s lab are...

...immune reaction to the treatment. These responses vary depending on the person. However, in silico and ... antigen, pre-existing conditions, etc. that can vary widely between recipients.   In vitro prediction...of the human lymphatic system. PBMCs consist of various immune cells, and can be used as a whole or can...microscopic in-vitro models can be built that contain various components of the immune system. Figure...

...typically have a wide range of input quantities. This variation is problematic for conventional NGS library prep...range (1ng – 100ng) are normalized to two-fold variation above and below mean read count per sample, and...and the amount of information per read does not vary by DNA input amount. plexWell™ + Addgene = high quality...Boston University, and did postdoctoral work at Harvard Medical School and the University of Toronto. He...

...fused a different fluorescent protein to various Cas9 variants, SpCas9, NmCas9, and St1Cas9. These fusions...them. Thus, it has been widely adapted for a wide variety of applications: base editing, CRISPR activation... and results were consistent with copy number variation (CNV) analysis of the U2OS cells.   Figure...

... are mTagBFP2, EGFP or one of the improved GFP variants, mRuby2 or TagRFP-T, and an infrared fluorescent...In mammalian cells, one of the improved folding variants of EGFP like mEmerald or Clover is probably best...measurements. Here, EGFP outperforms the improved folding variants, presumably due to the lower growth temperature...fellow at the Bauer Center for Genomics Research at Harvard University.       ...

...expression (Zhou et al., 2017). The most common variant is an 11 bp deletion in the B or C domain of one... ITR sequences from different AAV serotypes that vary in their RBE and T-shaped hairpin loop sequences...non-AAV2 ITRs can be used to drive gene expression at varying capacities. AAV vectors containing the AAV2 ITRs...different combinations of ITR and capsid serotypes vary in their gene transfer efficiency. Our practical...

...the best advice is to try a couple of promising variants to check how they perform under your experimental... a fluorescent protein (fusion) there is huge variation in the fluorescence intensities of individual ... plasmid uptake by cells. To correct for this variation, a reference fluorescent protein that is expressed... from Addgene. Another way to dodge cellular variation observed in mammalian cells is to tag an endogenous...